GapMind for Amino acid biosynthesis

 

Aligments for a candidate for argE in Shewanella loihica PV-4

Align acetylornithine deacetylase (EC 3.5.1.16) (characterized)
to candidate 5207679 Shew_0201 acetylornithine deacetylase (RefSeq)

Query= ecocyc::ACETYLORNDEACET-MONOMER
         (383 letters)



>lcl|FitnessBrowser__PV4:5207679 Shew_0201 acetylornithine
           deacetylase (RefSeq)
          Length = 386

 Score =  461 bits (1185), Expect = e-134
 Identities = 219/382 (57%), Positives = 284/382 (74%), Gaps = 1/382 (0%)

Query: 1   MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFKDLGFNVEVQPVPGTRN 60
           M    P   + +R LIATPSISA E  LD SN  ++TLL+ W  DLGF+ ++Q VP TR 
Sbjct: 1   MMKTFPDIKQSFRELIATPSISALEAELDMSNQGVVTLLSQWLTDLGFDCQMQAVPDTRG 60

Query: 61  KFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHDGKLYGLGTADMKGFFAFI 120
           K N+LA IGQG GGLLLAGHTDTVPFD+GRW++DPFTLTE D + YGLG+ DMKGFFA +
Sbjct: 61  KHNLLAKIGQGRGGLLLAGHTDTVPFDEGRWSQDPFTLTEKDNRWYGLGSCDMKGFFALV 120

Query: 121 LDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTALRPDCAIIGEPTSLQPVRAHK 180
           ++A+R +      +PLYILA+ADEET+M GA+ FA++ A+ P+ A+IGEPT L+PV  HK
Sbjct: 121 IEAVRQMPTQDFVRPLYILASADEETTMNGAKAFAQSKAIAPEYALIGEPTGLKPVYMHK 180

Query: 181 GHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQLRDNLKERYHYEAFTVPYPTLN 240
           GH++  IRI G+SGHSSDPA+G+NAIE+MH  IG +L+L+ +L E Y  EAF+VPYPT+N
Sbjct: 181 GHLAQGIRITGRSGHSSDPAKGLNAIEIMHQVIGQLLKLKQHLAEHYREEAFSVPYPTMN 240

Query: 241 LGHIHGGDASNRICACCELHMDIRPLPGMTLNELNGLLNDALAPVSERWPGRLTVDELHP 300
            GHIHGGDA+NRIC CC+LH+DIRPLPG+ L  L  LL   LA +S+R+PG +++ +L+P
Sbjct: 241 FGHIHGGDAANRICGCCDLHLDIRPLPGLPLEVLEQLLTQYLAELSQRYPGSISISQLYP 300

Query: 301 PIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEAPFIQTL-CPTLVLGPGSINQAHQPDE 359
               +    + Q  ++V +L     EVVNY TEAP+IQ L C TLV GPGSI QAHQPDE
Sbjct: 301 GSQPFAGQADAQWSQLVAQLSNQAPEVVNYATEAPYIQQLGCQTLVWGPGSIEQAHQPDE 360

Query: 360 YLETRFIKPTRELITQVIHHFC 381
           YL+T +I  T +L+ Q+I+H C
Sbjct: 361 YLDTAYINKTVDLLKQLIYHAC 382


Lambda     K      H
   0.320    0.139    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 470
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 383
Length of database: 386
Length adjustment: 30
Effective length of query: 353
Effective length of database: 356
Effective search space:   125668
Effective search space used:   125668
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

Align candidate 5207679 Shew_0201 (acetylornithine deacetylase (RefSeq))
to HMM TIGR01892 (argE: acetylornithine deacetylase (ArgE) (EC 3.5.1.16))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01892.hmm
# target sequence database:        /tmp/gapView.15554.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01892  [M=365]
Accession:   TIGR01892
Description: AcOrn-deacetyl: acetylornithine deacetylase (ArgE)
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                        Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                        -----------
     8e-137  442.1   0.0   9.2e-137  441.9   0.0    1.0  1  lcl|FitnessBrowser__PV4:5207679  Shew_0201 acetylornithine deacet


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__PV4:5207679  Shew_0201 acetylornithine deacetylase (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  441.9   0.0  9.2e-137  9.2e-137       3     365 .]      12     378 ..      10     378 .. 0.99

  Alignments for each domain:
  == domain 1  score: 441.9 bits;  conditional E-value: 9.2e-137
                        TIGR01892   3 lakLvafdsvsar......snvdlieyvedyleelgvavevlpvadgaeksnllaviGpkegagglvlsGhtDvvPvd 74 
                                      +++L+a++s+sa+      sn+ +++++ ++l +lg+  + + v+d++ k nlla iG  +g+ggl+l+GhtD+vP+d
  lcl|FitnessBrowser__PV4:5207679  12 FRELIATPSISALeaeldmSNQGVVTLLSQWLTDLGFDCQMQAVPDTRGKHNLLAKIG--QGRGGLLLAGHTDTVPFD 87 
                                      789*********999*******************************************..9***************** PP

                        TIGR01892  75 eaaWtsDpfrLtekdgrLYgrGtaDmkGFlalvLaavpdlaaakLkkPlhlvlsaDeevglaGakkliealarrpala 152
                                      e++W++Dpf+Ltekd+r Yg+G++DmkGF+alv++av+++ +++  +Pl++++saDee+++ Gak +++  a  p+ a
  lcl|FitnessBrowser__PV4:5207679  88 EGRWSQDPFTLTEKDNRWYGLGSCDMKGFFALVIEAVRQMPTQDFVRPLYILASADEETTMNGAKAFAQSKAIAPEYA 165
                                      ****************************************************************************** PP

                        TIGR01892 153 ivGePtslvavRahkGkasakvtvrGreghssrpdrGvsaielaakllarlvaladklkredleeaFtppyatlniGt 230
                                      ++GePt+l++v  hkG+ ++ ++++Gr+ghss+p++G++aie +++++++l++l+++l +++ eeaF +py+t+n G+
  lcl|FitnessBrowser__PV4:5207679 166 LIGEPTGLKPVYMHKGHLAQGIRITGRSGHSSDPAKGLNAIEIMHQVIGQLLKLKQHLAEHYREEAFSVPYPTMNFGH 243
                                      ****************************************************************************** PP

                        TIGR01892 231 vkGGkavniiaaaCelvlelRpipGmdpeellallekiaeevkekapgfevkveelsatpaleleedaelvallekla 308
                                      ++GG+a n+i+++C l+l++Rp+pG+++e l +ll++ ++e++++ pg     ++ +  + ++ ++da+  +l+++l+
  lcl|FitnessBrowser__PV4:5207679 244 IHGGDAANRICGCCDLHLDIRPLPGLPLEVLEQLLTQYLAELSQRYPGSISISQLYPGSQPFAGQADAQWSQLVAQLS 321
                                      ****************************************************************************** PP

                        TIGR01892 309 GaaaevvsygteagllqelGieavvlGPGdidqahqpdeYveieelkrcrallerlv 365
                                       +a+evv+y+tea+++q+lG++++v GPG+i+qahqpdeY+++  ++++ +ll++l+
  lcl|FitnessBrowser__PV4:5207679 322 NQAPEVVNYATEAPYIQQLGCQTLVWGPGSIEQAHQPDEYLDTAYINKTVDLLKQLI 378
                                      *****************************************************9986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (365 nodes)
Target sequences:                          1  (386 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 7.33
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory