Alignments for a candidate for aroG in Marinobacter adhaerens HP15

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroG in Marinobacter adhaerens HP15

Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate GFF1857 HP15_1814 phospho-2-dehydro-3-heoxyheptonate aldolase

Query= BRENDA::P00888
         (356 letters)



>FitnessBrowser__Marino:GFF1857
          Length = 358

 Score =  416 bits (1070), Expect = e-121
 Identities = 205/355 (57%), Positives = 263/355 (74%), Gaps = 1/355 (0%)

Query: 1   MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKSISDIIAGRDPRLLVVCG 60
           M  + L N+++  ++ L+TPE LK   PLS +    ++  R++I DI+ G+D RL VV G
Sbjct: 1   MSGNKLENLNVASQEALITPEALKKEMPLSDKAAETVSKGRQAIYDIMDGKDHRLFVVVG 60

Query: 61  PCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDV 120
           PCSIHD E A +YA R K LA EVSD+L +VMRVYFEKPRTTVGWKGLINDPH++ +FD+
Sbjct: 61  PCSIHDVEAARDYAARLKKLADEVSDTLLIVMRVYFEKPRTTVGWKGLINDPHLNDTFDI 120

Query: 121 EAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGL 180
           E GL I R+LLL++  +GLP ATEALDP SPQYL D  +WSAIGARTTESQTHREM+SGL
Sbjct: 121 EQGLHIGRRLLLDINELGLPAATEALDPISPQYLQDTIAWSAIGARTTESQTHREMSSGL 180

Query: 181 SMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKA- 239
           SM +GFKNGTDGSL  A+NAM++ + PH F+GI+Q GQVA+++T+GN  GHV+LRGG   
Sbjct: 181 SMAIGFKNGTDGSLDVAVNAMKSVSHPHSFLGIDQQGQVAIIRTKGNNYGHVVLRGGGGK 240

Query: 240 PNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIG 299
           PNY    VA CE+ +++AGLR S+M+DCSH NS+KD   QP V + V  QI +GN SI  
Sbjct: 241 PNYDSVSVALCEQALDKAGLRKSMMIDCSHANSSKDPAIQPLVMQDVTHQILEGNTSIQS 300

Query: 300 LMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHQDLNGQLTAR 354
           LM+ESNI+ GNQS  +  +++KYGVSVTDACI W  T+  +R++   L   L  R
Sbjct: 301 LMVESNINWGNQSIPENLADLKYGVSVTDACIDWPTTEKAIRDMRDKLKDVLPKR 355


Lambda     K      H
   0.316    0.131    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 422
Number of extensions: 10
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 356
Length of database: 358
Length adjustment: 29
Effective length of query: 327
Effective length of database: 329
Effective search space:   107583
Effective search space used:   107583
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate GFF1857 HP15_1814 (phospho-2-dehydro-3-heoxyheptonate aldolase)
to HMM TIGR00034 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00034.hmm
# target sequence database:        /tmp/gapView.15046.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00034  [M=342]
Accession:   TIGR00034
Description: aroFGH: 3-deoxy-7-phosphoheptulonate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                           Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                           -----------
   2.7e-156  505.6   0.1   3.1e-156  505.3   0.1    1.0  1  lcl|FitnessBrowser__Marino:GFF1857  HP15_1814 phospho-2-dehydro-3-he


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Marino:GFF1857  HP15_1814 phospho-2-dehydro-3-heoxyheptonate aldolase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  505.3   0.1  3.1e-156  3.1e-156       2     339 ..       8     349 ..       7     352 .. 0.97

  Alignments for each domain:
  == domain 1  score: 505.3 bits;  conditional E-value: 3.1e-156
                           TIGR00034   2 dlrivkidelltPeelkakfpltekaaekvaksrkeiadilaGkddrllvviGPcsihdpeaaleyakrlkklae 76 
                                         +l++++ + l+tPe+lk+++pl+ kaae+v k r++i di++Gkd+rl+vv+GPcsihd eaa +ya rlkkla+
  lcl|FitnessBrowser__Marino:GFF1857   8 NLNVASQEALITPEALKKEMPLSDKAAETVSKGRQAIYDIMDGKDHRLFVVVGPCSIHDVEAARDYAARLKKLAD 82 
                                         78889999******************************************************************* PP

                           TIGR00034  77 klkddleivmrvyfekPrttvGWkGlindPdlnesfdvnkGlriarkllldlvelglplatelldtispqyladl 151
                                         +++d l ivmrvyfekPrttvGWkGlindP+ln++fd+++Gl+i r+llld++elglp+ate+ld+ispqyl d+
  lcl|FitnessBrowser__Marino:GFF1857  83 EVSDTLLIVMRVYFEKPRTTVGWKGLINDPHLNDTFDIEQGLHIGRRLLLDINELGLPAATEALDPISPQYLQDT 157
                                         *************************************************************************** PP

                           TIGR00034 152 lswgaiGarttesqvhrelasglslpvgfkngtdGslkvaidairaaaaehlflsvtkaGqvaivetkGnedghi 226
                                         ++w+aiGarttesq+hre++sgls+++gfkngtdGsl va++a+++ +++h fl+++++Gqvai++tkGn++gh+
  lcl|FitnessBrowser__Marino:GFF1857 158 IAWSAIGARTTESQTHREMSSGLSMAIGFKNGTDGSLDVAVNAMKSVSHPHSFLGIDQQGQVAIIRTKGNNYGHV 232
                                         *************************************************************************** PP

                           TIGR00034 227 ilrGGkk.pnydaedvaevkeelekaglkeelmidfshgnsnkdykrqlevaesvveqiaeGekaiiGvmiesnl 300
                                         +lrGG   pnyd+ +va +++ l+kagl++++mid+sh+ns+kd   q+ v+++v++qi eG+++i  +m+esn+
  lcl|FitnessBrowser__Marino:GFF1857 233 VLRGGGGkPNYDSVSVALCEQALDKAGLRKSMMIDCSHANSSKDPAIQPLVMQDVTHQILEGNTSIQSLMVESNI 307
                                         ***98766******************************************************************* PP

                           TIGR00034 301 eeGnqsl...keelkyGksvtdacigwedteallrklaeavk 339
                                         + Gnqs+    ++lkyG+svtdaci+w +te+ +r++ +++k
  lcl|FitnessBrowser__Marino:GFF1857 308 NWGNQSIpenLADLKYGVSVTDACIDWPTTEKAIRDMRDKLK 349
                                         *******53234689*********************999876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (342 nodes)
Target sequences:                          1  (358 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 9.54
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis