Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized)
to candidate HSERO_RS16705 HSERO_RS16705 dihydroxy-acid dehydratase
Query= curated2:A8AB39 (552 letters) >FitnessBrowser__HerbieS:HSERO_RS16705 Length = 578 Score = 371 bits (953), Expect = e-107 Identities = 219/530 (41%), Positives = 328/530 (61%), Gaps = 16/530 (3%) Query: 31 RPLIGVANSWNEIVPGHVHLDKVAEAVKAGIRMAGGTPLEFGTIAVCDGIAMGHEGMRYS 90 RP+IG+ N+++E+ P + H +AE VK GI AGG PLEF +++ + + + M Y Sbjct: 43 RPVIGICNTYSELTPCNSHFRALAEQVKIGIWEAGGFPLEFPVMSLGETL-LRPTAMLY- 100 Query: 91 LPSREVIADTVEIMVEAHRLDAVVMVTNCDKITPGFLLAAARLEVPVILINGGPMMPGVY 150 R + + VE + A+ LD VV++ CDK TP L+ AA ++VP I ++GGPM+ G Y Sbjct: 101 ---RNLASMDVEESIRANPLDGVVLLMGCDKTTPALLMGAASVDVPTIGVSGGPMLSGKY 157 Query: 151 GKERIDFKDLMERMNVLIKEGRT--EELRKLEESALPGPGSCAGLFTANTMNMLSEAMGL 208 + + +M+ ++ G+ EE + E G C + TA+TM + EA+G+ Sbjct: 158 RGRELGSGTGVWQMSEDVRAGKMTQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGV 217 Query: 209 MLPGASTVPAVEARRLWYAKLTGMRIVKMVEEGLTPDKILTRKALENAIAVDMALGGSTN 268 LP + +PAV+ARR A+ G RIV+MVEE L KILTR+A ENAI V+ A+GGSTN Sbjct: 218 GLPHNAAIPAVDARRNVLARNAGRRIVQMVEEDLVLSKILTRQAFENAIRVNAAIGGSTN 277 Query: 269 SVLHLEALAYELGIDLPLEVFDEISRKVPHIASISPSGRHFVVDLDRAGGIPAVLKELGE 328 +V+HL A+A +GI L L+ +D+I +++P + + PSG+ + D AGG+PAV+++L Sbjct: 278 AVIHLLAIAGRIGIKLDLKDWDDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQL-- 335 Query: 329 AGLIHKDALTVTGKTVWENVKDAAVLDREVIRPLDNPYSPFGGLAILKGSLAPNGAVVKA 388 +I K ALT GKT+WEN +DA + EVIR D P+ G+AILKG+LAP+GAV+K Sbjct: 336 ESVIDKTALTANGKTLWENCQDAPNWNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKP 395 Query: 389 SAVKRELWKFKGVARVFDREEDAVKAI--RGGEIEPGTVIVIRYEGPRGGPGMREM--LT 444 SA L K +G A VF+ +D K I +++ V+V++ GP+G PGM E + Sbjct: 396 SAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMP 455 Query: 445 ATAAVMALGLGDKVALVTDGRFSGATRGPAIGHVSPEAAAGGPIALVQDGDEIVIDIEKR 504 ++ G+ D V V+D R SG G + HVSPEAAAGGP+A+VQ+GD I +D+E R Sbjct: 456 LPPKILRKGITDMVR-VSDARMSGTAYGTVVLHVSPEAAAGGPLAVVQNGDFIELDVEAR 514 Query: 505 RLDLLVDEKELEERRARW-KPKVKP-LRRGILRRYAKMALSADKGGALEY 552 +L L V ++EL RRA+W KP++ P ++RG ++ Y A++G L++ Sbjct: 515 KLHLDVSDEELARRRAQWQKPELPPQMQRGWVKLYVDHVQQANQGADLDF 564 Lambda K H 0.319 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 812 Number of extensions: 36 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 552 Length of database: 578 Length adjustment: 36 Effective length of query: 516 Effective length of database: 542 Effective search space: 279672 Effective search space used: 279672 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory