Alignments for a candidate for aspS2 in Desulfovibrio vulgaris Miyazaki F

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Desulfovibrio vulgaris Miyazaki F

Align aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) (characterized)
to candidate 8501305 DvMF_2038 aspartyl-tRNA synthetase (RefSeq)

Query= reanno::Miya:8501305
         (614 letters)



>FitnessBrowser__Miya:8501305
          Length = 614

 Score = 1236 bits (3199), Expect = 0.0
 Identities = 614/614 (100%), Positives = 614/614 (100%)

Query: 1   MSDQKLDVQQEHQQYIEPLGDWVRSHDCATLTAADTGAEVCLMGWVQYRRDHGGLIFVDL 60
           MSDQKLDVQQEHQQYIEPLGDWVRSHDCATLTAADTGAEVCLMGWVQYRRDHGGLIFVDL
Sbjct: 1   MSDQKLDVQQEHQQYIEPLGDWVRSHDCATLTAADTGAEVCLMGWVQYRRDHGGLIFVDL 60

Query: 61  RDRQGLTQIVFSPDVNPAAHERAHIIRSEYVLAIRGTVRPRPEGMTNPGMKTGEIEVYVS 120
           RDRQGLTQIVFSPDVNPAAHERAHIIRSEYVLAIRGTVRPRPEGMTNPGMKTGEIEVYVS
Sbjct: 61  RDRQGLTQIVFSPDVNPAAHERAHIIRSEYVLAIRGTVRPRPEGMTNPGMKTGEIEVYVS 120

Query: 121 EWKLLNTSKTPAFVIEDRTEASENLRLQYRYLDLRRPRMANNFIVRHKAAQATRRYLDEL 180
           EWKLLNTSKTPAFVIEDRTEASENLRLQYRYLDLRRPRMANNFIVRHKAAQATRRYLDEL
Sbjct: 121 EWKLLNTSKTPAFVIEDRTEASENLRLQYRYLDLRRPRMANNFIVRHKAAQATRRYLDEL 180

Query: 181 GFLEIETPYLTKSTPEGARDFLVPSRLNQGQFYALPQSPQLFKQLLMMSGMERYYQIVRC 240
           GFLEIETPYLTKSTPEGARDFLVPSRLNQGQFYALPQSPQLFKQLLMMSGMERYYQIVRC
Sbjct: 181 GFLEIETPYLTKSTPEGARDFLVPSRLNQGQFYALPQSPQLFKQLLMMSGMERYYQIVRC 240

Query: 241 FRDEDMRADRQLEFTQIDIEMSFVDEEQVMAMAEGLMARVFRDALDREIATPFPRMSYDD 300
           FRDEDMRADRQLEFTQIDIEMSFVDEEQVMAMAEGLMARVFRDALDREIATPFPRMSYDD
Sbjct: 241 FRDEDMRADRQLEFTQIDIEMSFVDEEQVMAMAEGLMARVFRDALDREIATPFPRMSYDD 300

Query: 301 AMARYGVDKPDTRFGLELQDVTHIVRGSNFKLFATAELVKAMRVPGGETMTRKEIDEFTE 360
           AMARYGVDKPDTRFGLELQDVTHIVRGSNFKLFATAELVKAMRVPGGETMTRKEIDEFTE
Sbjct: 301 AMARYGVDKPDTRFGLELQDVTHIVRGSNFKLFATAELVKAMRVPGGETMTRKEIDEFTE 360

Query: 361 FVKIYGAQGLAWIKIRPDEWQSPIAKFLSDEERAGLKDALGLETGDIVFFQAGAPGMVNA 420
           FVKIYGAQGLAWIKIRPDEWQSPIAKFLSDEERAGLKDALGLETGDIVFFQAGAPGMVNA
Sbjct: 361 FVKIYGAQGLAWIKIRPDEWQSPIAKFLSDEERAGLKDALGLETGDIVFFQAGAPGMVNA 420

Query: 421 ALGNLRVRLGEHLGLIDENAFNFLWVTDFPLFEYDEEEKRYVACHHPFTSPKDGHMDVMV 480
           ALGNLRVRLGEHLGLIDENAFNFLWVTDFPLFEYDEEEKRYVACHHPFTSPKDGHMDVMV
Sbjct: 421 ALGNLRVRLGEHLGLIDENAFNFLWVTDFPLFEYDEEEKRYVACHHPFTSPKDGHMDVMV 480

Query: 481 ENPAAARARAYDMVLNGYELGGGSIRNHTAEKQRRMFAALGFSPEEAEAQFGFLTQALEM 540
           ENPAAARARAYDMVLNGYELGGGSIRNHTAEKQRRMFAALGFSPEEAEAQFGFLTQALEM
Sbjct: 481 ENPAAARARAYDMVLNGYELGGGSIRNHTAEKQRRMFAALGFSPEEAEAQFGFLTQALEM 540

Query: 541 GAPPHGGIAFGMDRLAMLLTGSSSIRDVIAFPKTQKATCLLTQAPDSVSARQLRDLGLRL 600
           GAPPHGGIAFGMDRLAMLLTGSSSIRDVIAFPKTQKATCLLTQAPDSVSARQLRDLGLRL
Sbjct: 541 GAPPHGGIAFGMDRLAMLLTGSSSIRDVIAFPKTQKATCLLTQAPDSVSARQLRDLGLRL 600

Query: 601 RETGKDGAPNAPQG 614
           RETGKDGAPNAPQG
Sbjct: 601 RETGKDGAPNAPQG 614


Lambda     K      H
   0.321    0.137    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1428
Number of extensions: 42
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 614
Length of database: 614
Length adjustment: 37
Effective length of query: 577
Effective length of database: 577
Effective search space:   332929
Effective search space used:   332929
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis