GapMind for Amino acid biosynthesis

 

Aligments for a candidate for dapX in Pedobacter sp. GW460-11-11-14-LB5

Align Probable N-acetyl-LL-diaminopimelate aminotransferase; Putative aminotransferase A; EC 2.6.1.- (characterized)
to candidate CA265_RS07515 CA265_RS07515 pyridoxal phosphate-dependent aminotransferase

Query= SwissProt::P16524
         (393 letters)



>lcl|FitnessBrowser__Pedo557:CA265_RS07515 CA265_RS07515 pyridoxal
           phosphate-dependent aminotransferase
          Length = 380

 Score =  250 bits (639), Expect = 4e-71
 Identities = 145/369 (39%), Positives = 218/369 (59%), Gaps = 18/369 (4%)

Query: 23  LVAQHEDVISLTIGQPDFFTPHHVKAAAKKAIDENVTSYTPNAGYLELRQAVQLYMKKKA 82
           L ++  ++ISL++G+PDF TP HVK AAKKA+DEN T Y+P  GY +LRQA+   +K + 
Sbjct: 8   LASKGINIISLSVGEPDFNTPDHVKNAAKKALDENYTRYSPVPGYPDLRQAIVNKLKTEN 67

Query: 83  DFNYDAESEIIITTGASQAIDAAFRTILSPGDEVIMPGPIYPGYEPIINLCGAKPVIVDT 142
           + +YD  S+I+++TGA Q++     T++ P DEVI+P P +  Y  ++ L   K V +DT
Sbjct: 68  NLDYDI-SQIVVSTGAKQSLSNVILTLIDPDDEVIIPTPYWVSYSEMVTLAEGKSVFIDT 126

Query: 143 -TSHGFKLTARLIEDALTPNTKCVVLPYPSNPTGVTLSEEELKSIAALL-KGRNVFVLSD 200
                FK+T   +E A+TP +K  +   P NPTG   S+EEL ++ A+  K  N+++LSD
Sbjct: 127 DIESDFKITPAQLEAAITPKSKLFMFSSPCNPTGSVYSKEELAALVAVFEKHPNIYILSD 186

Query: 201 EIYSELTYDRPHYSIATY--LRDQTIVINGLSKSHSMTGWRIGFLFAPKDIAKHILKVHQ 258
           EIY  + +   H SIA +  ++D+ I++NG SK+ +MTGWR+G++ A K+IA    K+  
Sbjct: 187 EIYEHINFVDKHESIAQFDSIKDRVIIVNGFSKAFAMTGWRLGYIAANKEIAAANDKLQG 246

Query: 259 YNVSCASSISQKAALEAVTNGFDDALIMREQYKKRLDYVYDRLVSM-GLDVVKPSGAFYI 317
              S   SI+Q+A + A   G    L M+E + +R + VY+ L  + G+    P GAFY 
Sbjct: 247 QTTSGTCSIAQRAGIVAYEQGLASVLEMKEAFLRRRELVYNLLNEIPGVKTNLPDGAFYF 306

Query: 318 FPSIKSFG----------MTSFDFSMALLEDAGVALVPGSSFSTYGEGYVRLSFACSMDT 367
           FP I SF             S D ++ LL    VA V G SF      Y+RLS+A S ++
Sbjct: 307 FPEISSFFGKKDADGNVIKDSSDLALYLLNVGHVATVGGDSFG--NNNYIRLSYAASDES 364

Query: 368 LREGLDRLE 376
           L E L R++
Sbjct: 365 LVEALRRIK 373


Lambda     K      H
   0.319    0.135    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 346
Number of extensions: 17
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 393
Length of database: 380
Length adjustment: 30
Effective length of query: 363
Effective length of database: 350
Effective search space:   127050
Effective search space used:   127050
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory