Alignments for a candidate for argH in Shewanella amazonensis SB2B

GapMind for Amino acid biosynthesis

Alignments for a candidate for argH in Shewanella amazonensis SB2B

Align argininosuccinate lyase (EC 4.3.2.1) (characterized)
to candidate 6936063 Sama_0260 argininosuccinate lyase (RefSeq)

Query= ecocyc::ARGSUCCINLYA-MONOMER
         (457 letters)



>FitnessBrowser__SB2B:6936063
          Length = 456

 Score =  588 bits (1517), Expect = e-173
 Identities = 304/453 (67%), Positives = 350/453 (77%)

Query: 1   MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKALVTVGVLTAEEQAQLEE 60
           MALWGGRF   A   F+ FNDSL  DYRL  QD+ GS+AW+ A+ +VG+L A+E ++L+ 
Sbjct: 1   MALWGGRFQGQASALFQLFNDSLPVDYRLFVQDVEGSIAWADAIASVGILNADECSRLKA 60

Query: 61  ALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKKLHTGRSRNDQVATDLKLW 120
           AL  LLE+V      I+ S AEDIHS+VE KLI+KVG LGKKLHTGRSRNDQVATDLKLW
Sbjct: 61  ALTELLEEVGEDESAIIGSGAEDIHSFVETKLIEKVGDLGKKLHTGRSRNDQVATDLKLW 120

Query: 121 CKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRAQPVTFAHWCLAYVEMLARDE 180
           CK +   L T    +  AL+  A    DAVMPGYTHLQRAQPVTF HWCLAYVEML RD 
Sbjct: 121 CKASGGLLETKLTAVIQALLALASRELDAVMPGYTHLQRAQPVTFGHWCLAYVEMLERDL 180

Query: 181 SRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGFASATRNSLDSVSDRDHVLELLS 240
           SRL DALKRL+  PLG GALAGTAY IDR +LA  LGF   T NSLDSVSDRDHV+EL +
Sbjct: 181 SRLADALKRLNTCPLGSGALAGTAYAIDRHELAAALGFGGPTLNSLDSVSDRDHVVELCA 240

Query: 241 AAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSGSSLMPQKKNPDALELIRGKCGRVQ 300
           AA++ M+HLSR AEDLIFFNTGEA FVEL+D VTSGSSLMPQKKNPDALELIRGK GRV 
Sbjct: 241 AASVSMMHLSRMAEDLIFFNTGEAAFVELADNVTSGSSLMPQKKNPDALELIRGKTGRVY 300

Query: 301 GALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDTWLDCLHMAALVLDGIQVKRPRCQEAA 360
           G L G++ T+K LPLAYNKDMQEDKEGLFD LD+W  CL MAALVL+G++V R   ++AA
Sbjct: 301 GNLVGILTTMKALPLAYNKDMQEDKEGLFDTLDSWGICLDMAALVLEGVKVNRDNTKQAA 360

Query: 361 QQGYANATELADYLVAKGVPFREAHHIVGEAVVEAIRQGKPLEDLPLSELQKFSQVIDED 420
           Q GYANATELADYLVAKG+PFREAHH+VGE V+ AI +G+ LE LPL+ LQ F+ VI +D
Sbjct: 361 QAGYANATELADYLVAKGMPFREAHHVVGEVVLHAISKGQALEALPLASLQSFAAVIGDD 420

Query: 421 VYPILSLQSCLDKRAAKGGVSPQQVAQAIAFAQ 453
           VYP LSL  CL KR   GG + +QV  A+A  Q
Sbjct: 421 VYPHLSLDECLAKRDVLGGTAIKQVTAALAAKQ 453


Lambda     K      H
   0.318    0.133    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 576
Number of extensions: 14
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 457
Length of database: 456
Length adjustment: 33
Effective length of query: 424
Effective length of database: 423
Effective search space:   179352
Effective search space used:   179352
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate 6936063 Sama_0260 (argininosuccinate lyase (RefSeq))
to HMM TIGR00838 (argH: argininosuccinate lyase (EC 4.3.2.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00838.hmm
# target sequence database:        /tmp/gapView.7767.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00838  [M=455]
Accession:   TIGR00838
Description: argH: argininosuccinate lyase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
   4.7e-192  624.9   0.0   5.3e-192  624.7   0.0    1.0  1  lcl|FitnessBrowser__SB2B:6936063  Sama_0260 argininosuccinate lyas


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__SB2B:6936063  Sama_0260 argininosuccinate lyase (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  624.7   0.0  5.3e-192  5.3e-192       1     452 [.       3     454 ..       3     456 .] 0.99

  Alignments for each domain:
  == domain 1  score: 624.7 bits;  conditional E-value: 5.3e-192
                         TIGR00838   1 lwggRlkkeldkavaefnaslsfDkelaeaDiegsiahtkaLakagilteeeakklieaLeelkeevkeg.klelev 76 
                                       lwggR++++++  ++ fn+sl  D++l+ +D+egsia++ a+a +gil+++e ++l++aL+el eev e+ ++ + +
  lcl|FitnessBrowser__SB2B:6936063   3 LWGGRFQGQASALFQLFNDSLPVDYRLFVQDVEGSIAWADAIASVGILNADECSRLKAALTELLEEVGEDeSAIIGS 79 
                                       7*****************************************************************9888588999* PP

                         TIGR00838  77 daeDiHlavErelidkvgedvgkklhtgrsRnDqvatdlrlylrdkvkelaealkdllkalvekAekevetlmpgyt 153
                                        aeDiH+ vE++li+kvg d+gkklhtgrsRnDqvatdl+l+++++   l ++l ++++al+ +A +e +++mpgyt
  lcl|FitnessBrowser__SB2B:6936063  80 GAEDIHSFVETKLIEKVG-DLGKKLHTGRSRNDQVATDLKLWCKASGGLLETKLTAVIQALLALASRELDAVMPGYT 155
                                       ******************.********************************************************** PP

                         TIGR00838 154 HLqrAqPitlaHhllayaemlerDleRlldalkRvnksPlGsgAlagtsfeidrellaelLgFdavvensldavsdR 230
                                       HLqrAqP+t++H++lay+emlerDl+Rl dalkR+n++PlGsgAlagt+++idr+ la+ LgF   + nsld+vsdR
  lcl|FitnessBrowser__SB2B:6936063 156 HLQRAQPVTFGHWCLAYVEMLERDLSRLADALKRLNTCPLGSGALAGTAYAIDRHELAAALGFGGPTLNSLDSVSDR 232
                                       ***************************************************************************** PP

                         TIGR00838 231 DfiiEllsaaallmvhlsrlaEelilfsseEfgfvelsdevssgssimPqKKnpDvaEliRgktgrviGnltgllti 307
                                       D+++El +aa++ m+hlsr+aE+li+f++ E+ fvel+d+v+sgss+mPqKKnpD++EliRgktgrv+Gnl+g+lt+
  lcl|FitnessBrowser__SB2B:6936063 233 DHVVELCAAASVSMMHLSRMAEDLIFFNTGEAAFVELADNVTSGSSLMPQKKNPDALELIRGKTGRVYGNLVGILTT 309
                                       ***************************************************************************** PP

                         TIGR00838 308 lKalPlaYnkDlqEdkealfdalktveellevvtgllkelkvnkerleeaakknfalatdlAdylvrkGvPFReahe 384
                                       +KalPlaYnkD+qEdke+lfd+l+ +  +l++++++l+ +kvn++++++aa++++a+at+lAdylv+kG+PFReah+
  lcl|FitnessBrowser__SB2B:6936063 310 MKALPLAYNKDMQEDKEGLFDTLDSWGICLDMAALVLEGVKVNRDNTKQAAQAGYANATELADYLVAKGMPFREAHH 386
                                       ***************************************************************************** PP

                         TIGR00838 385 ivGevvakaiekGkkleeltleelqklsekleedvlevldleeavekrdakGGtakeevekaieeaka 452
                                       +vGevv +ai kG+ le l+l+ lq +   + +dv+  l+l e+++krd+ GGta ++v+ a+++ ++
  lcl|FitnessBrowser__SB2B:6936063 387 VVGEVVLHAISKGQALEALPLASLQSFAAVIGDDVYPHLSLDECLAKRDVLGGTAIKQVTAALAAKQQ 454
                                       **************************************************************998776 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (455 nodes)
Target sequences:                          1  (456 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02
# Mc/sec: 8.15
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis