Align 3-dehydroquinate dehydratase (EC 4.2.1.10) (characterized)
to candidate 15812 b1693 3-dehydroquinate dehydratase (NCBI)
Query= BRENDA::P05194 (252 letters) >FitnessBrowser__Keio:15812 Length = 252 Score = 481 bits (1239), Expect = e-141 Identities = 252/252 (100%), Positives = 252/252 (100%) Query: 1 MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDILEWRVDHYADLSNVES 60 MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDILEWRVDHYADLSNVES Sbjct: 1 MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDILEWRVDHYADLSNVES 60 Query: 61 VMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNRAAIDSGLVDMIDLELFTG 120 VMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNRAAIDSGLVDMIDLELFTG Sbjct: 61 VMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNRAAIDSGLVDMIDLELFTG 120 Query: 121 DDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRKMQSFDADIPKIALMPQSTSD 180 DDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRKMQSFDADIPKIALMPQSTSD Sbjct: 121 DDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRKMQSFDADIPKIALMPQSTSD 180 Query: 181 VLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEVFGSAATFGAVKKASAPGQISVN 240 VLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEVFGSAATFGAVKKASAPGQISVN Sbjct: 181 VLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEVFGSAATFGAVKKASAPGQISVN 240 Query: 241 DLRTVLTILHQA 252 DLRTVLTILHQA Sbjct: 241 DLRTVLTILHQA 252 Lambda K H 0.317 0.130 0.351 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 360 Number of extensions: 3 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 252 Length of database: 252 Length adjustment: 24 Effective length of query: 228 Effective length of database: 228 Effective search space: 51984 Effective search space used: 51984 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
Align candidate 15812 b1693 (3-dehydroquinate dehydratase (NCBI))
to HMM TIGR01093 (aroD: 3-dehydroquinate dehydratase, type I (EC 4.2.1.10))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01093.hmm # target sequence database: /tmp/gapView.11220.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01093 [M=229] Accession: TIGR01093 Description: aroD: 3-dehydroquinate dehydratase, type I Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2e-91 292.2 0.8 2.3e-91 292.0 0.8 1.0 1 lcl|FitnessBrowser__Keio:15812 b1693 3-dehydroquinate dehydrata Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Keio:15812 b1693 3-dehydroquinate dehydratase (NCBI) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 292.0 0.8 2.3e-91 2.3e-91 1 228 [. 17 245 .. 17 246 .. 0.99 Alignments for each domain: == domain 1 score: 292.0 bits; conditional E-value: 2.3e-91 TIGR01093 1 kilvpltakdleealeelekikevgaDivElRvDllkdvsseksveavaeqlsellkdlplilTiRtqkeGGkfkgdee 79 ki+v+l+akd++ +++e+ +++e ++Di+E+RvD+++d+s+ +sv+a+a+ l+e+++++pl++T+R++keGG+++ ++e lcl|FitnessBrowser__Keio:15812 17 KIIVSLMAKDIASVKSEALAYREADFDILEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTE 95 69***************************************************************************** PP TIGR01093 80 erleelkeaieknlvdlvDiElkleeeavkelikeakkaktkiilSnHdfektpskeelverlekaqsldaDivKiavm 158 ++++++++ai+++lvd++D+El++ +++vke++++a+++++k+++SnHdf+ktp++ee+++rl+k+qs+daDi+Kia m lcl|FitnessBrowser__Keio:15812 96 AYIALNRAAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRKMQSFDADIPKIALM 174 ******************************************************************************* PP TIGR01093 159 aksieDvltLleitlkveeek.dkplialsMgekGkisRvlgavlgsvltfgslgkasAPGQisvkelrel 228 ++s++DvltLl++tl+++e++ d+p+i++sM+++G isR++g+v+gs++tfg+++kasAPGQisv++lr + lcl|FitnessBrowser__Keio:15812 175 PQSTSDVLTLLAATLEMQEQYaDRPIITMSMAKTGVISRLAGEVFGSAATFGAVKKASAPGQISVNDLRTV 245 ********************************************************************986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (229 nodes) Target sequences: 1 (252 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 6.94 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory