Align histidinol dehydrogenase (EC 1.1.1.23) (characterized)
to candidate GFF2484 HP15_2428 histidinol dehydrogenase
Query= BRENDA::Q8G2R2 (430 letters) >FitnessBrowser__Marino:GFF2484 Length = 434 Score = 396 bits (1017), Expect = e-115 Identities = 207/428 (48%), Positives = 280/428 (65%), Gaps = 1/428 (0%) Query: 2 VTTLRQTDPDFEQKFAAFLSGKREVSEDVDRAVREIVDRVRREGDSALLDYSRRFDRIDL 61 +T L + DF A L+ V V+ +VR I+ V+ GD A+L+++ +FDR+ + Sbjct: 5 ITRLNASQSDFNDALAKLLAWDDSVDHQVNESVRHILHEVKTRGDQAVLEFTEKFDRLKV 64 Query: 62 EKTG-IAVTEAEIDAAFDAAPASTVEALKLARDRIEKHHARQLPKDDRYTDALGVELGSR 120 + + ++ + A DA P AL+ A +RI +H RQ K +Y D G LG + Sbjct: 65 GSVAELEMDQSRLQQALDAIPQDQRVALEKAAERIRDYHERQNQKSWQYEDEDGTVLGQK 124 Query: 121 WTAIEAVGLYVPGGTASYPSSVLMNAMPAKVAGVDRIVMVVPAPDGNLNPLVLVAARLAG 180 T ++ GLYVPGG A+YPSSVLMNA+PAKVAGV +VMVVP PDG +N +VL +A +AG Sbjct: 125 VTPLDRAGLYVPGGKAAYPSSVLMNAIPAKVAGVSEVVMVVPTPDGVVNDMVLASAAIAG 184 Query: 181 VSEIYRVGGAQAIAALAYGTETIRPVAKIVGPGNAYVAAAKRIVFGTVGIDMIAGPSEVL 240 V ++ VGGAQA+ ALA+GTETI V KIVGPGN +VA AKR VFG VGIDMIAGPSE+L Sbjct: 185 VDRVFTVGGAQAVGALAFGTETIPAVDKIVGPGNIFVATAKREVFGVVGIDMIAGPSEIL 244 Query: 241 IVADKDNNPDWIAADLLAQAEHDTAAQSILMTNDEAFAHAVEEAVERQLHTLARTETASA 300 +++D +PDWIA DL +QAEHD AQSIL++ D F AVE ++ + L T+ R + Sbjct: 245 VISDGKTDPDWIAMDLFSQAEHDEQAQSILISPDAEFLDAVEASINKLLPTMERADIIRT 304 Query: 301 SWRDFGAVILVKDFEDAIPLANRIAAEHLEIAVADAEAFVPRIRNAGSIFIGGYTPEVIG 360 S D A+I V D + A ++NRIA EHLE++V D EA + IR+AG+IF+G YT E +G Sbjct: 305 SMTDRAALIHVTDLKQAAEVSNRIAPEHLELSVEDPEAMLDDIRHAGAIFMGRYTAEALG 364 Query: 361 DYVGGCNHVLPTARSARFSSGLSVLDYMKRTSLLKLGSEQLRALGPAAIEIARAEGLDAH 420 DY G NHVLPT+ +ARFSS L V D+ KR+S++ + +G A +AR EGL AH Sbjct: 365 DYCAGPNHVLPTSGTARFSSPLGVYDFQKRSSIIGFSAAGADRMGRVASVLARGEGLTAH 424 Query: 421 AQSVAIRL 428 A+S R+ Sbjct: 425 ARSAEYRI 432 Lambda K H 0.318 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 481 Number of extensions: 23 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 430 Length of database: 434 Length adjustment: 32 Effective length of query: 398 Effective length of database: 402 Effective search space: 159996 Effective search space used: 159996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate GFF2484 HP15_2428 (histidinol dehydrogenase)
to HMM TIGR00069 (hisD: histidinol dehydrogenase (EC 1.1.1.23))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00069.hmm # target sequence database: /tmp/gapView.759.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00069 [M=393] Accession: TIGR00069 Description: hisD: histidinol dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.5e-166 538.8 0.6 5.2e-166 538.7 0.6 1.0 1 lcl|FitnessBrowser__Marino:GFF2484 HP15_2428 histidinol dehydrogena Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Marino:GFF2484 HP15_2428 histidinol dehydrogenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 538.7 0.6 5.2e-166 5.2e-166 1 393 [] 37 431 .. 37 431 .. 0.99 Alignments for each domain: == domain 1 score: 538.7 bits; conditional E-value: 5.2e-166 TIGR00069 1 vkeiiedvrkeGdeAlleytekfdkv...kleslrvseeeleealeavdeelkealelaaeniekfhekqlpesv 72 v++i+++v+++Gd+A+le+tekfd++ ++++l++ +++l++al+a++++ + ale+aae+i+++he+q ++s+ lcl|FitnessBrowser__Marino:GFF2484 37 VRHILHEVKTRGDQAVLEFTEKFDRLkvgSVAELEMDQSRLQQALDAIPQDQRVALEKAAERIRDYHERQNQKSW 111 799*********************997777889****************************************** PP TIGR00069 73 eveteegvllgqkvrplervglYvPgGkaaypStvlmtavpAkvAgvkeivvvtPpkkdgkvnpavlaaakllgv 147 ++e+e+g++lgqkv+pl+r+glYvPgGkaaypS+vlm+a+pAkvAgv+e+v+v P+ dg vn+ vla a+++gv lcl|FitnessBrowser__Marino:GFF2484 112 QYEDEDGTVLGQKVTPLDRAGLYVPGGKAAYPSSVLMNAIPAKVAGVSEVVMVVPTP-DGVVNDMVLASAAIAGV 185 ********************************************************6.***************** PP TIGR00069 148 devykvGGaqaiaalayGtetvpkvdkivGPGniyVtaAKklvfgevgidmiaGPsEvlviadesanpelvaaDl 222 d+v++vGGaqa++ala+Gtet+p+vdkivGPGni+V++AK++vfg vgidmiaGPsE+lvi+d +++p+++a+Dl lcl|FitnessBrowser__Marino:GFF2484 186 DRVFTVGGAQAVGALAFGTETIPAVDKIVGPGNIFVATAKREVFGVVGIDMIAGPSEILVISDGKTDPDWIAMDL 260 *************************************************************************** PP TIGR00069 223 lsqaEHdedaqailvttseelaekveeeveeqleelerkeiaekslekngaiilvddleealelsneyApEHLel 297 +sqaEHde+aq+il+++++e++++ve+++++ l ++er++i+++s+++++a+i v dl++a+e+sn++ApEHLel lcl|FitnessBrowser__Marino:GFF2484 261 FSQAEHDEQAQSILISPDAEFLDAVEASINKLLPTMERADIIRTSMTDRAALIHVTDLKQAAEVSNRIAPEHLEL 335 *************************************************************************** PP TIGR00069 298 qtkdpeellkkiknaGsvflGeytpealgdyvaGpnhvLPTsgtArfasglsvedFlkrisvqelskealeelae 372 ++dpe++l++i++aG++f+G+yt+ealgdy+aGpnhvLPTsgtArf+s+l+v+dF+kr+s++ +s ++++++++ lcl|FitnessBrowser__Marino:GFF2484 336 SVEDPEAMLDDIRHAGAIFMGRYTAEALGDYCAGPNHVLPTSGTARFSSPLGVYDFQKRSSIIGFSAAGADRMGR 410 *************************************************************************** PP TIGR00069 373 aveklaeaEgLeaHaeavevR 393 ++ la+ EgL+aHa+++e R lcl|FitnessBrowser__Marino:GFF2484 411 VASVLARGEGLTAHARSAEYR 431 *****************9988 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (393 nodes) Target sequences: 1 (434 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.63 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory