Align Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 (characterized)
to candidate 5209622 Shew_2079 aspartyl-tRNA synthetase (RefSeq)
Query= SwissProt::Q51422 (591 letters) >FitnessBrowser__PV4:5209622 Length = 592 Score = 775 bits (2000), Expect = 0.0 Identities = 381/591 (64%), Positives = 464/591 (78%), Gaps = 1/591 (0%) Query: 2 MRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAKA 61 MRSHYCG +N S G+EVTL GWV+R RD GGV+FLD+RDREG+ QVV+DPD E F A Sbjct: 1 MRSHYCGDVNRSHVGEEVTLVGWVNRSRDLGGVVFLDLRDREGVIQVVYDPDLPEVFDVA 60 Query: 62 DRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELEVLNQAETPPFPLDEYSDVGE 121 +RSEF V++ G VR RP+ N M +G IEVLG L +LN A P +D+ E Sbjct: 61 STLRSEFCVQVKGLVRARPDSQINDQMRTGEIEVLGKALTILNSAPALPINMDKNQHNTE 120 Query: 122 ETRLRYRFIDLRRPEMAAKLKLRARITSSIRRYLDDNGFLDVETPILGRPTPEGARDYLV 181 E RL+YR++DLRRPEMA ++ R+++TS++RR+LD NGFLD+ETPIL + TPEGARDYLV Sbjct: 121 EQRLKYRYLDLRRPEMAERIIFRSKVTSAVRRFLDGNGFLDIETPILTKATPEGARDYLV 180 Query: 182 PSRTYPGHFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSF 241 PSRTY G FFALPQSPQLFKQLLM++GFDRYYQI KCFRDEDLRADRQPEFTQIDIETSF Sbjct: 181 PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF 240 Query: 242 LDESDIIGITEKMVRQLFKEVLDVEFDEFPHMPFEEAMRRYGSDKPDLRIPLELVDVADQ 301 + + ++ TE+MVR LFKE+L+V+ EFP M F EAMRRYGSDKPDLR PLELVDVAD Sbjct: 241 MTSAQVMDKTEEMVRGLFKELLNVDLGEFPKMTFAEAMRRYGSDKPDLRNPLELVDVADL 300 Query: 302 LKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNERA 361 +K+V+FKVF PAND +GRVA L VPG AS+ R Q+D+Y K+V IYGAKGLA++KVNE Sbjct: 301 VKDVDFKVFQEPANDSEGRVAVLCVPGGASLSRKQLDEYGKYVNIYGAKGLAWMKVNELE 360 Query: 362 KGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADKAKIVCDALGALRIKVGHDLK 421 G+EG+QSP++KF+ E + IL+R GA +GD++ FGADKA IV +A+GALR+KVG D Sbjct: 361 NGLEGIQSPVLKFLSEEVVKGILERTGAANGDLILFGADKANIVAEAMGALRLKVGEDFD 420 Query: 422 LLTREWAPMWVVDFPMFEENDDGSLSALHHPFTSPK-CTPAELEANPGAALSRAYDMVLN 480 LL +W P+WVVDFPMFE DG L A+HHPFT+P TPAELEA+P AA+S AYDMVLN Sbjct: 421 LLQGDWKPLWVVDFPMFERTSDGGLHAMHHPFTAPSGITPAELEADPTAAISDAYDMVLN 480 Query: 481 GTELGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLV 540 G ELGGGS+RI+D MQ AVFR+LGI++ E +EKFGFLL+ALKYG PPH GLAFGLDR+V Sbjct: 481 GCELGGGSVRIYDAEMQSAVFRILGINDEEAQEKFGFLLEALKYGTPPHAGLAFGLDRMV 540 Query: 541 MLMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLREQPKAE 591 MLMTGASSIR+V+AFPKT +A +T APG + L EL + + E K + Sbjct: 541 MLMTGASSIRDVMAFPKTTTAACPLTNAPGFANPVQLEELGVSVVEAKKEQ 591 Lambda K H 0.321 0.140 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1083 Number of extensions: 40 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 591 Length of database: 592 Length adjustment: 37 Effective length of query: 554 Effective length of database: 555 Effective search space: 307470 Effective search space used: 307470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory