GapMind for Amino acid biosynthesis


L-histidine biosynthesis in Klebsiella michiganensis M5al

Best path

prs, hisG, hisI, hisE, hisA, hisF, hisH, hisB, hisC, hisN, hisD

Also see fitness data for the top candidates


Overview: Histidine biosynthesis in GapMind is based on the MetaCyc pathway (link).

11 steps (11 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
prs ribose-phosphate diphosphokinase BWI76_RS17460 BWI76_RS25955
hisG ATP phosphoribosyltransferase BWI76_RS18920
hisI phosphoribosyl-ATP pyrophosphatase BWI76_RS18955 BWI76_RS18925
hisE phosphoribosyl-AMP cyclohydrolase BWI76_RS18955 BWI76_RS18925
hisA isomerase HisA BWI76_RS18945 BWI76_RS18950
hisF IGP synthase, cyclase subunit BWI76_RS18950 BWI76_RS18945
hisH IGP synthase, amidotransferase subunit BWI76_RS18940 BWI76_RS23010
hisB IGP dehydratase BWI76_RS18935
hisC histidinol-phosphate aminotransferase BWI76_RS18930
hisN histidinol-phosphate phosphatase BWI76_RS06160 BWI76_RS18935
hisD histidinol dehydrogenase BWI76_RS18925

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.



Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory