GapMind for Amino acid biosynthesis

 

Aligments for a candidate for CBS in Desulfovibrio vulgaris Miyazaki F

Align Putative cystathionine beta-synthase MT1108; EC 4.2.1.22; Beta-thionase; Serine sulfhydrase (uncharacterized)
to candidate 8501944 DvMF_2658 cysteine synthase (RefSeq)

Query= curated2:P9WP50
         (464 letters)



>lcl|FitnessBrowser__Miya:8501944 DvMF_2658 cysteine synthase
           (RefSeq)
          Length = 310

 Score =  234 bits (598), Expect = 2e-66
 Identities = 138/307 (44%), Positives = 183/307 (59%), Gaps = 10/307 (3%)

Query: 1   MRIAQHISELIGGTPLVRLNSVVPDGAGTVAAKVEYLNPGGSSKDRIAVKMIEAAEASGQ 60
           M IA+ ++EL+G TPLVRLN V    A  V AK+E+ NP  S KDRIA+ MI+ A   G+
Sbjct: 1   MEIARDMTELVGRTPLVRLNRVSEGCAAEVVAKLEFNNPCASVKDRIALAMIDGAMERGE 60

Query: 61  LKPGGTIVEPTSGNTGVGLALVAQRRGYKCVFVCPDKVSEDKRNVLIAYGAEVVVCPTAV 120
           L PGG +VEPTSGNTGVGLA VA  RG   V   P+ +S +++ +L  +GA +V+ P + 
Sbjct: 61  LAPGGLLVEPTSGNTGVGLAFVAAVRGLTLVLTMPESMSNERKALLRGFGARLVLTPAS- 119

Query: 121 PPHDPASYYSVSDRLVRDIDGAWKPDQYANPEGPASHYVTTGPEIWADTEGKVTHFVAGI 180
                      ++R+V +  GA    Q+ NP+ P  H  TT  EIWADT+GKV  FVAG+
Sbjct: 120 --KGMRGAIEEAERIVAETPGAVMLGQFVNPDNPLVHRKTTAEEIWADTDGKVDAFVAGV 177

Query: 181 GTGGTITGAGRYLKEVSGGRVRIVGADP-EGSVYSGGAGRPYLVEGVGEDFWPAAYDPSV 239
           GTGGT+TG GR L+E++   +++   +P E  V SGGA  P+ ++G+G  F P   D  V
Sbjct: 178 GTGGTVTGTGRRLRELNPD-IKVFAVEPDESPVLSGGAPGPHPIQGIGAGFVPPVLDTKV 236

Query: 240 PDEIIAVSDSDSFDMTRRLAREEAMLVGGSCGMAVVAALKVA---EEAGPDALIVVLLPD 296
            DEII V   D+    RRL REE +L G S G    AA+ VA   E AG   + VV   D
Sbjct: 237 YDEIIRVPGKDALVTARRLLREEGILCGVSSGANAFAAMAVARRPEMAGKRVVFVVC--D 294

Query: 297 GGRGYMS 303
            G  Y+S
Sbjct: 295 TGERYLS 301


Lambda     K      H
   0.316    0.135    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 457
Number of extensions: 30
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 464
Length of database: 310
Length adjustment: 30
Effective length of query: 434
Effective length of database: 280
Effective search space:   121520
Effective search space used:   121520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory