Aligments for a candidate for lysN in Sinorhizobium meliloti 1021

GapMind for Amino acid biosynthesis

Aligments for a candidate for lysN in Sinorhizobium meliloti 1021

Align 2-aminoadipate transaminase (EC 2.6.1.39) (characterized)
to candidate SMc04323 SMc04323 aminotransferase

Query= BRENDA::Q72LL6
         (397 letters)



>lcl|FitnessBrowser__Smeli:SMc04323 SMc04323 aminotransferase
          Length = 408

 Score =  299 bits (766), Expect = 9e-86
 Identities = 174/404 (43%), Positives = 241/404 (59%), Gaps = 12/404 (2%)

Query: 4   LSWSEAFGKGAGRIQASTIRELLKLTQRPGILSFAGGLPAPELFPKEEAAEAAARILREK 63
           L W   F   + R++AS IRELLKL  RP I+SFAGG+P PELFP +   EA A I    
Sbjct: 2   LDWESIFATRSSRMKASEIRELLKLLDRPDIISFAGGIPDPELFPNDAFREAYAEIFGGP 61

Query: 64  GE-VALQYSPTEGYAPLRAFVAEW-----IGVRPEEVLITTGSQQALDLVGKVFLDEGSP 117
               ALQYS +EGY PLR ++A       I    + + IT+GSQQ LD +GK+FL     
Sbjct: 62  SVGAALQYSISEGYRPLREWLAGQMAALGIPASVDNIFITSGSQQGLDYLGKLFLSPKDT 121

Query: 118 VLLEAPSYMGAIQAFRLQGPRFLTV-PAGEEGPDLDALEEVLKRERPRFLYLIPSFQNPT 176
            L+  P+Y+GA+QAF    P +  + PAG   P   A        R +F YL   F NPT
Sbjct: 122 ALVTWPTYLGALQAFNAYEPSYDQLNPAGNRTPAAYAQAAAEAGGRVKFAYLSADFANPT 181

Query: 177 GGLTPLPARKRLLQMVMERGLVVVEDDAYREL-YFGEARLPSL-FELAREAGYPGV--IY 232
           G       R+R+L++  E  + ++ED AY+ L Y GEA  P L  E+AR+        IY
Sbjct: 182 GETVGRAGRERVLELAEELDIAIIEDAAYQSLRYDGEAIPPILALEIARKGDINSTRTIY 241

Query: 233 LGSFSKVLSPGLRVAFAVAHPEALQKLVQAKQGADLHTPMLNQMLVHELLKEGFSERLER 292
            GSFSK L+PGLRV +  A    ++KLV  KQ ADLH+  +NQM +  + + GF E++ +
Sbjct: 242 CGSFSKTLAPGLRVGWICAAEPVIRKLVLMKQAADLHSSTINQMAIATVAERGFEEQVAK 301

Query: 293 VRRVYREKAQAMLHALDREVPKEVRYTRPKGGMFVWMELPKGLSAEGLFRRALE-ENVAF 351
           + + YR++  AML AL++ +P  V +T+P+GGMF+W+ LPKG     L  ++++   VAF
Sbjct: 302 IHKAYRQRRDAMLSALEKYMPAGVTWTKPEGGMFIWVTLPKGSDGAELLAKSIQTAKVAF 361

Query: 352 VPGGPFFANGGGENTLRLSYATLDREGIAEGVRRLGRALKGLLA 395
           VPG  FFA+G GENTLRLS++  +   I EG+RRLG  ++G +A
Sbjct: 362 VPGRAFFADGSGENTLRLSFSCANDRMIDEGIRRLGDLVRGEVA 405


Lambda     K      H
   0.320    0.139    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 474
Number of extensions: 28
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 397
Length of database: 408
Length adjustment: 31
Effective length of query: 366
Effective length of database: 377
Effective search space:   137982
Effective search space used:   137982
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis