Align Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.- (characterized)
to candidate PP_0930 PP_0930 Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Query= SwissProt::Q9RF06 (475 letters) >FitnessBrowser__Putida:PP_0930 Length = 481 Score = 460 bits (1183), Expect = e-134 Identities = 231/480 (48%), Positives = 328/480 (68%), Gaps = 7/480 (1%) Query: 1 MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAM 60 M +E VIGLE+H +L T SK+FS S FG+EPN+ +++DL PGVLPV+N++AV A Sbjct: 1 MQWEVVIGLEIHTQLATQSKIFSGSATTFGSEPNTQASLVDLGMPGVLPVLNEQAVRMAC 60 Query: 61 RAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEV-DGETKRIGIT 119 +A++ EI + F RKNYFYPD PK YQISQ D PI G++DI + DG KRIG+T Sbjct: 61 MFGLAIDAEIGKRNVFARKNYFYPDLPKGYQISQMDLPIVGKGHLDIALEDGTIKRIGVT 120 Query: 120 RLHMEEDAGKSTHK--GEYSLVDLNRQGTPLIEIVSEPDIRSPKEAYAYLEKLRSIIQYT 177 R H+EEDAGKS H+ + +DLNR GTPL+EIVSEPD+RS KEA AY++ + ++++Y Sbjct: 121 RAHLEEDAGKSLHEDFSGSTGIDLNRAGTPLLEIVSEPDMRSAKEAVAYVKAIHALVRYL 180 Query: 178 GVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLS 237 G+ D M EGSLRCD N+S+RP GQ +FGT+ E+KN+NSF ++ + + E +RQ + + Sbjct: 181 GICDGNMAEGSLRCDCNVSIRPKGQTEFGTRCEIKNVNSFRFIERAINSEIQRQIDLIED 240 Query: 238 GGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKERVRQTIPELPD 297 GG++ QETR +D + +T MR KE ++DYRYFP+PD++P+ I+D++ E +R +PELP Sbjct: 241 GGKVVQETRLYDPNKDETRSMRSKEEANDYRYFPDPDLLPVVIEDSFLETIRAGLPELPP 300 Query: 298 ERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQV 357 ++ ++ + GL AYDA+VL ++E +D+FE ++ G D KL +NW+M + LNK V Sbjct: 301 QKVERFQTQYGLSAYDANVLASSREQADYFEEVVKIGGDAKLAANWVMVELGSLLNKLGV 360 Query: 358 ELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGLVQISDEAT 417 E+ ++ L GM+ I D T+S KIAK VF +AA G+A I+E GL Q++D Sbjct: 361 EIDQAPVSAAQLGGMLLRIRDNTISGKIAKTVFEAMAAGEGDADSIIESKGLKQVTDTGA 420 Query: 418 LLKFVNEALDNNEQSVEDYKNG----KGKAMGFLVGQIMKASKGQANPQLVNQLLKQELD 473 + K ++E L N + VE Y+ +GK GF VGQ MKASKG+ANP VNQLLK +L+ Sbjct: 421 IDKMLDEMLAANAEQVEQYRAADEAKRGKMFGFFVGQAMKASKGKANPGQVNQLLKAKLE 480 Lambda K H 0.313 0.133 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 641 Number of extensions: 28 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 475 Length of database: 481 Length adjustment: 34 Effective length of query: 441 Effective length of database: 447 Effective search space: 197127 Effective search space used: 197127 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 51 (24.3 bits)
Align candidate PP_0930 PP_0930 (Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00133.hmm # target sequence database: /tmp/gapView.26307.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00133 [M=481] Accession: TIGR00133 Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.5e-173 562.1 0.0 5.1e-173 561.9 0.0 1.0 1 lcl|FitnessBrowser__Putida:PP_0930 PP_0930 Aspartyl/glutamyl-tRNA(A Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Putida:PP_0930 PP_0930 Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 561.9 0.0 5.1e-173 5.1e-173 2 480 .. 1 479 [. 1 480 [. 0.98 Alignments for each domain: == domain 1 score: 561.9 bits; conditional E-value: 5.1e-173 TIGR00133 2 ieyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalnskivsev 76 +++e+viGlE+H+ql t+sK+F+ + + + + +pNt+ + v+lg+PG+lPvlN++av++A + +la++++i + lcl|FitnessBrowser__Putida:PP_0930 1 MQWEVVIGLEIHTQLATQSKIFSGSATTFGS-EPNTQASLVDLGMPGVLPVLNEQAVRMACMFGLAIDAEIG-KR 73 5789***************************.**************************************55.69 PP TIGR00133 77 svFdRKhYfYpDlPkgyqitqqdlPiaedGkleieleeke.keigierlhlEeDtgksqykesdkdkqslvDfNR 150 vF+RK+YfYpDlPkgyqi+q dlPi+ +G+l+i le++ k+ig++r hlEeD+gks ++ + + +D+NR lcl|FitnessBrowser__Putida:PP_0930 74 NVFARKNYFYPDLPKGYQISQMDLPIVGKGHLDIALEDGTiKRIGVTRAHLEEDAGKSLHEDF--SGSTGIDLNR 146 9***********************************99888*******************954..67999***** PP TIGR00133 151 sgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlkGqekegtrvEiKNlnslk 225 +g+PLlEiV++Pd++sakea+a++k +++++ryl+i dg++ eGs+R+D+Nvsir+kGq+++gtr EiKN+ns++ lcl|FitnessBrowser__Putida:PP_0930 147 AGTPLLEIVSEPDMRSAKEAVAYVKAIHALVRYLGICDGNMAEGSLRCDCNVSIRPKGQTEFGTRCEIKNVNSFR 221 *************************************************************************** PP TIGR00133 226 siekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRYfpePdlppieideevvkekveekl 300 ie+ai+ Ei+Rq++l+++g +v+qetr +d +k t s+R+Kee++DYRYfp+Pdl p++i+++++++ +++ l lcl|FitnessBrowser__Putida:PP_0930 222 FIERAINSEIQRQIDLIEDGGKVVQETRLYDPNKDETRSMRSKEEANDYRYFPDPDLLPVVIEDSFLET-IRAGL 295 *****************************************************************9988.***** PP TIGR00133 301 pelPeakrirlkkeyglseedakvlvsdlelldafeevvklikepklavnWileellgeLnkkkislaeallkpe 375 pelP +k +r++++ygls++da+vl s +e +d+feevvk +++kla+nW++ el + Lnk +++ +a +++ lcl|FitnessBrowser__Putida:PP_0930 296 PELPPQKVERFQTQYGLSAYDANVLASSREQADYFEEVVKIGGDAKLAANWVMVELGSLLNKLGVEIDQAPVSAA 370 *************************************************************************** PP TIGR00133 376 elaeliklikegkisqksakelleellenkkdpkklieklgliqisdekelvkiveevikenpkevekyk....s 446 +l ++ i++++is+k+ak+++e + ++d++++ie +gl q++d+ ++ k+++e+++ n+++ve+y+ + lcl|FitnessBrowser__Putida:PP_0930 371 QLGGMLLRIRDNTISGKIAKTVFEAMAAGEGDADSIIESKGLKQVTDTGAIDKMLDEMLAANAEQVEQYRaadeA 445 *********************************************************************988877 PP TIGR00133 447 gkekalkflvGqvmkktkgradpkevekllkell 480 ++ k+++f+vGq+mk +kg+a+p +v++llk +l lcl|FitnessBrowser__Putida:PP_0930 446 KRGKMFGFFVGQAMKASKGKANPGQVNQLLKAKL 479 8899***************************988 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (481 nodes) Target sequences: 1 (481 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 11.45 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory