Align dihydroxy-acid dehydratase subunit (EC 4.2.1.9) (characterized)
to candidate N515DRAFT_2409 N515DRAFT_2409 dihydroxy-acid dehydratase
Query= metacyc::MONOMER-11919 (549 letters) >FitnessBrowser__Dyella79:N515DRAFT_2409 Length = 574 Score = 361 bits (926), Expect = e-104 Identities = 213/548 (38%), Positives = 318/548 (58%), Gaps = 12/548 (2%) Query: 7 KRGIQRAPHRSLLARCGLTDDDFE-KPFIGIANSYTDIVPGHIHLRELAEAVKEGVNAAG 65 + G Q +RS L GL +D F+ +P IGI N+++++ P + RELAE VK GV AG Sbjct: 17 REGKQGFYYRSWLKGLGLPNDMFDGRPVIGICNTWSELTPCNSSFRELAEHVKRGVYEAG 76 Query: 66 GVAFEFNTMAICDGIAMNHDGMKYSLASREIVADTVESMAMAHALDGLVLLPTCDKIVPG 125 G EF M++ + M M + R + + VE A+ +DG+VLL CDK P Sbjct: 77 GFPLEFPVMSLGE-TQMRPTAMLF----RNLASMDVEESIRANPIDGVVLLMGCDKTTPA 131 Query: 126 MLMAAARLDIPAIVVTGGPMLPGEFKGRKVDL-INVYEGVGTVSAGEMSEDELEELERCA 184 ++M AA +++P I ++GGP L G ++G+ + V V AGE++++E E E C Sbjct: 132 LMMGAASVNLPTIGLSGGPSLSGNWRGQPIGSGTGVIRMSEMVRAGELAQEEFVEAEACM 191 Query: 185 CPGPRSCAGLFTANTMACLTEALGMSLPGCATAHAVSSRKRQIARLSGKRIVEMVQENLK 244 SC + TA+TMA + EALG+SLP A AV SR+ ++A LSG+RIVEMV E+L+ Sbjct: 192 QRSKGSCMTMGTASTMASMVEALGLSLPENAAIPAVDSRRFRLAHLSGRRIVEMVHEDLR 251 Query: 245 PTMIMSQEAFENAVMVDLALGGSTNTTLHIPAIAAEIDGLNINLDLFDELSRVIPHIASI 304 + I+++ AFENA+ + A+GGSTN +H+ A+A + G+ + LD +D L +P + ++ Sbjct: 252 MSKILTRAAFENAIRANAAIGGSTNAVIHLLALAGRL-GVELALDDWDRLGSHLPCLVNL 310 Query: 305 SPAGEHMMLDLDRAGGIPAVLKTLEDHINRECVTCTGRTVQENIENVKVGHRDVIRPLDS 364 P+G+++M D AGG+PAVL+ + H++ +T GRT+ +NI +RDVIRP++ Sbjct: 311 KPSGDYLMEDFYYAGGLPAVLREIAPHLHLGALTANGRTLGDNIATAPCWNRDVIRPIEE 370 Query: 365 PVHSEGGLAILRGNLAPRGSVVKQGAVAEDMMVHEGPAKVFNSEDECMEAIFGGR--IDE 422 P+ E G+A+LRGNLAP G+V+K A + ++ H G A VF + D+ I IDE Sbjct: 371 PLKREAGIAVLRGNLAPDGAVIKPSAASAHLLQHRGRAVVFENIDDFKARIDDEALDIDE 430 Query: 423 GDVIVIRYEGPKGGPGMREMLNP--TSAIAGMGLERVALITDGRFSGGTRGPCVGHVSPE 480 V+V++ GP+G PGM E+ N + G+ + I+D R SG G V H SPE Sbjct: 431 TCVMVLKNCGPRGYPGMAEVGNMPLPPKLLRRGITDIVRISDARMSGTAYGTVVLHASPE 490 Query: 481 AMEDGPLAAVNDGDIIRIDIPSRKLEVDLSPREIEERLQSAVKPRRSVKGWLARYRKLAG 540 A G LA V DGD I +D+P R+L +DL+ E+ R + P +GW Y Sbjct: 491 AAAGGNLALVRDGDFIELDVPGRRLHLDLADDELARRRAAWKAPPPPERGWAKLYVDHVQ 550 Query: 541 SADTGAVL 548 A GA L Sbjct: 551 QAHLGADL 558 Lambda K H 0.319 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 845 Number of extensions: 37 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 549 Length of database: 574 Length adjustment: 36 Effective length of query: 513 Effective length of database: 538 Effective search space: 275994 Effective search space used: 275994 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory