GapMind for Amino acid biosynthesis

 

Aligments for a candidate for hisA in Pedobacter sp. GW460-11-11-14-LB5

Align 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; EC 5.3.1.16; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (uncharacterized)
to candidate CA265_RS19260 CA265_RS19260 imidazole glycerol phosphate synthase subunit HisF

Query= curated2:Q46LD2
         (256 letters)



>lcl|FitnessBrowser__Pedo557:CA265_RS19260 CA265_RS19260 imidazole
           glycerol phosphate synthase subunit HisF
          Length = 255

 Score = 92.0 bits (227), Expect = 1e-23
 Identities = 67/231 (29%), Positives = 113/231 (48%), Gaps = 21/231 (9%)

Query: 3   IIPAIDLLNGKCVRLNQGNYNEVTKFNS-----DPVKQAEIWESKGAKRLHLVDLDGAKT 57
           +IPA+ LLN        G   + TKF +     DP+    I+ +K    L ++D+D +K 
Sbjct: 6   VIPAL-LLN------QHGGLVKTTKFAAPKYIGDPLNAMRIFNTKEVDELMVLDIDASKL 58

Query: 58  GEPINDLTIKEIKKSITIPIQLGGGIRSIDRAKELFDIGIDRIILGTIAIEKPELVKDLS 117
               N   I++      +P+  GGGI++ID+A ++F +G+++I L T  +E  +LV+DL 
Sbjct: 59  KREPNYGLIEQFAGECFMPLCYGGGIKTIDQAYKIFKLGVEKICLQTAVLEDLDLVRDLV 118

Query: 118 KEY-PKRIAVGIDAKEGMVATRGWLKQSEISS-----LDLAKQLNDLDLAAIISTDIATD 171
             +    I V +D K+  +      K SE  +     +   KQ  D     I+   +  D
Sbjct: 119 ARFGSSAIVVSVDVKKDWLQRPKLFKSSEGKNANENWISYIKQAVDAGAGEILLNAVDKD 178

Query: 172 GTLKGPNVQALREIAEISINPVIASGGIGSIADLISLADFADEGIEGIIVG 222
           GTL+G ++  +++ +     P+IA GGIGS+ D   + D  D G   +  G
Sbjct: 179 GTLQGSDLTLIKQASGEISAPLIALGGIGSLKD---IKDAVDAGASAVAAG 226



 Score = 31.6 bits (70), Expect = 2e-05
 Identities = 25/76 (32%), Positives = 36/76 (47%), Gaps = 5/76 (6%)

Query: 33  VKQAEIWESKGAKRLHLVDLDGAKTGEPINDLT-IKEIKKSITIPIQLGGGIRSIDRAKE 91
           +KQA +    G   L+ VD DG   G   +DLT IK+    I+ P+   GGI S+   K+
Sbjct: 159 IKQA-VDAGAGEILLNAVDKDGTLQG---SDLTLIKQASGEISAPLIALGGIGSLKDIKD 214

Query: 92  LFDIGIDRIILGTIAI 107
             D G   +  G   +
Sbjct: 215 AVDAGASAVAAGAFFV 230


Lambda     K      H
   0.316    0.138    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 192
Number of extensions: 12
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 256
Length of database: 255
Length adjustment: 24
Effective length of query: 232
Effective length of database: 231
Effective search space:    53592
Effective search space used:    53592
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory