Aligments for a candidate for metY in Shewanella loihica PV-4

GapMind for Amino acid biosynthesis

Aligments for a candidate for metY in Shewanella loihica PV-4

Align homocysteine synthase Met17; EC 2.5.1.49 (characterized)
to candidate 5210267 Shew_2710 O-acetylhomoserine/O-acetylserine sulfhydrylase (RefSeq)

Query= CharProtDB::CH_124469
         (429 letters)



>lcl|FitnessBrowser__PV4:5210267 Shew_2710
           O-acetylhomoserine/O-acetylserine sulfhydrylase (RefSeq)
          Length = 429

 Score =  437 bits (1124), Expect = e-127
 Identities = 225/426 (52%), Positives = 287/426 (67%), Gaps = 6/426 (1%)

Query: 9   ETLQLHAGQEPDAATSSRAVPIYATTSYVFRDCDHGGRLFGLQEPGYIYSRMMNPTADVF 68
           E+L LH G   +A T + AVPIY TTSY F D  HG  LF L+  G IY+R+MNPT DV 
Sbjct: 4   ESLALHHGYTSEATTKAAAVPIYQTTSYTFDDTQHGADLFDLKVAGNIYTRIMNPTTDVL 63

Query: 69  EKRIAALEHGAAAIATSSGTSALFMALTTLAKAGDNIVSTSYLYGGTYNLFKVTLPRLGI 128
           E+R+AA+E G  A+A +SG +A+  A+  L + GDNIVSTS LYGGTYNLF  TLPR G+
Sbjct: 64  EQRLAAIEGGIGALALASGMAAITYAIQALTQVGDNIVSTSQLYGGTYNLFAHTLPRQGV 123

Query: 129 TTKFVNGDDPNDLAAQIDENTKAVYVESIGNPMYNVPDFERIAEVAHAAGVPLMVDNTFG 188
             +    DD + L A ID+ TKA++ ESIGNP  N+ D  R+AE+AH  GVPL+VDNT  
Sbjct: 124 EVRMAAFDDFDGLEAHIDDKTKALFCESIGNPAGNIVDIARLAEIAHRHGVPLIVDNTV- 182

Query: 189 GGGYLVRPIDHGADIVTHSATKWIGGHGTTIGGVIVDSGKFDWKKNSKRFPEFNEPHPGY 248
               L RP +HGADIV HS TK+IGGHGTTIGGVI+DSGKFDW    +RF   NEP P Y
Sbjct: 183 ATPVLCRPFEHGADIVIHSLTKYIGGHGTTIGGVIIDSGKFDWAAQPQRFALLNEPDPSY 242

Query: 249 HGMVFTETFGNLAYAFACRTQTLRDVGGNANPFGVFLLLQGLETLSLRMERHVQNAFALA 308
           HG+V+T+ FG  A+   CR   LR+ G   +P   FLLLQGLETL+LRMERH  NA ALA
Sbjct: 243 HGVVYTQAFGPAAFIGRCRVVPLRNTGAALSPQSAFLLLQGLETLALRMERHCDNALALA 302

Query: 309 KYLEKHPKVNWVSYPGLESHVSHKLAKKYLKNGYGAVLSFGAKGGPDQSRKV-----VNA 363
            +LE+ P+V+WV+Y  L++       +K        ++SFG K    ++ K      ++A
Sbjct: 303 NFLEQDPRVSWVNYGALDNSPYRDNCEKITSGKASGIISFGIKAEDSEAAKAAGGRFIDA 362

Query: 364 LKLASQLANVGDAKTLVIAPAYTTHLQLTDEEQISAGVTKDLIRVAVGIEHIDDIIADFA 423
           L++  +L N+GDAK+L   PA TTH QL  EE   AGV++DLIR++VGIEHI+DIIAD +
Sbjct: 363 LQMILRLVNIGDAKSLACHPATTTHRQLNGEELAKAGVSEDLIRISVGIEHIEDIIADVS 422

Query: 424 QALEVA 429
           QAL+ A
Sbjct: 423 QALDKA 428


Lambda     K      H
   0.318    0.135    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 544
Number of extensions: 21
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 429
Length of database: 429
Length adjustment: 32
Effective length of query: 397
Effective length of database: 397
Effective search space:   157609
Effective search space used:   157609
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis