Alignments for a candidate for dapF in Sphingomonas koreensis DSMZ 15582

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapF in Sphingomonas koreensis DSMZ 15582

Align Diaminopimelate epimerase; DAP epimerase; EC 5.1.1.7; PLP-independent amino acid racemase (uncharacterized)
to candidate Ga0059261_2394 Ga0059261_2394 diaminopimelate epimerase (EC 5.1.1.7)

Query= curated2:Q97FV2
         (333 letters)



>FitnessBrowser__Korea:Ga0059261_2394
          Length = 315

 Score =  171 bits (433), Expect = 2e-47
 Identities = 105/290 (36%), Positives = 158/290 (54%), Gaps = 6/290 (2%)

Query: 12  VEINILKCHGTGNDFILIDEYNNNYNLDDETRRDIAIQACNRAKFIGGDGILFV-QKSDI 70
           + +   KCHG+GNDF+L+D   +   L D     IA   C R   +G DG+L + + SD 
Sbjct: 4   MRLTFTKCHGSGNDFVLLDARGST--LSDSEWGRIARSLCERDGPVGADGLLLLGEGSDG 61

Query: 71  CDAKMRIFNADGSEAEMCGNGLRCVGRYVIEMLNKESVEIETLKSKYWVKLQEDIYEGVK 130
                ++ NADGS  E C NGLRC  R   E+L  E+  ++   S   V    D+  GV 
Sbjct: 62  AQFAQKVVNADGSIPETCLNGLRCTARAGFELLGIEAATVQLKTSDAQVARDADLAPGVY 121

Query: 131 TVKIDIKSVSLDVKTLPLNYKKEKLIFDKIPELSDEFDFTAVSITNPHLIAIVNNIDSDK 190
           TV+  +   S D +   +N     +I   +P L +  +FTAV++ NPHLIA V+ +D  +
Sbjct: 122 TVRTTVGPASTDPQAAGVNLAAP-VIDAPVPGLPNGRNFTAVAMPNPHLIAFVDTVDVAE 180

Query: 191 LVEIGKKGNSTKSVLPQGVNVSFVKVIDSSNIYVKTYERGVGLTKSCGTAMTASSIVSCI 250
           L  +G    +   +L    NVSFV  +    ++V+TYERGVGLT +CGTAM A++ V+ +
Sbjct: 181 LTALGDWCEAGPDLLADRANVSFV-ALRPDGLFVQTYERGVGLTNACGTAMGAATHVAGL 239

Query: 251 GEKVQFDNAINVYNDGGAIKTIVHKDSNGNYSVDFIGNATFIFEGTMELD 300
             ++ F   I V+N GG +K      + G+ +V   GNATF ++G +E+D
Sbjct: 240 TGRIPFGAWITVHNPGGRVKARAEGPAGGD-AVTIAGNATFEWDGEIEID 288


Lambda     K      H
   0.317    0.136    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 231
Number of extensions: 9
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 333
Length of database: 315
Length adjustment: 28
Effective length of query: 305
Effective length of database: 287
Effective search space:    87535
Effective search space used:    87535
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 48 (23.1 bits)

Align candidate Ga0059261_2394 Ga0059261_2394 (diaminopimelate epimerase (EC 5.1.1.7))
to HMM TIGR00652 (dapF: diaminopimelate epimerase (EC 5.1.1.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00652.hmm
# target sequence database:        /tmp/gapView.10947.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00652  [M=270]
Accession:   TIGR00652
Description: DapF: diaminopimelate epimerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
      4e-48  150.1   0.0    4.8e-48  149.9   0.0    1.1  1  lcl|FitnessBrowser__Korea:Ga0059261_2394  Ga0059261_2394 diaminopimelate e


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Korea:Ga0059261_2394  Ga0059261_2394 diaminopimelate epimerase (EC 5.1.1.7)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  149.9   0.0   4.8e-48   4.8e-48       2     268 ..       7     285 ..       6     287 .. 0.89

  Alignments for each domain:
  == domain 1  score: 149.9 bits;  conditional E-value: 4.8e-48
                                 TIGR00652   2 eFlkmhGlgNdFvlvdevdeelvkee.aelvrkvcdrhtgvgaDgvllvepsseeadvklrifNsDGSe 69 
                                               +F+k hG+gNdFvl+d + ++l  +e  +++r++c+r   vgaDg+ll+ ++s+ a++  ++ N+DGS 
  lcl|FitnessBrowser__Korea:Ga0059261_2394   7 TFTKCHGSGNDFVLLDARGSTLSDSEwGRIARSLCERDGPVGADGLLLLGEGSDGAQFAQKVVNADGSI 75 
                                               7******************999999999***************************************** PP

                                 TIGR00652  70 aemCGNgiRcfakfvyekglkekkelsvetlaglikveveeen..kkvkvdmgepkfkkeeipltveke 136
                                               +e C Ng+Rc a+   e  + e  +++++t   ++      +    +v+  +g ++  +++  ++ +  
  lcl|FitnessBrowser__Korea:Ga0059261_2394  76 PETCLNGLRCTARAGFELLGIEAATVQLKTSDAQVARDADLAPgvYTVRTTVGPASTDPQAAGVNLAAP 144
                                               *************9999999988999999998888777766556899********99999888886555 PP

                                 TIGR00652 137 eekeellalev...l.vvdvGnPHlvvfvedvekldleelgklleaheef.pegvNvefvevkkedeik 200
                                                 +  +  l     + +v + nPHl+ fv+ v+ ++l+ lg   ea ++  +++ Nv fv     d ++
  lcl|FitnessBrowser__Korea:Ga0059261_2394 145 VIDAPVPGLPNgrnFtAVAMPNPHLIAFVDTVDVAELTALGDWCEAGPDLlADRANVSFVALRP-DGLF 212
                                               5544443333344546*********************************99*********8776.99** PP

                                 TIGR00652 201 lrvyERGageTlaCGtGavAsavvalklgktkkk..vtvhleggeLeievkedg...kvyltGpavlvl 264
                                               +++yERG+g T aCGt + A+  va  +g+ +    +tvh +gg+++ + +       v + G+a++  
  lcl|FitnessBrowser__Korea:Ga0059261_2394 213 VQTYERGVGLTNACGTAMGAATHVAGLTGRIPFGawITVHNPGGRVKARAEGPAggdAVTIAGNATFEW 281
                                               ******************************999888************999865566788999998888 PP

                                 TIGR00652 265 egel 268
                                               +ge+
  lcl|FitnessBrowser__Korea:Ga0059261_2394 282 DGEI 285
                                               8876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (315 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 11.90
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis