Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate 5208570 Shew_1081 threonine synthase (RefSeq)
Query= BRENDA::P00934 (428 letters) >FitnessBrowser__PV4:5208570 Length = 427 Score = 494 bits (1272), Expect = e-144 Identities = 254/421 (60%), Positives = 313/421 (74%), Gaps = 2/421 (0%) Query: 1 MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEIDEMLKLDFVTRSAKILS 60 M+LYNLK +++VSF QAV GLGK++GLFFP +P+ L +D +L +DFV RS +L Sbjct: 1 MELYNLKHPSQKVSFTQAVKLGLGKDRGLFFPVSIPK--LDNVDALLAMDFVERSKAVLG 58 Query: 61 AFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGPTLAFKDFGGRFMAQMLTH 120 A++ DE+ QE+++ V AF F P+ + CLELFHGPTLAFKDFG RFMAQ L Sbjct: 59 AWLADELGQEMVDGLVERAFNFELPLTKADDRRFCLELFHGPTLAFKDFGARFMAQCLNS 118 Query: 121 IAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRGKISPLQEKLFCTLGGNIETV 180 +A + +TILTATSGDTGAAVA AFYGL V+VV+LYP+GKIS LQEK+F TLG NI TV Sbjct: 119 LAQEDKLTILTATSGDTGAAVADAFYGLDKVQVVVLYPKGKISLLQEKMFTTLGDNIHTV 178 Query: 181 AIDGDFDACQALVKQAFDDEELKVALGLNSANSINISRLLAQICYYFEAVAQLPQETRNQ 240 A++ DFDACQ +VK AF+D +++ L LNSANSINISRLLAQICYYFEAVAQ Q + Sbjct: 179 AVESDFDACQDMVKAAFEDSDVRDGLHLNSANSINISRLLAQICYYFEAVAQFKQTNQGD 238 Query: 241 LVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVNDTVPRFLHDGQWSPKATQATLSNA 300 V++VPSGNFG+LTAGL AK++GLPVKRF+AATN NDTVPR+L DGQWSPKAT ATLSNA Sbjct: 239 PVIAVPSGNFGNLTAGLFAKAMGLPVKRFVAATNSNDTVPRYLADGQWSPKATVATLSNA 298 Query: 301 MDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDETTQQTMRELKELGYTSEPHAAVAYRA 360 MDV++P+NWPRVE + W L EL + ++ T + + EL GY SEPHAA+A +A Sbjct: 299 MDVAEPSNWPRVETIMEVMNWPLSELVGVGLSEQDTVEALDELYCGGYLSEPHAAIAAKA 358 Query: 361 LRDQLNPGEYGLFLGTAHPAKFKESVEAILGETLDLPKELAERADLPLLSHNLPADFAAL 420 L ++ E G+FLGTAHPAKFKE VE L L+LP EL D P+LS LPADFA L Sbjct: 359 LSLTMSADEQGIFLGTAHPAKFKEVVEQALAIELELPPELQAVKDKPILSAELPADFARL 418 Query: 421 R 421 + Sbjct: 419 K 419 Lambda K H 0.319 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 520 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 428 Length of database: 427 Length adjustment: 32 Effective length of query: 396 Effective length of database: 395 Effective search space: 156420 Effective search space used: 156420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate 5208570 Shew_1081 (threonine synthase (RefSeq))
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00260.hmm # target sequence database: /tmp/gapView.1764.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00260 [M=340] Accession: TIGR00260 Description: thrC: threonine synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.7e-102 329.0 0.0 2e-102 328.8 0.0 1.0 1 lcl|FitnessBrowser__PV4:5208570 Shew_1081 threonine synthase (Re Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__PV4:5208570 Shew_1081 threonine synthase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 328.8 0.0 2e-102 2e-102 27 331 .. 79 388 .. 50 395 .. 0.91 Alignments for each domain: == domain 1 score: 328.8 bits; conditional E-value: 2e-102 TIGR00260 27 rspklaeevgaenlyvkelfhgPtlaFKDrglqfvavlltkalelgnetvlcAtsGdtgaaaaealagkanvkvvvLy 104 +++ + + + +++elfhgPtlaFKD+g +f+a+ l+ ++++ + t+l+AtsGdtgaa+a a++g v+vvvLy lcl|FitnessBrowser__PV4:5208570 79 NFELPLTKADD-RRFCLELFHGPTLAFKDFGARFMAQCLNSLAQEDKLTILTATSGDTGAAVADAFYGLDKVQVVVLY 155 55444444444.779*************************************************************** PP TIGR00260 105 PkgkispvkeklvtalaenakvlaikGdFDdaqdlvkeifedke...klklnsvNsinparieaqktyafeiveqlgk 179 Pkgkis+++ek++t+l n++++a++ dFD++qd+vk++fed+ l lns+Nsin++r++aq++y+fe+v+q ++ lcl|FitnessBrowser__PV4:5208570 156 PKGKISLLQEKMFTTLGDNIHTVAVESDFDACQDMVKAAFEDSDvrdGLHLNSANSINISRLLAQICYYFEAVAQFKQ 233 ******99*********************************7777789****************************** PP TIGR00260 180 espdkvvvpvpsgnfgailkGflekkelglpieklaiaaegaadivrrflksgdlepkedkeTlstAmdignpsnver 257 ++ v++vpsgnfg++++G ++k ++ lp k+++aa++++d+v+r+l +g+++pk++++Tls+Amd+ +psn++r lcl|FitnessBrowser__PV4:5208570 234 TNQGDPVIAVPSGNFGNLTAGLFAKAMG-LP-VKRFVAATNSNDTVPRYLADGQWSPKATVATLSNAMDVAEPSNWPR 309 999999***************9999998.**.88899988889*********************************** PP TIGR00260 258 alelarrslgnledl.kesvsdeeileaikklaeeegyllephtavavaalkklvekg.vs...atadpaKFeevve. 329 ++ +++ ++ l +l + ++s+++++ea +l+ gyl eph+a+a +al ++ ++ ++ +ta+paKF+evve lcl|FitnessBrowser__PV4:5208570 310 VETIMEVMNWPLSELvGVGLSEQDTVEALDELYC-GGYLSEPHAAIAAKALSLTMSADeQGiflGTAHPAKFKEVVEq 386 **********9988626699***********996.7*******************999766889************95 PP TIGR00260 330 al 331 al lcl|FitnessBrowser__PV4:5208570 387 AL 388 54 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (340 nodes) Target sequences: 1 (427 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 10.60 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory