Align carbamoyl-phosphate synthase (glutamine-hydrolysing) (EC 6.3.5.5) (characterized)
to candidate 6938384 Sama_2503 carbamoyl phosphate synthase large subunit (RefSeq)
Query= BRENDA::P00968 (1073 letters) >FitnessBrowser__SB2B:6938384 Length = 1076 Score = 1802 bits (4667), Expect = 0.0 Identities = 900/1073 (83%), Positives = 980/1073 (91%), Gaps = 1/1073 (0%) Query: 1 MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEM 60 MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEM Sbjct: 1 MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEM 60 Query: 61 ADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALELERQGVLEEFGVTMIGATA 120 ADATYIEPIHWEVVR II KERPDA+LPTMGGQTALNCALELE +GVL+EF V MIGATA Sbjct: 61 ADATYIEPIHWEVVRNIIAKERPDAILPTMGGQTALNCALELEAKGVLKEFNVEMIGATA 120 Query: 121 DAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAADVGFPCIIRPSFTMGGSGGG 180 DAIDKAEDR RFD AMKKIGLE R+GIAH+MEEA AV VGFPCIIRPSFTMGGSGGG Sbjct: 121 DAIDKAEDRARFDKAMKKIGLECPRAGIAHSMEEAYAVLDQVGFPCIIRPSFTMGGSGGG 180 Query: 181 IAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEMEVVRDKNDNCIIVCSIENFDAM 240 IAYN+EEFEEIC++GL+LSPT ELLIDESLIGWKEYEMEVVRD+NDNCIIVCSIENFDAM Sbjct: 181 IAYNKEEFEEICSQGLELSPTNELLIDESLIGWKEYEMEVVRDRNDNCIIVCSIENFDAM 240 Query: 241 GIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIGVETGGSNVQFAVNPKNGRLIVIEM 300 G+HTGDSITVAPAQTLTDKEYQIMRNASMAVLREIGVETGGSNVQF ++P GR++VIEM Sbjct: 241 GVHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIGVETGGSNVQFGISPTTGRMVVIEM 300 Query: 301 NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELMNDITGGRTPASFEPSIDYVVTKIP 360 NPRVSRSSALASKATGFPIAKVAAKLAVG+TLDELMNDITGGRTPASFEPSIDYVVTK+P Sbjct: 301 NPRVSRSSALASKATGFPIAKVAAKLAVGFTLDELMNDITGGRTPASFEPSIDYVVTKVP 360 Query: 361 RFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQESLQKALRGLEVGATGFDPKVSLDDPEA 420 RFNFEKFAGANDRLTTQMKSVGEVMAIGRT QES+QKALRGLEV GFDP V L D +A Sbjct: 361 RFNFEKFAGANDRLTTQMKSVGEVMAIGRTFQESVQKALRGLEVSRNGFDPIVDLSDADA 420 Query: 421 LTKIRRELKDAGADRIWYIADAFRAGLSVDGVFNLTNIDRWFLVQIEELVRLEEKVAEVG 480 L KIR ELK+ GADRIWYI DAFRAGLSV+ +F LTNIDRWFLVQIEE+++LE KV E+G Sbjct: 421 LQKIRHELKEPGADRIWYIGDAFRAGLSVEDIFALTNIDRWFLVQIEEIIQLEAKVKELG 480 Query: 481 ITGLNADFLRQLKRKGFADARLAKLAGVREAEIRKLRDQYDLHPVYKRVDTCAAEFATDT 540 +TG++ DFLR+LKRKGF+DAR++ L G+ E EIRKLR+++++ PVYKRVDTCAAEFATDT Sbjct: 481 VTGIDEDFLRKLKRKGFSDARMSALLGISEGEIRKLRERFNVFPVYKRVDTCAAEFATDT 540 Query: 541 AYMYSTYEEECEANPSTDREKIMVLGGGPNRIGQGIEFDYCCVHASLALREDGYETIMVN 600 AYMYSTYEEECEANPS D++KIMVLGGGPNRIGQGIEFDYCCVHA+LALREDGYETIMVN Sbjct: 541 AYMYSTYEEECEANPS-DKDKIMVLGGGPNRIGQGIEFDYCCVHAALALREDGYETIMVN 599 Query: 601 CNPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVP 660 CNPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEKPKGVIV YGGQTPLKLARALEAAGVP Sbjct: 600 CNPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEKPKGVIVHYGGQTPLKLARALEAAGVP 659 Query: 661 VIGTSPDAIDRAEDRERFQHAVERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVL 720 +IGTSPDAIDRAEDRERFQ A++RL +KQP N TVT +E AV A+ IGYPLVVRPSYVL Sbjct: 660 IIGTSPDAIDRAEDRERFQQAIKRLNMKQPENDTVTTVEGAVIAAERIGYPLVVRPSYVL 719 Query: 721 GGRAMEIVYDEADLRRYFQTAVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIME 780 GGRAMEIVYDE DLRRYF AVSVSN +PVLLD FLDDA EVD+DA+CDGE V+IG IME Sbjct: 720 GGRAMEIVYDEQDLRRYFNEAVSVSNASPVLLDRFLDDATEVDIDAVCDGETVVIGAIME 779 Query: 781 HIEQAGVHSGDSACSLPAYTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLI 840 HIEQAGVHSGDSACSLP YTLS+ IQD MR+QV KLAFEL V GLMNVQFAVK+NE+Y+I Sbjct: 780 HIEQAGVHSGDSACSLPPYTLSKAIQDQMREQVSKLAFELGVVGLMNVQFAVKDNEIYMI 839 Query: 841 EVNPRAARTVPFVSKATGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKF 900 EVNPRAARTVPFVSKATGVPLAK+AARVMAG+SL +Q TKEVIPP+YSVKEVVLPFNKF Sbjct: 840 EVNPRAARTVPFVSKATGVPLAKIAARVMAGQSLKQQNFTKEVIPPFYSVKEVVLPFNKF 899 Query: 901 PGVDPLLGPEMRSTGEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDL 960 PGVDPLLGPEMRSTGEVMGVG TFAEA+AK++LGS + + GRALLSVR DK +V DL Sbjct: 900 PGVDPLLGPEMRSTGEVMGVGDTFAEAYAKSELGSIKDVPRSGRALLSVRNSDKNKVADL 959 Query: 961 AAKLLKQGFELDATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGR 1020 AAKLL+ G+ELDATHGTA++LGEAGINPRLVNKVHEGRPHI DRIKNGEY YI+NTT GR Sbjct: 960 AAKLLELGYELDATHGTAVILGEAGINPRLVNKVHEGRPHILDRIKNGEYAYIVNTTEGR 1019 Query: 1021 RAIEDSRVIRRSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK 1073 +AIEDSR +RR+AL+YKV+Y TTLN FA MA NAD KV SVQE+H ++K Sbjct: 1020 QAIEDSRQLRRAALRYKVNYTTTLNAAFANCMAHNADDRNKVASVQELHERVK 1072 Lambda K H 0.318 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 3369 Number of extensions: 130 Number of successful extensions: 12 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 1073 Length of database: 1076 Length adjustment: 45 Effective length of query: 1028 Effective length of database: 1031 Effective search space: 1059868 Effective search space used: 1059868 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 58 (26.9 bits)
Align candidate 6938384 Sama_2503 (carbamoyl phosphate synthase large subunit (RefSeq))
to HMM TIGR01369 (carB: carbamoyl-phosphate synthase, large subunit (EC 6.3.5.5))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01369.hmm # target sequence database: /tmp/gapView.19006.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01369 [M=1052] Accession: TIGR01369 Description: CPSaseII_lrg: carbamoyl-phosphate synthase, large subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 0 1584.8 0.0 0 1584.6 0.0 1.0 1 lcl|FitnessBrowser__SB2B:6938384 Sama_2503 carbamoyl phosphate sy Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__SB2B:6938384 Sama_2503 carbamoyl phosphate synthase large subunit (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 1584.6 0.0 0 0 1 1050 [. 2 1051 .. 2 1053 .. 0.98 Alignments for each domain: == domain 1 score: 1584.6 bits; conditional E-value: 0 TIGR01369 1 pkredikkvlviGsGpivigqAaEFDYsGsqalkalkeegievvLvnsniAtvmtdeeladkvYiePltveavek 75 pkr+dik++l++G+GpivigqA+EFDYsG+qa+kal+eeg++v+Lvnsn+At+mtd+e+ad++YieP+++e+v++ lcl|FitnessBrowser__SB2B:6938384 2 PKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEMADATYIEPIHWEVVRN 76 689************************************************************************ PP TIGR01369 76 iiekErpDailltlGGqtaLnlaveleekGvLekygvkllGtkveaikkaedRekFkealkeineevakseives 150 ii kErpDail+t+GGqtaLn+a+ele kGvL++++v+++G++ +ai+kaedR +F++a+k+i++e +++ i++s lcl|FitnessBrowser__SB2B:6938384 77 IIAKERPDAILPTMGGQTALNCALELEAKGVLKEFNVEMIGATADAIDKAEDRARFDKAMKKIGLECPRAGIAHS 151 *************************************************************************** PP TIGR01369 151 veealeaaeeigyPvivRaaftlgGtGsgiaeneeelkelvekalkaspikqvlvekslagwkEiEyEvvRDskd 225 +eea ++ +++g+P+i+R++ft+gG+G+gia+n+ee++e+++++l++sp++++l+++sl gwkE+E+EvvRD++d lcl|FitnessBrowser__SB2B:6938384 152 MEEAYAVLDQVGFPCIIRPSFTMGGSGGGIAYNKEEFEEICSQGLELSPTNELLIDESLIGWKEYEMEVVRDRND 226 *************************************************************************** PP TIGR01369 226 nciivcniEnlDplGvHtGdsivvaPsqtLtdkeyqllRdaslkiirelgvege.cnvqfaldPeskryvviEvn 299 nciivc+iEn+D++GvHtGdsi+vaP+qtLtdkeyq++R+as++++re+gve++ +nvqf+++P++ r+vviE+n lcl|FitnessBrowser__SB2B:6938384 227 NCIIVCSIENFDAMGVHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIGVETGgSNVQFGISPTTGRMVVIEMN 301 ****************************************************988******************** PP TIGR01369 300 pRvsRssALAskAtGyPiAkvaaklavGysLdelkndvtk.etvAsfEPslDYvvvkiPrwdldkfekvdrklgt 373 pRvsRssALAskAtG+PiAkvaaklavG++Ldel+nd+t+ +t+AsfEPs+DYvv+k+Pr++++kf++++++l+t lcl|FitnessBrowser__SB2B:6938384 302 PRVSRSSALASKATGFPIAKVAAKLAVGFTLDELMNDITGgRTPASFEPSIDYVVTKVPRFNFEKFAGANDRLTT 376 ***************************************879********************************* PP TIGR01369 374 qmksvGEvmaigrtfeealqkalrsleekllglk..lkekeaesdeeleealkkpndrRlfaiaealrrgvsvee 446 qmksvGEvmaigrtf+e++qkalr le + g++ ++ + a + ++++++lk+p ++R+++i +a+r+g+sve+ lcl|FitnessBrowser__SB2B:6938384 377 QMKSVGEVMAIGRTFQESVQKALRGLEVSRNGFDpiVDLSDADALQKIRHELKEPGADRIWYIGDAFRAGLSVED 451 ********************************762233445666788899************************* PP TIGR01369 447 vyeltkidrffleklkklvelekeleeeklkelkkellkkakklGfsdeqiaklvkvseaevrklrkelgivpvv 521 ++ lt+idr+fl +++++++le +++e ++ ++++ l+k+k++Gfsd+++++l++ se e+rklr++ ++ pv+ lcl|FitnessBrowser__SB2B:6938384 452 IFALTNIDRWFLVQIEEIIQLEAKVKELGVTGIDEDFLRKLKRKGFSDARMSALLGISEGEIRKLRERFNVFPVY 526 *************************************************************************** PP TIGR01369 522 krvDtvaaEfeaktpYlYstyeeekddvevtekkkvlvlGsGpiRigqgvEFDycavhavlalreagyktiliny 596 krvDt+aaEf ++t+Y+Ystyeee++ ++ ++k+k++vlG+Gp+Rigqg+EFDyc+vha+lalre gy+ti++n+ lcl|FitnessBrowser__SB2B:6938384 527 KRVDTCAAEFATDTAYMYSTYEEECEANP-SDKDKIMVLGGGPNRIGQGIEFDYCCVHAALALREDGYETIMVNC 600 ************************66655.5556***************************************** PP TIGR01369 597 nPEtvstDydiadrLyFeeltvedvldiiekekvegvivqlgGqtalnlakeleeagvkilGtsaesidraEdRe 671 nPEtvstDyd++drLyFe++t+edvl+i++ ek++gviv++gGqt+l+la++le+agv+i+Gts+++idraEdRe lcl|FitnessBrowser__SB2B:6938384 601 NPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEKPKGVIVHYGGQTPLKLARALEAAGVPIIGTSPDAIDRAEDRE 675 *************************************************************************** PP TIGR01369 672 kFsklldelgikqpkgkeatsveeakeiakeigyPvlvRpsyvlgGrameiveneeeleryleeavevskekPvl 746 +F++++++l++kqp++ ++t+ve a+ +a++igyP++vRpsyvlgGrameiv++e++l+ry++eav+vs+ +Pvl lcl|FitnessBrowser__SB2B:6938384 676 RFQQAIKRLNMKQPENDTVTTVEGAVIAAERIGYPLVVRPSYVLGGRAMEIVYDEQDLRRYFNEAVSVSNASPVL 750 *************************************************************************** PP TIGR01369 747 idkyledavEvdvDavadgeevliagileHiEeaGvHsGDstlvlppqklseevkkkikeivkkiakelkvkGll 821 +d++l+da+Evd+Dav+dge+v+i +i+eHiE+aGvHsGDs+++lpp +ls+ ++++++e+v+k+a el v+Gl+ lcl|FitnessBrowser__SB2B:6938384 751 LDRFLDDATEVDIDAVCDGETVVIGAIMEHIEQAGVHSGDSACSLPPYTLSKAIQDQMREQVSKLAFELGVVGLM 825 *************************************************************************** PP TIGR01369 822 niqfvvkdeevyviEvnvRasRtvPfvskalgvplvklavkvllgkkleelekgvkkekksklvavkaavfsfsk 896 n+qf+vkd+e+y+iEvn+Ra+RtvPfvska+gvpl+k+a++v++g++l++++ +ke + +++vk++v++f+k lcl|FitnessBrowser__SB2B:6938384 826 NVQFAVKDNEIYMIEVNPRAARTVPFVSKATGVPLAKIAARVMAGQSLKQQN--FTKEVIPPFYSVKEVVLPFNK 898 **************************************************55..69******************* PP TIGR01369 897 lagvdvvlgpemkstGEvmgigrdleeallkallaskakikkkgsvllsvkdkdkeellelakklaekglkvyat 971 + gvd++lgpem+stGEvmg+g++++ea++k+ l s + +++ g++llsv+++dk+++ +la+kl e+g+++ at lcl|FitnessBrowser__SB2B:6938384 899 FPGVDPLLGPEMRSTGEVMGVGDTFAEAYAKSELGSIKDVPRSGRALLSVRNSDKNKVADLAAKLLELGYELDAT 973 *************************************************************************** PP TIGR01369 972 egtakvleeagikaevvlkvseeaekilellkeeeielvinltskkkkaaekgykirreaveykvplvteletae 1046 +gta +l eagi+ ++v+kv+e +++il+ +k++e+ +++n+t+ +++a e++ ++rr+a++ykv++ t+l++a lcl|FitnessBrowser__SB2B:6938384 974 HGTAVILGEAGINPRLVNKVHEGRPHILDRIKNGEYAYIVNTTE-GRQAIEDSRQLRRAALRYKVNYTTTLNAAF 1047 *****************************************997.88899999*****************99987 PP TIGR01369 1047 alle 1050 a ++ lcl|FitnessBrowser__SB2B:6938384 1048 ANCM 1051 7665 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (1052 nodes) Target sequences: 1 (1076 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.06u 0.02s 00:00:00.08 Elapsed: 00:00:00.08 # Mc/sec: 13.30 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory