Aligments for a candidate for mesD in Pseudomonas putida KT2440

GapMind for Amino acid biosynthesis

Aligments for a candidate for mesD in Pseudomonas putida KT2440

Align 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase; EC 2.1.1.14 (characterized, see rationale)
to candidate PP_2698 PP_2698 5-methyltetrahydropteroyltriglutamate- homocysteine methyltransferase

Query= uniprot:Q6F6Z8
         (344 letters)



>lcl|FitnessBrowser__Putida:PP_2698 PP_2698
           5-methyltetrahydropteroyltriglutamate- homocysteine
           methyltransferase
          Length = 344

 Score =  607 bits (1566), Expect = e-178
 Identities = 295/341 (86%), Positives = 318/341 (93%)

Query: 3   MKRVLPTSTAGSLPKPSWIAEPEKLWSPWKLEGADLAQGKRDALLVSLQEQLHAGIDIVS 62
           M ++LPTSTAGSLPKP+W+A+PE LWSPWKL+   L +GK+DAL ++LQEQ HAGIDIVS
Sbjct: 1   MNKLLPTSTAGSLPKPAWLAQPETLWSPWKLQDEQLVEGKQDALRLALQEQQHAGIDIVS 60

Query: 63  DGEQTRQHFVTTFIEHLDGVDFAKRETVRIRNRYDASVPTVVGAVSRQKPVFVEDAKFLR 122
           DGEQTRQHFVTTFIEHL GVDF +R+TVRIR+RYDASVPTVVGAV+RQKPVFVEDAKFLR
Sbjct: 61  DGEQTRQHFVTTFIEHLSGVDFEQRKTVRIRDRYDASVPTVVGAVARQKPVFVEDAKFLR 120

Query: 123 QQTTQPIKWALPGPMTMIDTLYDAHYKSREKLAWEFAKILNQEALELEAAGVDIIQFDEP 182
           Q TTQPIKWALPGPMTMIDTLYD HYKSREKLAWEFAKILNQEA ELEAAGVDIIQFDEP
Sbjct: 121 QHTTQPIKWALPGPMTMIDTLYDNHYKSREKLAWEFAKILNQEARELEAAGVDIIQFDEP 180

Query: 183 AFNVFFDEVNDWGVATLERALEGLKCETAVHICYGYGIKANTDWKKTLGSEWRQYEEAFP 242
           AFNVFFDEVNDWGVATLERA+EGLKCETAVHICYGYGIKANTDWKKTLGSEWRQYE+AFP
Sbjct: 181 AFNVFFDEVNDWGVATLERAIEGLKCETAVHICYGYGIKANTDWKKTLGSEWRQYEQAFP 240

Query: 243 KLQQSKIDIISLECHNSRVPMDLIELIRGKKVMVGAIDVATNTIETPEEVADTLRKALQF 302
           KLQQS IDIISLECHNS VPMDL+ELIRGKKVMVGAIDVAT+ IETPE+VA TLRKALQF
Sbjct: 241 KLQQSSIDIISLECHNSHVPMDLLELIRGKKVMVGAIDVATHVIETPEQVAATLRKALQF 300

Query: 303 VDADKLYPSTNCGMTPLSRDVARGKLQALSAGAEIIRRELS 343
           VDADKLYP TNCGM PL R VA GKL+ALSAGA I+R+EL+
Sbjct: 301 VDADKLYPCTNCGMAPLPRQVASGKLKALSAGAAIVRQELA 341


Lambda     K      H
   0.318    0.134    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 524
Number of extensions: 7
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 344
Length of database: 344
Length adjustment: 29
Effective length of query: 315
Effective length of database: 315
Effective search space:    99225
Effective search space used:    99225
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis