Aligments for a candidate for dapF in Herbaspirillum seropedicae SmR1

GapMind for Amino acid biosynthesis

Aligments for a candidate for dapF in Herbaspirillum seropedicae SmR1

Align Diaminopimelate epimerase; DAP epimerase; PLP-independent amino acid racemase; EC 5.1.1.7 (characterized)
to candidate HSERO_RS21455 HSERO_RS21455 diaminopimelate epimerase

Query= SwissProt::P0A6K1
         (274 letters)



>FitnessBrowser__HerbieS:HSERO_RS21455
          Length = 287

 Score =  291 bits (745), Expect = 1e-83
 Identities = 152/281 (54%), Positives = 193/281 (68%), Gaps = 7/281 (2%)

Query: 1   MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY 60
           ++F+KMHG GNDF+V+DA+ Q++  SP   + +ADR  G+G DQ+LVVE      +DF Y
Sbjct: 3   IKFTKMHGAGNDFVVLDAINQSISLSPAQWQFIADRRFGIGADQMLVVEKARGEGVDFRY 62

Query: 61  RIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGE 120
           RI+NADG EV QCGNGAR F +FV  KGLT+KR IRV T +G +   + DD  + V+MG 
Sbjct: 63  RIYNADGGEVEQCGNGARAFVKFVTDKGLTSKRAIRVETMSGVIEPRLEDDGQITVDMGA 122

Query: 121 PNFEPSAVPFRA------NKAEKT-YIMRAAEQTILCGVVSMGNPHCVIQVDDVDTAAVE 173
           P   P+ VPF +      N+AE T + +  A +T    VVSMGNPH V  V+D + A V 
Sbjct: 123 PILTPADVPFDSHGLQHRNEAEDTLWPLDVAGKTTWISVVSMGNPHAVQVVEDSEAAPVL 182

Query: 174 TLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGL 233
           + GP++E HERFP+R N GFMQVV R H+ LRVYERGAGET ACG+GACAAV  GI++GL
Sbjct: 183 SEGPLIERHERFPKRVNAGFMQVVDRHHVNLRVYERGAGETLACGTGACAAVVAGIRRGL 242

Query: 234 LAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL 274
           L   V V+  GG L+IAW GPG  + MTGPAV VY+G I L
Sbjct: 243 LDSPVAVQTHGGVLNIAWAGPGEAVMMTGPAVSVYEGEIEL 283


Lambda     K      H
   0.323    0.140    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 255
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 274
Length of database: 287
Length adjustment: 26
Effective length of query: 248
Effective length of database: 261
Effective search space:    64728
Effective search space used:    64728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 47 (22.7 bits)

Align candidate HSERO_RS21455 HSERO_RS21455 (diaminopimelate epimerase)
to HMM TIGR00652 (dapF: diaminopimelate epimerase (EC 5.1.1.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00652.hmm
# target sequence database:        /tmp/gapView.22066.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00652  [M=270]
Accession:   TIGR00652
Description: DapF: diaminopimelate epimerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                  Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                  -----------
    2.5e-91  291.8   0.0    2.8e-91  291.6   0.0    1.0  1  lcl|FitnessBrowser__HerbieS:HSERO_RS21455  HSERO_RS21455 diaminopimelate ep


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__HerbieS:HSERO_RS21455  HSERO_RS21455 diaminopimelate epimerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  291.6   0.0   2.8e-91   2.8e-91       1     268 [.       3     281 ..       3     283 .. 0.92

  Alignments for each domain:
  == domain 1  score: 291.6 bits;  conditional E-value: 2.8e-91
                                  TIGR00652   1 meFlkmhGlgNdFvlvdevdeelvkeeaelvrkvcdrhtgvgaDgvllvep.sseeadvklrifNsDG 67 
                                                ++F+kmhG+gNdFv++d +++++  + +++ + ++dr++g+gaD++l+ve+   e  d+++ri+N+DG
  lcl|FitnessBrowser__HerbieS:HSERO_RS21455   3 IKFTKMHGAGNDFVVLDAINQSISLS-PAQWQFIADRRFGIGADQMLVVEKaRGEGVDFRYRIYNADG 69 
                                                68******************777766.***********************9666779*********** PP

                                  TIGR00652  68 SeaemCGNgiRcfakfvyekglkekkelsvetlaglikveveeenkkvkvdmgepkfkkeeiplt... 132
                                                 e+e+CGNg+R f+kfv++kgl++k+ ++vet +g+i+  +e+++ +++vdmg p ++++++p++   
  lcl|FitnessBrowser__HerbieS:HSERO_RS21455  70 GEVEQCGNGARAFVKFVTDKGLTSKRAIRVETMSGVIEPRLEDDG-QITVDMGAPILTPADVPFDshg 136
                                                *********************************************.*******************544 PP

                                  TIGR00652 133 .vekeeekeellalev.....l..vvdvGnPHlvvfvedvekldleelgklleaheefpegvNvefve 192
                                                 ++ +e +++l  l+v     +  vv++GnPH+v +ved e +++ + g+l+e he+fp++vN  f++
  lcl|FitnessBrowser__HerbieS:HSERO_RS21455 137 lQHRNEAEDTLWPLDVagkttWisVVSMGNPHAVQVVEDSEAAPVLSEGPLIERHERFPKRVNAGFMQ 204
                                                333333334444444477776268******************************************** PP

                                  TIGR00652 193 vkkedeiklrvyERGageTlaCGtGavAsavvalklgktkkkvtvhleggeLeievkedg.kvyltGp 259
                                                v++++++ lrvyERGageTlaCGtGa+A++v++++ g++++ v+v+++gg L+i +   g  v++tGp
  lcl|FitnessBrowser__HerbieS:HSERO_RS21455 205 VVDRHHVNLRVYERGAGETLACGTGACAAVVAGIRRGLLDSPVAVQTHGGVLNIAWAGPGeAVMMTGP 272
                                                ************************************************************99****** PP

                                  TIGR00652 260 avlvlegel 268
                                                av v+ege+
  lcl|FitnessBrowser__HerbieS:HSERO_RS21455 273 AVSVYEGEI 281
                                                *******97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (287 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 10.42
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis