Alignments for a candidate for aroG in Marinobacter adhaerens HP15

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroG in Marinobacter adhaerens HP15

Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate GFF3314 HP15_3256 phospho-2-dehydro-3-heoxyheptonate aldolase

Query= BRENDA::P00888
         (356 letters)



>FitnessBrowser__Marino:GFF3314
          Length = 350

 Score =  339 bits (870), Expect = 6e-98
 Identities = 170/332 (51%), Positives = 223/332 (67%), Gaps = 4/332 (1%)

Query: 13  DEQVLMTPEQLKAAFPLSLQQEAQIADSRKSISDIIAGRDPRLLVVCGPCSIHDPETALE 72
           +E VL TP +LK   P S     Q+ + R++I  I+ G D R L+V GPCSIHD   ALE
Sbjct: 22  EEVVLPTPAELKLQMPASDDIVRQVDEHRQAIRRILQGSDTRTLIVMGPCSIHDEVAALE 81

Query: 73  YARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLQIARKLLL 132
           Y  + KALA EVSD   +VMR Y EKPRTTVGWKGL+ DP   G+ D+  GL+ +R+LLL
Sbjct: 82  YGEKLKALADEVSDRFLIVMRAYLEKPRTTVGWKGLLYDPERTGAGDLHEGLRRSRRLLL 141

Query: 133 ELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGLSMPVGFKNGTDG 192
            L  MGLPLATEAL P +  YLGDL SW+AIGARTTESQ HRE+ SGL MP GFKNGTDG
Sbjct: 142 NLAAMGLPLATEALSPFAMDYLGDLVSWTAIGARTTESQVHREIVSGLPMPTGFKNGTDG 201

Query: 193 SLATAINAMRAAAQPHRFVGINQAGQVALLQTQGNPDGHVILRGGKA-PNYSPADVAQCE 251
            +A A NAM++A+ PH  +G++  G   ++ T+GNPD H++LRGG+   NY  A + Q  
Sbjct: 202 GIAVATNAMKSASHPHHHLGVSATGAPVMITTRGNPDTHLVLRGGRGITNYDAASIEQAV 261

Query: 252 KEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQ 311
             + +AGL  ++MVDCSH N+ K   RQ  +A+ V+AQ + GN  I GLM+ES +  G Q
Sbjct: 262 GALAEAGLSTAVMVDCSHDNACKQSERQLDIAQDVMAQRRAGNHHIRGLMLESFLEPGRQ 321

Query: 312 SSEQPRSEMKYGVSVTDACISWEMTDALLREI 343
              +   +++YG S+TD C+ W  T+AL+R +
Sbjct: 322 DDGE---DLRYGCSITDPCLGWAQTEALIRSL 350


Lambda     K      H
   0.316    0.131    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 381
Number of extensions: 13
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 356
Length of database: 350
Length adjustment: 29
Effective length of query: 327
Effective length of database: 321
Effective search space:   104967
Effective search space used:   104967
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate GFF3314 HP15_3256 (phospho-2-dehydro-3-heoxyheptonate aldolase)
to HMM TIGR00034 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00034.hmm
# target sequence database:        /tmp/gapView.7679.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00034  [M=342]
Accession:   TIGR00034
Description: aroFGH: 3-deoxy-7-phosphoheptulonate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                           Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                           -----------
   9.1e-132  424.9   0.0   1.1e-131  424.6   0.0    1.0  1  lcl|FitnessBrowser__Marino:GFF3314  HP15_3256 phospho-2-dehydro-3-he


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Marino:GFF3314  HP15_3256 phospho-2-dehydro-3-heoxyheptonate aldolase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  424.6   0.0  1.1e-131  1.1e-131      11     334 ..      26     350 .]      18     350 .] 0.98

  Alignments for each domain:
  == domain 1  score: 424.6 bits;  conditional E-value: 1.1e-131
                           TIGR00034  11 lltPeelkakfpltekaaekvaksrkeiadilaGkddrllvviGPcsihdpeaaleyakrlkklaeklkddleiv 85 
                                         l tP+elk ++p++ +   +v + r++i +il+G+d+r l+v+GPcsihd  aaley ++lk+la++++d++ iv
  lcl|FitnessBrowser__Marino:GFF3314  26 LPTPAELKLQMPASDDIVRQVDEHRQAIRRILQGSDTRTLIVMGPCSIHDEVAALEYGEKLKALADEVSDRFLIV 100
                                         459************************************************************************ PP

                           TIGR00034  86 mrvyfekPrttvGWkGlindPdlnesfdvnkGlriarkllldlvelglplatelldtispqyladllswgaiGar 160
                                         mr+y+ekPrttvGWkGl+ dP+  ++ d+++Glr +r+lll+l+ +glplate+l + +  yl+dl+sw+aiGar
  lcl|FitnessBrowser__Marino:GFF3314 101 MRAYLEKPRTTVGWKGLLYDPERTGAGDLHEGLRRSRRLLLNLAAMGLPLATEALSPFAMDYLGDLVSWTAIGAR 175
                                         *************************************************************************** PP

                           TIGR00034 161 ttesqvhrelasglslpvgfkngtdGslkvaidairaaaaehlflsvtkaGqvaivetkGnedghiilrGGkk.p 234
                                         ttesqvhre+ sgl +p gfkngtdG+++va +a+++a+++h+ l+v+ +G+  +++t+Gn+d+h++lrGG+  +
  lcl|FitnessBrowser__Marino:GFF3314 176 TTESQVHREIVSGLPMPTGFKNGTDGGIAVATNAMKSASHPHHHLGVSATGAPVMITTRGNPDTHLVLRGGRGiT 250
                                         ************************************************************************99* PP

                           TIGR00034 235 nydaedvaevkeelekaglkeelmidfshgnsnkdykrqlevaesvveqiaeGekaiiGvmiesnleeGnqslke 309
                                         nyda++++++   l +agl+  +m+d+sh n+ k+ +rql++a++v++q + G++ i G+m+es+le G+q+ +e
  lcl|FitnessBrowser__Marino:GFF3314 251 NYDAASIEQAVGALAEAGLSTAVMVDCSHDNACKQSERQLDIAQDVMAQRRAGNHHIRGLMLESFLEPGRQDDGE 325
                                         *************************************************************************** PP

                           TIGR00034 310 elkyGksvtdacigwedteallrkl 334
                                         +l yG+s+td c+gw +teal+r l
  lcl|FitnessBrowser__Marino:GFF3314 326 DLRYGCSITDPCLGWAQTEALIRSL 350
                                         **********************976 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (342 nodes)
Target sequences:                          1  (350 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 7.72
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis