Aligments for a candidate for metY in Dyella japonica UNC79MFTsu3.2

GapMind for Amino acid biosynthesis

Aligments for a candidate for metY in Dyella japonica UNC79MFTsu3.2

Align Cystathionine gamma-synthase/O-acetylhomoserine (thiol)-lyase; CGS/OAH thiolyase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase; EC 2.5.1.- (characterized)
to candidate N515DRAFT_4305 N515DRAFT_4305 cystathionine gamma-lyase

Query= SwissProt::O31631
         (373 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_4305 N515DRAFT_4305
           cystathionine gamma-lyase
          Length = 392

 Score =  310 bits (795), Expect = 3e-89
 Identities = 160/368 (43%), Positives = 226/368 (61%), Gaps = 18/368 (4%)

Query: 13  GNRSDEVTGTVSAPIYLSTAYRHRGIGESTGFDYVRTKNPTRQLVEDAIANLENGARGLA 72
           G   D  TG +  PIY ++ Y     G+  G++Y RT+NPTR   E  +A LE G  G A
Sbjct: 24  GQSPDPTTGAIMTPIYATSTYVQESPGKHKGYEYSRTQNPTRMAYEACVAALEGGVAGFA 83

Query: 73  FSSGMAAIQTIMALFKSGDELIVSSDLYGGTYRLFENEWKKY-GLTFHYDDFSDEDCLRS 131
           F SG+AA  T++ L  SG  +I   DLYGG+YRLFE   ++  GL F + D +D   L++
Sbjct: 84  FGSGLAAAATVLDLLDSGSHVIAMDDLYGGSYRLFERVRRRSAGLDFTFVDLNDAQALKA 143

Query: 132 KITPNTKAVFVETPTNPLMQEADIEHIARITKEHGLLLIVDNTFYTPVLQRPLELGADIV 191
            + PNTK ++ ETPTNP+++  D+  +A   K+HGL+L+VDNTF +P++QRP E GAD+V
Sbjct: 144 ALKPNTKMIWAETPTNPMLKLVDLAKVAAFAKKHGLILVVDNTFCSPMIQRPFESGADLV 203

Query: 192 IHSATKYLGGHNDLLAGLVVVKDERLGEEMFQHQNAIGAVLPPFDSWLLMRGMKTLSLRM 251
           +HSATKYL GH+D++ G+VV +++ L E M   QN++GAV  PFDS+L MRG+KTL LRM
Sbjct: 204 LHSATKYLNGHSDMVGGIVVAREQELAERMGFLQNSVGAVAGPFDSFLAMRGLKTLHLRM 263

Query: 252 RQHQANAQELAAFLEEQEEISDVLYPGK----------------GGMLSFRLQKE-EWVN 294
           + H  +A ELA +LE+  E+  V+YPG                 GG++S  ++       
Sbjct: 264 KAHCESALELAQWLEKHPEVERVIYPGLKSHPQHALARRQMHGFGGIISIEVKGGLRKAR 323

Query: 295 PFLKALKTICFAESLGGVESFITYPATQTHMDIPEEIRIANGVCNRLLRFSVGIEHAEDL 354
             L+       AESLGGVES I +PA  TH  +P   R   G+ + L+R SVG+E   DL
Sbjct: 324 RMLERCHLFALAESLGGVESLIEHPAIMTHASVPPANRKRLGISDSLIRLSVGVEDIADL 383

Query: 355 KEDLKQAL 362
           + +L +AL
Sbjct: 384 RSELAEAL 391


Lambda     K      H
   0.319    0.135    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 361
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 373
Length of database: 392
Length adjustment: 30
Effective length of query: 343
Effective length of database: 362
Effective search space:   124166
Effective search space used:   124166
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis