Alignments for a candidate for proC in Herbaspirillum seropedicae SmR1

GapMind for Amino acid biosynthesis

Alignments for a candidate for proC in Herbaspirillum seropedicae SmR1

Align Pyrroline-5-carboxylate reductase; P5C reductase; P5CR; PCA reductase; EC 1.5.1.2 (characterized)
to candidate HSERO_RS18990 HSERO_RS18990 pyrroline-5-carboxylate reductase

Query= SwissProt::P22008
         (273 letters)



>FitnessBrowser__HerbieS:HSERO_RS18990
          Length = 260

 Score =  208 bits (529), Expect = 1e-58
 Identities = 123/260 (47%), Positives = 157/260 (60%), Gaps = 1/260 (0%)

Query: 14  MAASLIGGLRAQGVPAAQIRASDPGAEQRAKIAGEFAIDVVESNAEAVADADVVVLSVKP 73
           MAA+LIGGL  +   A  I   D  A+  A +   F + V  +   A+A ADVVVL+VKP
Sbjct: 1   MAAALIGGLVGKVAAAQDIHVVDLNAQALASLKDRFGVSVAPAMDGALASADVVVLAVKP 60

Query: 74  QAMKAVCQALAPALKPEQLIVSIAAGIPCASLEAWLGQPRPVVRCMPNTPALLRQGASGL 133
           Q M+ V  +L P +   QL+VSIAAGI    L  WL     +VR MPNTPAL+ +G +GL
Sbjct: 61  QQMRDVVGSLKPFIGA-QLVVSIAAGIRMVDLSRWLDGHEAIVRAMPNTPALIGKGITGL 119

Query: 134 YANAQVSAAQCEQAGQLLSAVGIALWLDDEAQIDAVTAVSGSGPAYFFLLMQAMTDAGEK 193
             + +VS  Q  QA  +L AVG  LWL DEAQIDAVTAVSGSGPAY F  ++AM  A  +
Sbjct: 120 VPHEKVSTLQRAQADSILKAVGQTLWLSDEAQIDAVTAVSGSGPAYVFYFLEAMEQAAAQ 179

Query: 194 LGLSRETASRLTLQTALGAAQMALSSEVEPAELRRRVTSPNGTTEAAIKSFQANGFEALV 253
           LGLS      L   T  GA ++A  SE   A LR RVTS  GTT AA+ S + +G +  +
Sbjct: 180 LGLSPAEGLELAKATFEGATELARRSEESVATLRERVTSKGGTTYAALTSLEQSGVKDAI 239

Query: 254 EQALNAASQRSAELAEQLGQ 273
            +AL AA+ RS EL ++ G+
Sbjct: 240 VKALQAAAVRSKELGDEFGK 259


Lambda     K      H
   0.315    0.127    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 183
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 273
Length of database: 260
Length adjustment: 25
Effective length of query: 248
Effective length of database: 235
Effective search space:    58280
Effective search space used:    58280
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)

Align candidate HSERO_RS18990 HSERO_RS18990 (pyrroline-5-carboxylate reductase)
to HMM TIGR00112 (proC: pyrroline-5-carboxylate reductase (EC 1.5.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00112.hmm
# target sequence database:        /tmp/gapView.23309.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00112  [M=263]
Accession:   TIGR00112
Description: proC: pyrroline-5-carboxylate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                  Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                  -----------
    1.7e-78  249.9   7.5    1.9e-78  249.7   7.5    1.0  1  lcl|FitnessBrowser__HerbieS:HSERO_RS18990  HSERO_RS18990 pyrroline-5-carbox


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__HerbieS:HSERO_RS18990  HSERO_RS18990 pyrroline-5-carboxylate reductase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  249.7   7.5   1.9e-78   1.9e-78       9     263 .]       1     253 [.       1     253 [. 0.98

  Alignments for each domain:
  == domain 1  score: 249.7 bits;  conditional E-value: 1.9e-78
                                  TIGR00112   9 mgeallsgllkkgakakkeilvierseeklaalakelgvevtsdaeeavkeadvvllavKPqdleevl 76 
                                                m++al+ gl+ k ++a+++i+v++ ++++la l +++gv+v+ + + a + advv+lavKPq++++v+
  lcl|FitnessBrowser__HerbieS:HSERO_RS18990   1 MAAALIGGLVGK-VAAAQDIHVVDLNAQALASLKDRFGVSVAPAMDGALASADVVVLAVKPQQMRDVV 67 
                                                9**********8.5569*************************************************** PP

                                  TIGR00112  77 aelkseektkeklliSilAGvtiekleqlleaekrvvRvmPNtaakvgagvtaiaassevseeqkelv 144
                                                 +lk    + ++l++Si+AG+++ +l+++l++++++vR+mPNt+a +g+g+t+++ +++vs  q++++
  lcl|FitnessBrowser__HerbieS:HSERO_RS18990  68 GSLKP--FIGAQLVVSIAAGIRMVDLSRWLDGHEAIVRAMPNTPALIGKGITGLVPHEKVSTLQRAQA 133
                                                ****9..6689*************************************************999***** PP

                                  TIGR00112 145 eellkavGkvveve.eklldavtalsGSgPAfvflliealadagvklGLpreeakelaaqtlkGaakl 211
                                                +++lkavG++++++ e+++davta+sGSgPA+vf+++ea+++a+ +lGL+ +e +ela++t++Ga +l
  lcl|FitnessBrowser__HerbieS:HSERO_RS18990 134 DSILKAVGQTLWLSdEAQIDAVTAVSGSGPAYVFYFLEAMEQAAAQLGLSPAEGLELAKATFEGATEL 201
                                                **************9***************************************************** PP

                                  TIGR00112 212 leesgehpalLkdkVtsPgGtTiaglavLeekgvrsavieaveaavkrseeL 263
                                                  +s+e+ a+L+++Vts+gGtT a+l +Le++gv++a+ +a++aa+ rs+eL
  lcl|FitnessBrowser__HerbieS:HSERO_RS18990 202 ARRSEESVATLRERVTSKGGTTYAALTSLEQSGVKDAIVKALQAAAVRSKEL 253
                                                **************************************************98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (263 nodes)
Target sequences:                          1  (260 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 9.64
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis