Aligments for a candidate for aspS2 in Escherichia coli BW25113

GapMind for Amino acid biosynthesis

Aligments for a candidate for aspS2 in Escherichia coli BW25113

Align Aspartate--tRNA(Asp/Asn) ligase; EC 6.1.1.23; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS (uncharacterized)
to candidate 15054 b0930 asparaginyl-tRNA synthetase (NCBI)

Query= curated2:Q8TXG4
         (431 letters)



>lcl|FitnessBrowser__Keio:15054 b0930 asparaginyl-tRNA synthetase
           (NCBI)
          Length = 466

 Score =  179 bits (455), Expect = 1e-49
 Identities = 137/453 (30%), Positives = 217/453 (47%), Gaps = 58/453 (12%)

Query: 18  EVRLAGWVHEVRDL-GGIKFVLLRDRT------GIVQLTLPKQKVPKETFEKVPKLTKES 70
           EV + GWV   RD   GI F+ + D +       ++  +LP         E V +LT   
Sbjct: 19  EVTVRGWVRTRRDSKAGISFLAVYDGSCFDPVQAVINNSLPNYN------EDVLRLTTGC 72

Query: 71  VIRVEGTVQANEKAPGGVEVIPQRIEV---LSESDTHLPLDPTGKVDADLDTRLDARVLD 127
            + V G V A+       E+   ++EV   + + DT+    P       ++   +   L 
Sbjct: 73  SVIVTGKVVASPGQGQQFEIQASKVEVAGWVEDPDTY----PMAAKRHSIEYLREVAHLR 128

Query: 128 LRREEPQAIFKIRNVVTTAIREFLEERGFIEVHTPKIIASATEGGTELFPVV-------- 179
            R     A+ ++R+ +  A+  F  E+GF  V TP I AS TEG  E+F V         
Sbjct: 129 PRTNLIGAVARVRHTLAQALHRFFNEQGFFWVSTPLITASDTEGAGEMFRVSTLDLENLP 188

Query: 180 ------------YFERDAYLAQSPQLYKQMLMAAGFERVYEIGPIFRAEEHNTRRHLNEA 227
                       +F ++++L  S QL  +   A    ++Y  GP FRAE  NT RHL E 
Sbjct: 189 RNDQGKVDFDKDFFGKESFLTVSGQLNGETY-ACALSKIYTFGPTFRAENSNTSRHLAEF 247

Query: 228 ISVDIEMSFIESEEDVMRVLEELLAHVFRKVREECEKEL---------EALDRELPELET 278
             ++ E++F     D+  + E +L +VF+ V EE   ++         +A+ R    +E 
Sbjct: 248 WMLEPEVAFANLN-DIAGLAEAMLKYVFKAVLEERADDMKFFAERVDKDAVSRLERFIEA 306

Query: 279 PFERITYEETLDLLSEHGIEVE----WGEDLPTEAERKLGEI-FEEPFFITEWPRETRPF 333
            F ++ Y + + +L   G + E    WG DL +E ER L E  F+ P  +  +P++ + F
Sbjct: 307 DFAQVDYTDAVTILENCGRKFENPVYWGVDLSSEHERYLAEEHFKAPVVVKNYPKDIKAF 366

Query: 334 YTMAKDDEVTTA-FDLMYQGL-ELASGAQREHRYDVLVRQIEEQGLSPEDFRHYLEAFKY 391
           Y    +D  T A  D++  G+ E+  G+QRE R DVL  ++ E GL+ ED+  Y +  +Y
Sbjct: 367 YMRLNEDGKTVAAMDVLAPGIGEIIGGSQREERLDVLDERMLEMGLNKEDYWWYRDLRRY 426

Query: 392 GMPPHGGWGLGLERTLMTITGAENIREVTLFPR 424
           G  PH G+GLG ER +  +TG +N+R+V  FPR
Sbjct: 427 GTVPHSGFGLGFERLIAYVTGVQNVRDVIPFPR 459


Lambda     K      H
   0.318    0.138    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 486
Number of extensions: 32
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 431
Length of database: 466
Length adjustment: 33
Effective length of query: 398
Effective length of database: 433
Effective search space:   172334
Effective search space used:   172334
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis