Align Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EPSP phospholyase; EC 4.2.3.5 (characterized)
to candidate SMc00007 SMc00007 chorismate synthase
Query= SwissProt::P12008 (361 letters) >FitnessBrowser__Smeli:SMc00007 Length = 365 Score = 409 bits (1052), Expect = e-119 Identities = 214/368 (58%), Positives = 267/368 (72%), Gaps = 16/368 (4%) Query: 1 MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHDLDRRRPGTSRYTTQRR 60 M+ NT G LFRVTT+GESHG ALGC+VDG PPGI T A++Q LD+R+PG SR+ TQRR Sbjct: 1 MSHNTFGHLFRVTTWGESHGPALGCVVDGCPPGIRFTLAEVQAWLDKRKPGQSRFVTQRR 60 Query: 61 EPDQVKILSGVF---EG---VTTGTSIGLLIENTDQRSQDYSAIKDVFRPGHADYTYEQK 114 E D VK+LSGV +G ++TGT I ++IENTDQRS+DYS I +RPGHADYTY+ K Sbjct: 61 EDDLVKVLSGVMLDDDGETMISTGTPISMMIENTDQRSKDYSEIAKRYRPGHADYTYDAK 120 Query: 115 YGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQMGDIPLDIK--DWSQ 172 YG+RDYRGGGRSSARETA RVAAGAIA+K + G+ +RG L Q+G +D DW++ Sbjct: 121 YGIRDYRGGGRSSARETAARVAAGAIARKVVP---GLVVRGALVQIGKHRIDRSNWDWAE 177 Query: 173 VEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGLGEPVFDRLDADIAH 232 V NPFF PDP + +E + ++K G SIGA V VVA GVPAG+G P++ +LD DIA Sbjct: 178 VNNNPFFAPDPAIVPVWEEYLDGIRKAGSSIGAVVEVVAEGVPAGIGAPIYAKLDQDIAA 237 Query: 233 ALMSINAVKGVEIGDGFDVVALRGSQNRDEIT-----KDGFQSNHAGGILGGISSGQQII 287 LMSINAVKGVEIG+GF + G +N DE+ + F SN+AGGILGGI++GQ ++ Sbjct: 238 NLMSINAVKGVEIGNGFAAAEISGEENADEMRIGAQGEPVFLSNNAGGILGGIATGQPVV 297 Query: 288 AHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRQ 347 A A+KPTSSI R+I+ G+EV++ TKGRHDPCVGIRAVPI EAMLA + DH LR Sbjct: 298 ARFAIKPTSSILTERRSIDSDGKEVDVRTKGRHDPCVGIRAVPIGEAMLACAIADHYLRD 357 Query: 348 RAQNADVK 355 R Q +K Sbjct: 358 RGQTGRLK 365 Lambda K H 0.319 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 457 Number of extensions: 17 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 361 Length of database: 365 Length adjustment: 29 Effective length of query: 332 Effective length of database: 336 Effective search space: 111552 Effective search space used: 111552 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
Align candidate SMc00007 SMc00007 (chorismate synthase)
to HMM TIGR00033 (aroC: chorismate synthase (EC 4.2.3.5))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00033.hmm # target sequence database: /tmp/gapView.21774.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00033 [M=351] Accession: TIGR00033 Description: aroC: chorismate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.8e-126 405.9 0.1 7.8e-126 405.7 0.1 1.0 1 lcl|FitnessBrowser__Smeli:SMc00007 SMc00007 chorismate synthase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Smeli:SMc00007 SMc00007 chorismate synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 405.7 0.1 7.8e-126 7.8e-126 1 350 [. 10 360 .. 10 361 .. 0.97 Alignments for each domain: == domain 1 score: 405.7 bits; conditional E-value: 7.8e-126 TIGR00033 1 lrlttfGeSHgkalgaiidGlPaglelteediqkelkrRrpgqsrltrmrkEeDeveilsGvf...eGkT...tG 69 +r+tt+GeSHg+alg+++dG+P+g++ t +++q l++R+pgqsr+ ++r+E+D v++lsGv+ G+T tG lcl|FitnessBrowser__Smeli:SMc00007 10 FRVTTWGESHGPALGCVVDGCPPGIRFTLAEVQAWLDKRKPGQSRFVTQRREDDLVKVLSGVMlddDGETmisTG 84 89************************************************************755579985557* PP TIGR00033 70 aPiallikNkdvrskdyedikelpRPgHadytylkKYgikdregggrsSaReTaarvaaGavakklLketagiei 144 +Pi+++i+N+d+rskdy++i++++RPgHadyty KYgi+d++gggrsSaReTaarvaaGa+a+k + + + lcl|FitnessBrowser__Smeli:SMc00007 85 TPISMMIENTDQRSKDYSEIAKRYRPGHADYTYDAKYGIRDYRGGGRSSARETAARVAAGAIARKVVPG---LVV 156 *****************************************************************9987...679 PP TIGR00033 145 vayvvklgeveleeesakeiskerldkspvrcpdaeaekemeeeidkakkdgdsvGgvvevvvsnvpvglGeplf 219 + +v++g+ ++++++ + +++++p+++pd+ ee++d ++k+g s+G+vvevv+++vp+g+G p++ lcl|FitnessBrowser__Smeli:SMc00007 157 RGALVQIGKHRIDRSNWDW---AEVNNNPFFAPDPAIVPVWEEYLDGIRKAGSSIGAVVEVVAEGVPAGIGAPIY 228 9************998884...68999************************************************ PP TIGR00033 220 dkldaelasallsinAvKgveiGdGFeaasvrGseanDelvle.ddkirrktnnsGGieGGitnGedirvriavK 293 kld +a+ l+sinAvKgveiG+GF+aa++ G e De+ + +++ + +nn GGi+GGi++G++++ r a+K lcl|FitnessBrowser__Smeli:SMc00007 229 AKLDQDIAANLMSINAVKGVEIGNGFAAAEISGEENADEMRIGaQGEPVFLSNNAGGILGGIATGQPVVARFAIK 303 *****************************************998999**************************** PP TIGR00033 294 piptikkplktvdletkekakatkgRhDpcvvpravpvvEamvalvladallekras 350 p+++i ++++++d ++ke +tkgRhDpcv +ravp+ Eam+a +ad++l++r++ lcl|FitnessBrowser__Smeli:SMc00007 304 PTSSILTERRSIDSDGKEVDVRTKGRHDPCVGIRAVPIGEAMLACAIADHYLRDRGQ 360 ****************************************************98875 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (351 nodes) Target sequences: 1 (365 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.33 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory