GapMind for Amino acid biosynthesis

 

Alignments for a candidate for ptransferase in Marinobacter adhaerens HP15

Align branched-chain-amino-acid transaminase (EC 2.6.1.42); glutamate-prephenate aminotransferase (EC 2.6.1.79) (characterized)
to candidate GFF448 HP15_436 branched-chain-amino-acid aminotransferase

Query= BRENDA::P54691
         (305 letters)



>FitnessBrowser__Marino:GFF448
          Length = 308

 Score =  169 bits (429), Expect = 6e-47
 Identities = 99/300 (33%), Positives = 165/300 (55%), Gaps = 13/300 (4%)

Query: 7   IAYFEDKFVPFEDAKISVATHALHYGTAAFGGLRGIPDPEDPGTILLFRLDRHGDRLSKS 66
           + + + + VP+ DAK  V TH LHYG   F G+R       P    +FRL  H DRL +S
Sbjct: 9   VIWMDGEMVPWRDAKTHVLTHTLHYGLGCFEGVRAYNTANGPA---IFRLKEHTDRLFRS 65

Query: 67  AKFLHYDI--SAEKIKEVIVDFVKKNQPDKSFYIRPLVYSSGLGIAPRLHNLEKDFLVYG 124
           A  L+  +  S ++I E     V++N  D++ Y+RP+ +    G+  R  NL+   +V  
Sbjct: 66  AHILNMKMPFSKDEINEAQRAAVRENNLDEA-YLRPMAFLGSEGMGLRADNLKVHVMVAA 124

Query: 125 LEMGDYLAAD----GVSCRISSWYRQEDRSFPLRGKISAAYITSALAKTEAVESGFDEAI 180
                Y++ +    G+  R SS+ R        + K +  YI S LA  EA+  G +EA+
Sbjct: 125 WSWPSYMSPEAKEMGIKVRTSSYTRHHVNITMCKAKANGNYINSMLALNEAISGGAEEAL 184

Query: 181 LMNSQGKVCEATGMNVFMVRNGQIVTPGNEQDILEGITRDSILTIAADLGIPTCQRPIDK 240
           L++++G V E +G N+F++R+G + TP      LEGITR +I+  A +L IP  +R I +
Sbjct: 185 LLDNEGYVAEGSGENIFIMRDGVLHTP-ELTSCLEGITRATIMDFARELNIPIKERRITR 243

Query: 241 SELMIADEVFLSGTAAKITPVKRIENFTLGGDR--PITEKLRSVLTAVTENREPKYQDWV 298
            E+ IADE F +GTAA++ P++ ++   +G  +  P+TEKL+++     + +  ++  W+
Sbjct: 244 DEVYIADEAFFTGTAAEVLPIRELDGRVIGAGKRGPVTEKLQAMYFDAVKGKLAEHSGWL 303


Lambda     K      H
   0.320    0.138    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 210
Number of extensions: 12
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 305
Length of database: 308
Length adjustment: 27
Effective length of query: 278
Effective length of database: 281
Effective search space:    78118
Effective search space used:    78118
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory