Alignments for a candidate for asd in Shewanella amazonensis SB2B

GapMind for Amino acid biosynthesis

Alignments for a candidate for asd in Shewanella amazonensis SB2B

Align Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 (characterized)
to candidate 6938019 Sama_2152 aspartate-semialdehyde dehydrogenase (RefSeq)

Query= SwissProt::P23247
         (337 letters)



>FitnessBrowser__SB2B:6938019
          Length = 338

 Score =  488 bits (1256), Expect = e-143
 Identities = 239/337 (70%), Positives = 276/337 (81%), Gaps = 1/337 (0%)

Query: 1   MSQQFNVAIFGATGAVGETMLEVLQEREFPVDELFLLASERSEGKTYRFNGKTVRVQNVE 60
           MSQ+FNV + GA+GAVG+TM+E+L+ER FPV +LF LAS RS G T  F+GK V + +VE
Sbjct: 1   MSQEFNVVVLGASGAVGQTMIEILEERNFPVAKLFPLASARSAGDTVSFHGKQVEILDVE 60

Query: 61  EFDWSQVHIALFSAGGELSAKWAPIAAEAGVVVIDNTSHFRYDYDIPLVVPEVNPEAIAE 120
            FDWSQ  I  FSAGG++SAKWAPIAAE G VVIDNTSHFRYD DIPLVVPEVNPEAIA+
Sbjct: 61  TFDWSQAQIGFFSAGGDVSAKWAPIAAEHGCVVIDNTSHFRYDLDIPLVVPEVNPEAIAD 120

Query: 121 FRNRNIIANPNCSTIQMLVALKPIYDAVGIERINVTTYQSVSGAGKAGIDELAGQTAKLL 180
           FRNRNIIANPNCSTIQMLVALKPIYDA GI+RINV TYQSVSG GK  I+ELAGQ AKLL
Sbjct: 121 FRNRNIIANPNCSTIQMLVALKPIYDAYGIDRINVCTYQSVSGTGKKAIEELAGQCAKLL 180

Query: 181 NGYPAETNTFSQQIAFNCIPQIDQFMDNGYTKEEMKMVWETQKIFNDPSIMVNPTCVRVP 240
            G P E + + +QIAFN +P ID+F DNGYTKEEMKMVWET+KIF D SI+VNPT VRVP
Sbjct: 181 QGLPIEPSVYPKQIAFNVLPHIDKFQDNGYTKEEMKMVWETRKIFGDDSILVNPTAVRVP 240

Query: 241 VFYGHAEAVHVETRAPIDAEQVMDMLEQTDGIELFR-GADFPTQVRDAGGKDHVLVGRVR 299
           VFYGH+EAVH+ETR P+DA+ V  +L    G+ LF    D+PT V D  G+D V VGRVR
Sbjct: 241 VFYGHSEAVHIETRDPVDADTVKALLRDAPGVVLFEDDDDYPTAVTDGAGQDPVFVGRVR 300

Query: 300 NDISHHSGINLWVVADNVRKGAATNAVQIAELLVRDY 336
           NDISH +GINLWVV+DN+RKGAA N+VQI E+L+RDY
Sbjct: 301 NDISHPNGINLWVVSDNIRKGAALNSVQIGEILIRDY 337


Lambda     K      H
   0.319    0.135    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 410
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 337
Length of database: 338
Length adjustment: 28
Effective length of query: 309
Effective length of database: 310
Effective search space:    95790
Effective search space used:    95790
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate 6938019 Sama_2152 (aspartate-semialdehyde dehydrogenase (RefSeq))
to HMM TIGR01296 (asd: aspartate-semialdehyde dehydrogenase (EC 1.2.1.11))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01296.hmm
# target sequence database:        /tmp/gapView.1124.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01296  [M=339]
Accession:   TIGR01296
Description: asd_B: aspartate-semialdehyde dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
   4.6e-135  436.0   0.0   5.2e-135  435.8   0.0    1.0  1  lcl|FitnessBrowser__SB2B:6938019  Sama_2152 aspartate-semialdehyde


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__SB2B:6938019  Sama_2152 aspartate-semialdehyde dehydrogenase (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  435.8   0.0  5.2e-135  5.2e-135       1     338 [.       6     335 ..       6     336 .. 0.98

  Alignments for each domain:
  == domain 1  score: 435.8 bits;  conditional E-value: 5.2e-135
                         TIGR01296   1 nvaivGatGavGqellkvLeernfpidklvllasersaGkkvkfkgkeleveeaekesfegidialfsaGgsvskef 77 
                                       nv ++Ga+GavGq+++++Leernfp+ kl +las+rsaG  v f+gk++e+ ++e+++ ++ +i +fsaGg vs ++
  lcl|FitnessBrowser__SB2B:6938019   6 NVVVLGASGAVGQTMIEILEERNFPVAKLFPLASARSAGDTVSFHGKQVEILDVETFDWSQAQIGFFSAGGDVSAKW 82 
                                       689************************************************************************** PP

                         TIGR01296  78 apkaakagviviDntsafrldedvPLvvpevnaeelkeakkkgiianPnCstiqlvvvLkplkdeaklkrvvvstYq 154
                                       ap aa++g++viDnts fr d d+PLvvpevn e ++++++++iianPnCstiq++v+Lkp++d++++ r+ v tYq
  lcl|FitnessBrowser__SB2B:6938019  83 APIAAEHGCVVIDNTSHFRYDLDIPLVVPEVNPEAIADFRNRNIIANPNCSTIQMLVALKPIYDAYGIDRINVCTYQ 159
                                       ***************************************************************************** PP

                         TIGR01296 155 avsGaGkkgveeLknqtkavlegkekepeidalkakkfakqiafnaiplidklkedGytkeelkllfetrkilgied 231
                                       +vsG+Gkk++eeL+ q    l+g  +ep       + ++kqiafn++p+idk++++Gytkee+k+++etrki+g++ 
  lcl|FitnessBrowser__SB2B:6938019 160 SVSGTGKKAIEELAGQCAKLLQGLPIEP-------SVYPKQIAFNVLPHIDKFQDNGYTKEEMKMVWETRKIFGDDS 229
                                       *****************99999998886.......89**************************************** PP

                         TIGR01296 232 lkvsatcvrvPvftghsesvsiefekelsveevkelLkeapgvvviddpsenlyptPl.eavgkdevfvgrirkDls 307
                                       + v  t+vrvPvf+ghse+v+ie+ ++++++ vk lL++apgvv+ +d+  + ypt + + +g+d vfvgr+r+D+s
  lcl|FitnessBrowser__SB2B:6938019 230 ILVNPTAVRVPVFYGHSEAVHIETRDPVDADTVKALLRDAPGVVLFEDD--DDYPTAVtDGAGQDPVFVGRVRNDIS 304
                                       **********************************************999..78999873689*************** PP

                         TIGR01296 308 kekglalfvvaDnlrkGaalnavqiaellik 338
                                       + +g++l+vv+Dn+rkGaaln+vqi e li+
  lcl|FitnessBrowser__SB2B:6938019 305 HPNGINLWVVSDNIRKGAALNSVQIGEILIR 335
                                       ***************************9997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (339 nodes)
Target sequences:                          1  (338 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 8.55
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis