Align N-succinyldiaminopimelate-aminotransferase (EC 2.6.1.17) (characterized)
to candidate PP_1588 PP_1588 N-succinyl-L,L-diaminopimelate aminotransferase alternative
Query= metacyc::MONOMER-6501 (397 letters) >FitnessBrowser__Putida:PP_1588 Length = 398 Score = 432 bits (1112), Expect = e-126 Identities = 227/395 (57%), Positives = 269/395 (68%), Gaps = 4/395 (1%) Query: 1 MNPRLDALHPYPFEKLRALLADAGKPTHDLPPINLSIGEPKHAAPACVGQAIAANLAGLS 60 MN L L PYPFEKLRALL KP D I LSIGEPKH +PA V QA+A NL L+ Sbjct: 1 MNHALTQLQPYPFEKLRALLGSV-KPAADKRAIALSIGEPKHESPAFVAQAMADNLDKLA 59 Query: 61 VYPSTKGEPALRQAISQWLSRRYSIPAP--DPESEVLPVLGSREALFAFAQTVIDPSAGA 118 VYPST G PALRQAI QW RR+ +PA D + +LPV G+REALFAF Q V++ + Sbjct: 60 VYPSTLGLPALRQAIGQWCERRFGVPAGWLDADRHILPVNGTREALFAFTQAVVNRADDG 119 Query: 119 LVVCPNPFYQIYEGAALLAGATPYYVNADPARDFGLRTGRVPDEVWRRTQLVFVCSPGNP 178 LV+ PNPFYQIYEGAALLAGATP+Y+ F VP EVW+R Q++F+CSPGNP Sbjct: 120 LVISPNPFYQIYEGAALLAGATPHYLPCLENNGFNPDFDAVPAEVWKRCQILFLCSPGNP 179 Query: 179 AGNVMSLEEWRTLFELSDRHGFVIAAYECYSEIYLDEDTPPLGSLQAARRLGRDRYTNLV 238 G ++ + + L L+D H FVIAA ECYSE+Y DED PP G L A LGR + V Sbjct: 180 TGALVPMNTLKKLIALADEHDFVIAADECYSELYFDEDAPPPGLLSACAELGRSDFARCV 239 Query: 239 AFSSLSKRSNVPGMRSGFVAGDAALLARFLLYRTYHGSAMSPVVSAASIAAWSMRRMCRK 298 F SLSKRSN+PG+RSGFVAGDA ++ FLLYRTYHG AM ASIAAW R+ Sbjct: 240 VFHSLSKRSNLPGLRSGFVAGDAQIIKPFLLYRTYHGCAMPVQTQLASIAAWQDEAHVRE 299 Query: 299 TA-QYRAKFEAVLPILQNVLDVRAPQASFYLWAGTPGSDTAFARELYGRTGVTVLPGSLL 357 QYRAK++AVL ILQ VLDV+ P SFYLWA PG D F R+L+ VTV+PGS L Sbjct: 300 NRDQYRAKYDAVLDILQPVLDVQRPDGSFYLWAKVPGDDAEFTRDLFEAQHVTVVPGSYL 359 Query: 358 AREAHNANPGQGRIRIALVAPLDQCVQAAERIAHF 392 +RE NPG GR+R+ALVAPL +C++AAERI F Sbjct: 360 SREVDGVNPGAGRVRMALVAPLAECIEAAERIRAF 394 Lambda K H 0.321 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 540 Number of extensions: 28 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 397 Length of database: 398 Length adjustment: 31 Effective length of query: 366 Effective length of database: 367 Effective search space: 134322 Effective search space used: 134322 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 50 (23.9 bits)
Align candidate PP_1588 PP_1588 (N-succinyl-L,L-diaminopimelate aminotransferase alternative)
to HMM TIGR03538 (dapC: succinyldiaminopimelate transaminase (EC 2.6.1.17))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR03538.hmm # target sequence database: /tmp/gapView.25382.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03538 [M=395] Accession: TIGR03538 Description: DapC_gpp: succinyldiaminopimelate transaminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.1e-208 678.9 0.0 1.2e-208 678.7 0.0 1.0 1 lcl|FitnessBrowser__Putida:PP_1588 PP_1588 N-succinyl-L,L-diaminopi Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Putida:PP_1588 PP_1588 N-succinyl-L,L-diaminopimelate aminotransferase alternative # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 678.7 0.0 1.2e-208 1.2e-208 1 395 [] 1 395 [. 1 395 [. 1.00 Alignments for each domain: == domain 1 score: 678.7 bits; conditional E-value: 1.2e-208 TIGR03538 1 mnpnlerlkpyPfeklaellkdvtppadleeialsiGePkhatPafvlealvenleelskyPttkGlpelreaia 75 mn++l++l+pyPfekl++ll +v+p+ad+++ialsiGePkh +Pafv++a+++nl++l+ yP+t Glp+lr+ai lcl|FitnessBrowser__Putida:PP_1588 1 MNHALTQLQPYPFEKLRALLGSVKPAADKRAIALSIGEPKHESPAFVAQAMADNLDKLAVYPSTLGLPALRQAIG 75 9************************************************************************** PP TIGR03538 76 eWlerrfelpag.vdperqvlPvnGtrealfafvqavidraekalvvlPnPfyqiyeGaallagaepyflnctae 149 +W+errf +pag +d++r++lPvnGtrealfaf+qav++ra++ lv++PnPfyqiyeGaallaga+p++l+c ++ lcl|FitnessBrowser__Putida:PP_1588 76 QWCERRFGVPAGwLDADRHILPVNGTREALFAFTQAVVNRADDGLVISPNPFYQIYEGAALLAGATPHYLPCLEN 150 *************************************************************************** PP TIGR03538 150 ngfkpdfdavpeevWkrvqllfvcsPgnPtGavlsleelkklleladkydfiiasdecyselyldeaeaPvGlle 224 ngf+pdfdavp+evWkr+q+lf+csPgnPtGa++++++lkkl++lad++df+ia+decysely+de+++P Gll+ lcl|FitnessBrowser__Putida:PP_1588 151 NGFNPDFDAVPAEVWKRCQILFLCSPGNPTGALVPMNTLKKLIALADEHDFVIAADECYSELYFDEDAPPPGLLS 225 *************************************************************************** PP TIGR03538 225 aaaelGrddfkrllvfhslskrsnvPGlrsGfvaGdaellkeflryrtyhGcampiavqlasiaaWedekhvren 299 a+aelGr+df r++vfhslskrsn+PGlrsGfvaGda+++k fl yrtyhGcamp+++qlasiaaW+de+hvren lcl|FitnessBrowser__Putida:PP_1588 226 ACAELGRSDFARCVVFHSLSKRSNLPGLRSGFVAGDAQIIKPFLLYRTYHGCAMPVQTQLASIAAWQDEAHVREN 300 *************************************************************************** PP TIGR03538 300 ralyrekfaavleilgavldlelPdasfylWlkvpdgddeafaralyeeenvkvlpGrylsreaegvnPGegrvr 374 r++yr+k++avl+il++vld+++Pd+sfylW+kv +gdd++f+r+l+e+++v+v+pG+ylsre++gvnPG+grvr lcl|FitnessBrowser__Putida:PP_1588 301 RDQYRAKYDAVLDILQPVLDVQRPDGSFYLWAKV-PGDDAEFTRDLFEAQHVTVVPGSYLSREVDGVNPGAGRVR 374 **********************************.5*************************************** PP TIGR03538 375 lalvaeleecveaaerikkll 395 +alva+l+ec+eaaeri+++l lcl|FitnessBrowser__Putida:PP_1588 375 MALVAPLAECIEAAERIRAFL 395 ******************985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (395 nodes) Target sequences: 1 (398 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 11.17 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory