Align Glutamine amidotransferase of anthranilate synthase (characterized, see rationale)
to candidate 8501008 DvMF_1745 glutamine amidotransferase of anthranilate synthase (RefSeq)
Query= uniprot:B8DM46_DESVM (192 letters) >FitnessBrowser__Miya:8501008 Length = 192 Score = 400 bits (1029), Expect = e-117 Identities = 192/192 (100%), Positives = 192/192 (100%) Query: 1 MFLLIDNYDSFTFNLVQAFYGLGLHPVVVRNDDPAVPAMAEDPALEMVCISPGPSHPRNA 60 MFLLIDNYDSFTFNLVQAFYGLGLHPVVVRNDDPAVPAMAEDPALEMVCISPGPSHPRNA Sbjct: 1 MFLLIDNYDSFTFNLVQAFYGLGLHPVVVRNDDPAVPAMAEDPALEMVCISPGPSHPRNA 60 Query: 61 GFCLDFLSRLPHRVPVLGVCLGHQVLGLFAGATVDVGPRIMHGKTSDITHDGQGLFHGVP 120 GFCLDFLSRLPHRVPVLGVCLGHQVLGLFAGATVDVGPRIMHGKTSDITHDGQGLFHGVP Sbjct: 61 GFCLDFLSRLPHRVPVLGVCLGHQVLGLFAGATVDVGPRIMHGKTSDITHDGQGLFHGVP 120 Query: 121 SPMQVGRYHSLIVHAEERPDLLAVTARAPEGEVMALRYTDRPWVGVQFHPESVLTPDGVR 180 SPMQVGRYHSLIVHAEERPDLLAVTARAPEGEVMALRYTDRPWVGVQFHPESVLTPDGVR Sbjct: 121 SPMQVGRYHSLIVHAEERPDLLAVTARAPEGEVMALRYTDRPWVGVQFHPESVLTPDGVR 180 Query: 181 MLANFPAHVAGR 192 MLANFPAHVAGR Sbjct: 181 MLANFPAHVAGR 192 Lambda K H 0.325 0.142 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 304 Number of extensions: 3 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 192 Length of database: 192 Length adjustment: 20 Effective length of query: 172 Effective length of database: 172 Effective search space: 29584 Effective search space used: 29584 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 44 (21.6 bits)
Align candidate 8501008 DvMF_1745 (glutamine amidotransferase of anthranilate synthase (RefSeq))
to HMM TIGR00566 (glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase)
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00566.hmm # target sequence database: /tmp/gapView.16618.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00566 [M=192] Accession: TIGR00566 Description: trpG_papA: glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.2e-61 193.1 0.0 2.5e-61 192.9 0.0 1.0 1 lcl|FitnessBrowser__Miya:8501008 DvMF_1745 glutamine amidotransfe Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Miya:8501008 DvMF_1745 glutamine amidotransferase of anthranilate synthase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 192.9 0.0 2.5e-61 2.5e-61 1 189 [. 1 185 [. 1 188 [. 0.98 Alignments for each domain: == domain 1 score: 192.9 bits; conditional E-value: 2.5e-61 TIGR00566 1 mvllidnydsftynlvqlleelgaevvvkrndsltlqeieallpllsivisPGPctPdeaaissleliehlaGklPi 77 m llidnydsft+nlvq + lg + vv rnd+ + + + +l ++isPGP++P +a+ l+ + +l ++P+ lcl|FitnessBrowser__Miya:8501008 1 MFLLIDNYDSFTFNLVQAFYGLGLHPVVVRNDDPAVPAMAEDPALEMVCISPGPSHPRNAGFC-LDFLSRLPHRVPV 76 679********************9999999**999*99999999999***************9.************* PP TIGR00566 78 lGvClGhqalaqafGadvvraekvkhGkvseiehngaavfaglfnPdalkatryhslvveaetldtllevtaleeee 154 lGvClGhq l++ Ga v +++hGk+s+i+h+g+++f+g+ +P ++++ryhsl+v+ae+ ++ll vta e lcl|FitnessBrowser__Miya:8501008 77 LGVCLGHQVLGLFAGATVDVGPRIMHGKTSDITHDGQGLFHGVPSP--MQVGRYHSLIVHAEERPDLLAVTARAPEG 151 **********************************************..**************************999 PP TIGR00566 155 ieimairhrdlpleGvqfhPesilselGkellanf 189 e+ma+r d p+ GvqfhPes+l+ G ++lanf lcl|FitnessBrowser__Miya:8501008 152 -EVMALRYTDRPWVGVQFHPESVLTPDGVRMLANF 185 .*********************************9 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (192 nodes) Target sequences: 1 (192 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 8.58 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory