GapMind for Amino acid biosynthesis

 

Aligments for a candidate for argD in Pedobacter sp. GW460-11-11-14-LB5

Align Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 (uncharacterized)
to candidate CA265_RS07530 CA265_RS07530 adenosylmethionine--8-amino-7-oxononanoate transaminase

Query= curated2:P59319
         (406 letters)



>lcl|FitnessBrowser__Pedo557:CA265_RS07530 CA265_RS07530
           adenosylmethionine--8-amino-7-oxononanoate transaminase
          Length = 433

 Score =  158 bits (399), Expect = 3e-43
 Identities = 114/382 (29%), Positives = 186/382 (48%), Gaps = 22/382 (5%)

Query: 29  VRGEGSRVWDQSGRELIDFAGGIAVNALGHCHPALVKALTEQANTLWHVSNV-FTNEPAL 87
           VRGEG  V+D+SG+  ID      VN  GH HP + + + EQ N L HV    FT+EPA+
Sbjct: 41  VRGEGVYVFDESGKRYIDAVSSWWVNIHGHSHPYIAQKVAEQLNVLEHVIFAGFTHEPAV 100

Query: 88  RLAHKLVDATFA--DRAFFCNSGAESNEAAFKLARRVAHDRFGPQKHEIIATVNSFHGRT 145
            LA +L+       D+ F+ ++G+ + E A K+  +   D  G  K +I+A  N++HG T
Sbjct: 101 LLAERLLPILPGKQDKVFYTDNGSTAVEVALKMCLQY-WDNKGTPKTKILAFKNAYHGDT 159

Query: 146 LFTVSVGGQPKYSDGFGPKITGISHVP----YN--DLEALKAQISDKTCAVVIEPIQGES 199
              +SV G+  ++D F   +  +  +     YN   L +  + +SD  C +    I G  
Sbjct: 160 FGAMSVSGRSIFTDAFNSLLFDVEFIDLPNEYNISHLTSHLSNLSDTACFIFEPLILGSG 219

Query: 200 GVVPADKAYLEGARKLCDEHNALLIFDEVQTGVGRTGSLYAYQHYGVIPDILTSAKSL-G 258
           G++  +  YL+     C E   L I DEV TG GRTG+ +A +     PDI+  +K L G
Sbjct: 220 GMLMYEAKYLDQLLSACKEAGILTIADEVMTGFGRTGTYFACEKLINKPDIICLSKGLTG 279

Query: 259 GGFPIGAMLTTTELAK------HLAVGTHGTTYGGNPLGCAVACAVLDVVNTPETLAGIK 312
           G  P+G    T E+ +       L    HG ++  NP+ C  + A LD++   ETL  IK
Sbjct: 280 GTMPLGVTTCTNEIFEAFLSDDKLKTLYHGHSFTANPIACVASLASLDILLKSETLQNIK 339

Query: 313 AKHERFKTRLEQIGQQYNLFSQVRGVGLLLGCVL----TEAWKGKAKDVLNAAEKEGVMV 368
               +    LE+I + +     +R  G ++          ++    +++L A   +  ++
Sbjct: 340 RVEAKHAVFLEEI-KTHPKVKAIRQTGTIIAIEWETGNETSYLSNLRNLLYAYFLDKGII 398

Query: 369 LQAGPDVVRFAPSLVVEDADID 390
           L+   +++   P  V+ D D+D
Sbjct: 399 LRPLGNIIYILPPYVISDEDLD 420


Lambda     K      H
   0.319    0.136    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 403
Number of extensions: 18
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 406
Length of database: 433
Length adjustment: 32
Effective length of query: 374
Effective length of database: 401
Effective search space:   149974
Effective search space used:   149974
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory