Align 3-phosphoglycerate dehydrogenase (characterized, see rationale)
to candidate 5208275 Shew_0787 D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding (RefSeq)
Query= uniprot:A0A1X9ZCD3 (315 letters) >FitnessBrowser__PV4:5208275 Length = 329 Score = 124 bits (310), Expect = 4e-33 Identities = 76/210 (36%), Positives = 115/210 (54%), Gaps = 18/210 (8%) Query: 64 KLIGRGGVGMDNIDVEYARSQGINVVNTPAASSLSVAELVFSHLFTGIRFLQDANRKMPV 123 K+I G +N+D+E A+ GI VVN PA S SVAE + + T R + A ++ Sbjct: 70 KIIAMRCAGFNNVDLEAAKRLGIRVVNVPAYSPESVAEHTVALMLTLNRKVHKAYQR--- 126 Query: 124 EGSTQFNNLKKAYAKGTELSGKTIGIIGFGRIGRATAKVALGLGMNVLAYDLYPSESEIT 183 T+ N G + G+T+G+IG G+IG AT K+ LG G V+ YD YP+++ + Sbjct: 127 ---TRDANFSLEGLVGFNMHGRTVGVIGTGKIGVATIKILLGFGCKVVVYDPYPNQAVL- 182 Query: 184 LEFQGGKSVSIPIKTVSLDEVITGSDFFSLHTPFA--DKPILGAEEFAKMKNGVGIVNCS 241 + I+ +SLDE+ SD SLH P + +L F KMK GV ++N S Sbjct: 183 ---------DMGIEYLSLDELYAVSDILSLHCPLTKENHHLLNKASFDKMKPGVMVINTS 233 Query: 242 RGGTIDELALIDALNSGKVSFAGLDVFDNE 271 RGG ++ ++AL +G++ GLDV++NE Sbjct: 234 RGGLLNAFDAMEALKTGQIGSLGLDVYENE 263 Lambda K H 0.316 0.135 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 229 Number of extensions: 10 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 315 Length of database: 329 Length adjustment: 28 Effective length of query: 287 Effective length of database: 301 Effective search space: 86387 Effective search space used: 86387 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory