Alignments for a candidate for argI in Pseudomonas putida KT2440

GapMind for Amino acid biosynthesis

Alignments for a candidate for argI in Pseudomonas putida KT2440

Align Ornithine carbamoyltransferase, catabolic; OTCase; EC 2.1.3.3 (characterized)
to candidate PP_1000 PP_1000 ornithine carbamoyltransferase, catabolic

Query= SwissProt::P08308
         (336 letters)



>FitnessBrowser__Putida:PP_1000
          Length = 336

 Score =  634 bits (1634), Expect = 0.0
 Identities = 309/336 (91%), Positives = 325/336 (96%)

Query: 1   MAFNMHNRNLLSLMHHSTRELRYLLDLSRDLKRAKYTGTEQQHLKRKNIALIFEKTSTRT 60
           MAFN+HNRNLLSL HH+TRELRYLLDLSRDLKRAKYTGTEQQHLK  NIALIFEKTSTRT
Sbjct: 1   MAFNIHNRNLLSLEHHTTRELRYLLDLSRDLKRAKYTGTEQQHLKGNNIALIFEKTSTRT 60

Query: 61  RCAFEVAAYDQGANVTYIDPNSSQIGHKESMKDTARVLGRMYDAIEYRGFKQEIVEELAK 120
           RCAFEVAAYDQGANVTYIDPNSSQIGHKESMKDTARVLGRMYDAIEYRGFKQEIVEELAK
Sbjct: 61  RCAFEVAAYDQGANVTYIDPNSSQIGHKESMKDTARVLGRMYDAIEYRGFKQEIVEELAK 120

Query: 121 FAGVPVFNGLTDEYHPTQMLADVLTMREHSDKPLHDISYAYLGDARNNMGNSLLLIGAKL 180
           FAGVPVFNGLTDEYHPTQM+ADVLTMREHSDKPLHDISYAYLGDARNNMGNSLLLIGAKL
Sbjct: 121 FAGVPVFNGLTDEYHPTQMIADVLTMREHSDKPLHDISYAYLGDARNNMGNSLLLIGAKL 180

Query: 181 GMDVRIAAPKALWPHDEFVAQCKKFAEESGAKLTLTEDPKEAVKGVDFVHTDVWVSMGEP 240
           GMDVRIAAPKALWPHD+ V +CK++AEESGA++TLTEDPK AVKGVDFVHTDVWVSMGEP
Sbjct: 181 GMDVRIAAPKALWPHDDLVERCKQYAEESGARITLTEDPKAAVKGVDFVHTDVWVSMGEP 240

Query: 241 VEAWGERIKELLPYQVNMEIMKATGNPRAKFMHCLPAFHNSETKVGKQIAEQYPNLANGI 300
           +EAWGERIK+L PYQVN E+MK+TGNPR KFMHCLPAFHNSETKVGKQIAEQYP+LANGI
Sbjct: 241 IEAWGERIKQLKPYQVNAELMKSTGNPRTKFMHCLPAFHNSETKVGKQIAEQYPDLANGI 300

Query: 301 EVTEDVFESPYNIAFEQAENRMHTIKAILVSTLADI 336
           EVT+DVFESP  IAFEQAENRMHTIKAILVSTLAD+
Sbjct: 301 EVTDDVFESPACIAFEQAENRMHTIKAILVSTLADL 336


Lambda     K      H
   0.318    0.133    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 500
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 336
Length of database: 336
Length adjustment: 28
Effective length of query: 308
Effective length of database: 308
Effective search space:    94864
Effective search space used:    94864
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate PP_1000 PP_1000 (ornithine carbamoyltransferase, catabolic)
to HMM TIGR00658 (argF: ornithine carbamoyltransferase (EC 2.1.3.3))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00658.hmm
# target sequence database:        /tmp/gapView.23395.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00658  [M=304]
Accession:   TIGR00658
Description: orni_carb_tr: ornithine carbamoyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                           Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                           -----------
   2.8e-138  446.2   0.0   3.2e-138  446.0   0.0    1.0  1  lcl|FitnessBrowser__Putida:PP_1000  PP_1000 ornithine carbamoyltrans


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Putida:PP_1000  PP_1000 ornithine carbamoyltransferase, catabolic
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  446.0   0.0  3.2e-138  3.2e-138       1     303 [.       8     333 ..       8     334 .. 0.99

  Alignments for each domain:
  == domain 1  score: 446.0 bits;  conditional E-value: 3.2e-138
                           TIGR00658   1 rhllslldlseeelkellelakklkkekkkgkeekklkgktlaliFekrstRtRvsfevaayelGaqvlylnkee 75 
                                         r+llsl +++++el++ll+l+++lk++k++g+e+++lkg+++aliFek+stRtR++fevaay++Ga+v+y+++++
  lcl|FitnessBrowser__Putida:PP_1000   8 RNLLSLEHHTTRELRYLLDLSRDLKRAKYTGTEQQHLKGNNIALIFEKTSTRTRCAFEVAAYDQGANVTYIDPNS 82 
                                         78************************************************************************* PP

                           TIGR00658  76 lqlgrkesikDtarvlsryvdaivvRvykhedveelakyasvPvingLtdlehPcqilaDlltikeklg.klkev 149
                                         +q+g+kes+kDtarvl+r++dai +R++k+e+veelak+a+vPv+ngLtd+ hP+q++aD+lt++e+ +  l+++
  lcl|FitnessBrowser__Putida:PP_1000  83 SQIGHKESMKDTARVLGRMYDAIEYRGFKQEIVEELAKFAGVPVFNGLTDEYHPTQMIADVLTMREHSDkPLHDI 157
                                         *********************************************************************99**** PP

                           TIGR00658 150 klvyvGDa.nnvanslllaaaklGldvvvatPeglepeaeivkkakkiakenggkleltedpkkavkdadviytD 223
                                         +++y+GDa nn++nslll++aklG+dv++a+P++l+p+ ++v+++k+ a+e+g++++ltedpk avk++d+++tD
  lcl|FitnessBrowser__Putida:PP_1000 158 SYAYLGDArNNMGNSLLLIGAKLGMDVRIAAPKALWPHDDLVERCKQYAEESGARITLTEDPKAAVKGVDFVHTD 232
                                         *************************************************************************** PP

                           TIGR00658 224 vwvsmGe.eekkeerlkllkpyqvneellela.kpevkflhCLPavr...................Geevtdevl 277
                                         vwvsmGe  e++ er+k+lkpyqvn el++ + +p +kf+hCLPa++                   G evtd+v+
  lcl|FitnessBrowser__Putida:PP_1000 233 VWVSMGEpIEAWGERIKQLKPYQVNAELMKSTgNPRTKFMHCLPAFHnsetkvgkqiaeqypdlanGIEVTDDVF 307
                                         *******8899*********************9****************************************** PP

                           TIGR00658 278 egeasivfdeaenRlhaqkavlkall 303
                                         e++a i f++aenR+h++ka+l+ +l
  lcl|FitnessBrowser__Putida:PP_1000 308 ESPACIAFEQAENRMHTIKAILVSTL 333
                                         **********************9887 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (304 nodes)
Target sequences:                          1  (336 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 9.82
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis