Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate Ga0059261_0082 Ga0059261_0082 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A (EC 6.3.5.-)
Query= SwissProt::O06491 (485 letters) >FitnessBrowser__Korea:Ga0059261_0082 Length = 494 Score = 438 bits (1127), Expect = e-127 Identities = 233/485 (48%), Positives = 324/485 (66%), Gaps = 10/485 (2%) Query: 8 ITELKQLIHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDEAVDGRSE 67 + ++ + E ++ D ++ A ++ AF+ + A A A+ D A Sbjct: 9 VAAIRDGVRSGEFTAIEVADAFITKVSAAK-QLNAFIVETPDHAIAAARTADAARAAGET 67 Query: 68 HGLLFGMPIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGKLNMDEF 127 L G+PIG+KD TKG++TT +S +LE F P+Y++TV Q+L DA A +GKLN+D+F Sbjct: 68 LKPLAGVPIGMKDLFCTKGVQTTAASHMLEGFTPVYESTVSQKLWDAGAGMLGKLNLDQF 127 Query: 128 AMGSSTENSAYKLTKNPW------NLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQ 181 AMGSS E S + +PW N PGGSSGGS++A+AA VP + G+DTGGSIRQ Sbjct: 128 AMGSSNETSYFGNVISPWRRKDGGNAALAPGGSSGGSSSAIAARLVPAATGTDTGGSIRQ 187 Query: 182 PASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSA 241 PA+F G+ G+KPTYGR SR+G+VAFASSLDQ GP+ R V D A +L+A++G D D+TS Sbjct: 188 PAAFTGISGIKPTYGRCSRWGVVAFASSLDQAGPMARDVRDCAIMLEAMAGFDPKDATSL 247 Query: 242 NVDVPDFLSSLTGDIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPH 301 ++ VP++ + L+ D++G K+ +PKEY +G+ E + L+ GA EVSLPH Sbjct: 248 DMAVPNWEAGLSADLRGKKVGIPKEYRVDGMPAEIEALWQQGIDWLKDAGAEIVEVSLPH 307 Query: 302 SKYALATYYLLSSSEASANLARFDGIRYGYR-TDNADNLIDLYKQTRAEGFGNEVKRRIM 360 +KYAL YY+++ +EAS+NLAR+DG+RYG R + NL ++Y TRA GFG EV+RRI+ Sbjct: 308 TKYALPAYYIIAPAEASSNLARYDGVRYGQRDLPDGANLQEMYAATRAAGFGPEVQRRIL 367 Query: 361 LGTFALSSGYYDAYYKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLT 420 +GT+ LS+G+YDAYY +AQKVRTLI +DFE + + D+++ PT P+ AF +GE + DPL Sbjct: 368 IGTYVLSAGFYDAYYTQAQKVRTLIAQDFEKAWAQCDLLLTPTAPSAAFALGEKSADPLA 427 Query: 421 MYANDILTIPVNLAGVPGISVPCGL-ADGLPLGLQIIGKHFDESTVYRVAHAFEQATDHH 479 MY ND+ T+P +LAG+P +SVP GL DGLPLGLQIIGK FDE V + A EQ Sbjct: 428 MYLNDVFTVPSSLAGIPAMSVPGGLDKDGLPLGLQIIGKPFDEQGVLNASLAIEQRAG-F 486 Query: 480 KAKPE 484 AKPE Sbjct: 487 TAKPE 491 Lambda K H 0.315 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 573 Number of extensions: 18 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 485 Length of database: 494 Length adjustment: 34 Effective length of query: 451 Effective length of database: 460 Effective search space: 207460 Effective search space used: 207460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
Align candidate Ga0059261_0082 Ga0059261_0082 (aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A (EC 6.3.5.-))
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00132.hmm # target sequence database: /tmp/gapView.19413.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00132 [M=466] Accession: TIGR00132 Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.3e-170 554.1 0.0 1.4e-170 553.9 0.0 1.0 1 lcl|FitnessBrowser__Korea:Ga0059261_0082 Ga0059261_0082 aspartyl/glutamyl Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Korea:Ga0059261_0082 Ga0059261_0082 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A ( # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 553.9 0.0 1.4e-170 1.4e-170 5 463 .. 16 483 .. 12 486 .. 0.96 Alignments for each domain: == domain 1 score: 553.9 bits; conditional E-value: 1.4e-170 TIGR00132 5 lkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva..ke.kklagipiavKdnia 70 + + e+++ ev+++++ +++a k+ +naf+ t+++a+++a+ d++ a ++ k+lag+pi++Kd ++ lcl|FitnessBrowser__Korea:Ga0059261_0082 16 VRSGEFTAIEVADAFITKVSAAKQ-LNAFIVETPDHAIAAARTADAARAagETlKPLAGVPIGMKDLFC 83 566778889999********9999.********************9888865567************** PP TIGR00132 71 vkdiettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknP......en 133 +k+++tt+aS++Le+++++y++tV ++l +aga ++Gk NlD FamGss etS+fg + P n lcl|FitnessBrowser__Korea:Ga0059261_0082 84 TKGVQTTAASHMLEGFTPVYESTVSQKLWDAGAGMLGKLNLDQFAMGSSNETSYFGNVISPwrrkdgGN 152 *************************************************************43333335 PP TIGR00132 134 eervpGGSsgGsaaavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGi 202 + pGGSsgGs+ a+aa lvp a g+DTGgSiRqPA+f+g+ G+KPtYG+ SR+G+va+asSldq G+ lcl|FitnessBrowser__Korea:Ga0059261_0082 153 AALAPGGSSGGSSSAIAARLVPAATGTDTGGSIRQPAAFTGISGIKPTYGRCSRWGVVAFASSLDQAGP 221 6789***************************************************************** PP TIGR00132 203 lakkvedialvldvisgkDkkDstslevkveelleelkkdlkglkvgvvkelseesldkevkekfekll 271 +a++v d a++l++++g D kD tsl+++v+++ l +dl+g kvg+ ke++ ++++ e+++ +++ + lcl|FitnessBrowser__Korea:Ga0059261_0082 222 MARDVRDCAIMLEAMAGFDPKDATSLDMAVPNWEAGLSADLRGKKVGIPKEYRVDGMPAEIEALWQQGI 290 ********************************************************************* PP TIGR00132 272 ekleelgaeivevslpsvklalaiYyiispsEassnlarydgiryGkrve.elkslkelyaktRsegfg 339 + l++ gaeivevslp+ k+al++Yyii+p+Eassnlarydg+ryG+r + +l+e+ya+tR++gfg lcl|FitnessBrowser__Korea:Ga0059261_0082 291 DWLKDAGAEIVEVSLPHTKYALPAYYIIAPAEASSNLARYDGVRYGQRDLpDGANLQEMYAATRAAGFG 359 ************************************************7526667************** PP TIGR00132 340 eevkrRimlGayalskeyydkyykkAqkvrtliidefeklfeevDvivsptaptlafklgekaedplem 408 ev+rRi++G+y+ls++ yd+yy++Aqkvrtli+++fek++ ++D++++ptap af lgek +dpl+m lcl|FitnessBrowser__Korea:Ga0059261_0082 360 PEVQRRILIGTYVLSAGFYDAYYTQAQKVRTLIAQDFEKAWAQCDLLLTPTAPSAAFALGEKSADPLAM 428 ********************************************************************* PP TIGR00132 409 ylsDvltvpanlaGlpaisvPlgkkekglpiGlqiigkafddkkllsvakaleqa 463 yl+Dv+tvp +laG+pa+svP g +++glp+Glqiigk fd++ +l+++ a+eq+ lcl|FitnessBrowser__Korea:Ga0059261_0082 429 YLNDVFTVPSSLAGIPAMSVPGGLDKDGLPLGLQIIGKPFDEQGVLNASLAIEQR 483 ****************************************************997 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (466 nodes) Target sequences: 1 (494 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 11.96 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory