Aligments for a candidate for argD in Herbaspirillum seropedicae SmR1

GapMind for Amino acid biosynthesis

Aligments for a candidate for argD in Herbaspirillum seropedicae SmR1

Align Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 (uncharacterized)
to candidate HSERO_RS23240 HSERO_RS23240 omega amino acid--pyruvate aminotransferase

Query= curated2:O27392
         (390 letters)



>FitnessBrowser__HerbieS:HSERO_RS23240
          Length = 442

 Score =  185 bits (469), Expect = 3e-51
 Identities = 140/425 (32%), Positives = 207/425 (48%), Gaps = 56/425 (13%)

Query: 16  QTYTRQPIVLSHGKGATVWDIEGNSYIDCFAGVAVNSIGHAHPKVALAICHQAQRLIHSS 75
           + +   P +L   +G    D  GN+ +D  AG+    +GHAHPK+  A+      L ++ 
Sbjct: 23  RNFKAAPRMLVSAEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVSAVQKSVATLDYAP 82

Query: 76  NIYYTREQV-ELAKLLTAISPH--DRVFFANSGAEANEGAIKLARKF------TGKSEII 126
           +         ELA+ L A + +    VF+  SG+EA + A+K+A  +        ++ +I
Sbjct: 83  SFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAYHRAKGNATRTRLI 142

Query: 127 AAENSFHGRTLATVTATG----QKKYSEPFRPLPEGFKHVPYG-------------DIGA 169
             E  +HG     ++  G    +K +S    P   G  H+P+              + GA
Sbjct: 143 GRERGYHGVGFGGMSVGGIGGNRKTFSTALLP---GVDHLPHTHNLEKNAFTKGEPEYGA 199

Query: 170 -MADAV--------GDETAAIILEPVQGEGGVIIPPEGYLKDVQELARQNDVLLILDEVQ 220
            +AD +            AA+I+EPV G  GV+IPP+GYLK ++E+  ++ +LLI DEV 
Sbjct: 200 HLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLKRLREICTKHGILLIFDEVI 259

Query: 221 TGFGRTGAMFASQLFGVEPDITTVAKAMGGG-YPIGAVLANERVAMAFEPGD------HG 273
           TGFGR G  FAS  F VEPDI T AK +  G  P+GAV     +  AF  G       HG
Sbjct: 260 TGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNGPDGIELFHG 319

Query: 274 STFGGNPWGCAAAIATIEVLMDEKLPERAAKMGSYFLGRLRQVLHGCDAVRDIRGVGLMI 333
            T+ G+P  CAA +A++EV   E + E A  +  YF       L G   V D+R +GL+ 
Sbjct: 320 YTYSGHPLACAAGLASLEVFQTEGVLENAQAVADYF-QEAAHALRGLPHVIDVRTIGLIA 378

Query: 334 GIEIDGECAGVVDA--------AREMGVLINCTAGKVIRIVPPLVIKKEEIDAAVDVLGH 385
           GIE+     G V A        A   G+LI  T G +I + PPL+I K++ID     L  
Sbjct: 379 GIEL-ASIPGRVGARAYDAFVRAFNDGILIRVT-GDIIALSPPLIINKQQIDELFGKLAA 436

Query: 386 VISDL 390
           ++  L
Sbjct: 437 ILKTL 441


Lambda     K      H
   0.320    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 459
Number of extensions: 21
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 390
Length of database: 442
Length adjustment: 31
Effective length of query: 359
Effective length of database: 411
Effective search space:   147549
Effective search space used:   147549
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis