GapMind for Amino acid biosynthesis

 

Aligments for a candidate for dapF in Klebsiella michiganensis M5al

Align Diaminopimelate epimerase; DAP epimerase; PLP-independent amino acid racemase; EC 5.1.1.7 (characterized)
to candidate BWI76_RS01200 BWI76_RS01200 diaminopimelate epimerase

Query= SwissProt::P0A6K1
         (274 letters)



>lcl|FitnessBrowser__Koxy:BWI76_RS01200 BWI76_RS01200
           diaminopimelate epimerase
          Length = 274

 Score =  535 bits (1378), Expect = e-157
 Identities = 258/274 (94%), Positives = 266/274 (97%)

Query: 1   MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY 60
           MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY
Sbjct: 1   MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQLLVVEPPYDPELDFHY 60

Query: 61  RIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLTVTDDDLVRVNMGE 120
           RIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVST+NGRMVL+VT+DDLVRVNMGE
Sbjct: 61  RIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTSNGRMVLSVTEDDLVRVNMGE 120

Query: 121 PNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHCVIQVDDVDTAAVETLGPVLE 180
           PNFEPS VPFRANKAEKTYIMRAAEQT+LCGVVSMGNPHCVIQVDDV+TA VETLGPV+E
Sbjct: 121 PNFEPSQVPFRANKAEKTYIMRAAEQTVLCGVVSMGNPHCVIQVDDVETATVETLGPVME 180

Query: 181 SHERFPERANIGFMQVVKREHIRLRVYERGAGETQACGSGACAAVAVGIQQGLLAEEVRV 240
           +HERFPERANIGFMQV+ R HIRLRVYERGAGETQACGSGACAA AVGIQQGLL EEVRV
Sbjct: 181 NHERFPERANIGFMQVISRSHIRLRVYERGAGETQACGSGACAAAAVGIQQGLLGEEVRV 240

Query: 241 ELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL 274
           ELPGGRLDIAWKGPG PLYMTGPA HVYDGFIHL
Sbjct: 241 ELPGGRLDIAWKGPGQPLYMTGPAAHVYDGFIHL 274


Lambda     K      H
   0.323    0.140    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 376
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 274
Length of database: 274
Length adjustment: 25
Effective length of query: 249
Effective length of database: 249
Effective search space:    62001
Effective search space used:    62001
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 47 (22.7 bits)

Align candidate BWI76_RS01200 BWI76_RS01200 (diaminopimelate epimerase)
to HMM TIGR00652 (dapF: diaminopimelate epimerase (EC 5.1.1.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00652.hmm
# target sequence database:        /tmp/gapView.6539.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00652  [M=270]
Accession:   TIGR00652
Description: DapF: diaminopimelate epimerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                               -----------
   2.1e-107  344.5   0.0   2.3e-107  344.4   0.0    1.0  1  lcl|FitnessBrowser__Koxy:BWI76_RS01200  BWI76_RS01200 diaminopimelate ep


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Koxy:BWI76_RS01200  BWI76_RS01200 diaminopimelate epimerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  344.4   0.0  2.3e-107  2.3e-107       1     269 [.       1     273 [.       1     274 [] 0.97

  Alignments for each domain:
  == domain 1  score: 344.4 bits;  conditional E-value: 2.3e-107
                               TIGR00652   1 meFlkmhGlgNdFvlvdevdeelvkeeaelvrkvcdrhtgvgaDgvllvep.sseeadvklrifNsDGSea 70 
                                             m+F+kmhGlgNdF++vd v+++++++ +el+r+++drh gvg+D++l+vep +++e d+++rifN+DGSe+
  lcl|FitnessBrowser__Koxy:BWI76_RS01200   1 MQFSKMHGLGNDFMVVDAVTQNVFFS-PELIRRLADRHLGVGFDQLLVVEPpYDPELDFHYRIFNADGSEV 70 
                                             89***********************9.***********************999****************** PP

                               TIGR00652  71 emCGNgiRcfakfvyekglkekkelsvetlaglikveveeenkkvkvdmgepkfkkeeipltvekeeekee 141
                                             ++CGNg+Rcfa+fv+ kgl++k++++v+t +g++++ v+e++  v+v+mgep+f+++++p++++k+e+++ 
  lcl|FitnessBrowser__Koxy:BWI76_RS01200  71 AQCGNGARCFARFVRLKGLTNKRDIRVSTSNGRMVLSVTEDD-LVRVNMGEPNFEPSQVPFRANKAEKTYI 140
                                             ******************************************.********************99999888 PP

                               TIGR00652 142 llalev.l...vvdvGnPHlvvfvedvekldleelgklleaheefpegvNvefvevkkedeiklrvyERGa 208
                                             + a+e  +   vv++GnPH+v++v+dve++ +e+lg+++e+he+fpe+ N+ f++v ++++i+lrvyERGa
  lcl|FitnessBrowser__Koxy:BWI76_RS01200 141 MRAAEQtVlcgVVSMGNPHCVIQVDDVETATVETLGPVMENHERFPERANIGFMQVISRSHIRLRVYERGA 211
                                             87777762579************************************************************ PP

                               TIGR00652 209 geTlaCGtGavAsavvalklgktkkkvtvhleggeLeievkedg.kvyltGpavlvlegela 269
                                             geT+aCG+Ga+A+a+v++++g++ ++v+v+l+gg+L i +k  g  +y+tGpa +v++g ++
  lcl|FitnessBrowser__Koxy:BWI76_RS01200 212 GETQACGSGACAAAAVGIQQGLLGEEVRVELPGGRLDIAWKGPGqPLYMTGPAAHVYDGFIH 273
                                             ********************************************99*************875 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (274 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.23
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory