Align acetylornithine deacetylase (EC 3.5.1.16) (characterized)
to candidate SMa1836 SMa1836 acetylornithine deacetylase
Query= BRENDA::Q92Y75 (374 letters) >FitnessBrowser__Smeli:SMa1836 Length = 374 Score = 745 bits (1923), Expect = 0.0 Identities = 374/374 (100%), Positives = 374/374 (100%) Query: 1 MQAAEILGKLVGFRSVVGLPNNDVVSWIRGYLESHGIAVDVLPGPEGDRSNIFATIGPKE 60 MQAAEILGKLVGFRSVVGLPNNDVVSWIRGYLESHGIAVDVLPGPEGDRSNIFATIGPKE Sbjct: 1 MQAAEILGKLVGFRSVVGLPNNDVVSWIRGYLESHGIAVDVLPGPEGDRSNIFATIGPKE 60 Query: 61 ARGYIISGHMDVVPAAETGWTSDPFRLRVEADRLYGRGTTDMKGFLAAVLAAVPKLAAMP 120 ARGYIISGHMDVVPAAETGWTSDPFRLRVEADRLYGRGTTDMKGFLAAVLAAVPKLAAMP Sbjct: 61 ARGYIISGHMDVVPAAETGWTSDPFRLRVEADRLYGRGTTDMKGFLAAVLAAVPKLAAMP 120 Query: 121 LRRPLHLALSYDEEAGCRGVPHMIARLPELCRQPLGAIIGEPTGMRAIRAHKGKAAARLT 180 LRRPLHLALSYDEEAGCRGVPHMIARLPELCRQPLGAIIGEPTGMRAIRAHKGKAAARLT Sbjct: 121 LRRPLHLALSYDEEAGCRGVPHMIARLPELCRQPLGAIIGEPTGMRAIRAHKGKAAARLT 180 Query: 181 VRGRSGHSSRPDQGLNAIHGVAGVLTQAVAEADRLVGGPFEHVFEPPYSSLQIGTVKGGQ 240 VRGRSGHSSRPDQGLNAIHGVAGVLTQAVAEADRLVGGPFEHVFEPPYSSLQIGTVKGGQ Sbjct: 181 VRGRSGHSSRPDQGLNAIHGVAGVLTQAVAEADRLVGGPFEHVFEPPYSSLQIGTVKGGQ 240 Query: 241 AVNIIPDSCEVEFEARAISGVDPAELLAPVRKTAEALTTLGFEVEWQELSAYPALSLEPD 300 AVNIIPDSCEVEFEARAISGVDPAELLAPVRKTAEALTTLGFEVEWQELSAYPALSLEPD Sbjct: 241 AVNIIPDSCEVEFEARAISGVDPAELLAPVRKTAEALTTLGFEVEWQELSAYPALSLEPD 300 Query: 301 APLAALLEELTGREALPAVSYGTEAGLFQRAGIDAIICGPGDIGRAHKPDEYILIDELMA 360 APLAALLEELTGREALPAVSYGTEAGLFQRAGIDAIICGPGDIGRAHKPDEYILIDELMA Sbjct: 301 APLAALLEELTGREALPAVSYGTEAGLFQRAGIDAIICGPGDIGRAHKPDEYILIDELMA 360 Query: 361 CRAMVEALGARCTA 374 CRAMVEALGARCTA Sbjct: 361 CRAMVEALGARCTA 374 Lambda K H 0.319 0.137 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 633 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 374 Length of database: 374 Length adjustment: 30 Effective length of query: 344 Effective length of database: 344 Effective search space: 118336 Effective search space used: 118336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate SMa1836 SMa1836 (acetylornithine deacetylase)
to HMM TIGR01892 (argE: acetylornithine deacetylase (ArgE) (EC 3.5.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01892.hmm # target sequence database: /tmp/gapView.29902.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01892 [M=365] Accession: TIGR01892 Description: AcOrn-deacetyl: acetylornithine deacetylase (ArgE) Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9.7e-174 563.5 0.0 1.1e-173 563.4 0.0 1.0 1 lcl|FitnessBrowser__Smeli:SMa1836 SMa1836 acetylornithine deacetyl Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Smeli:SMa1836 SMa1836 acetylornithine deacetylase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 563.4 0.0 1.1e-173 1.1e-173 1 364 [. 5 368 .. 5 369 .. 0.99 Alignments for each domain: == domain 1 score: 563.4 bits; conditional E-value: 1.1e-173 TIGR01892 1 eilakLvafdsvsarsnvdlieyvedyleelgvavevlpvadgaeksnllaviGpkegagglvlsGhtDvvPvdea 76 eil+kLv+f+sv++++n+d+++++++yle++g+av+vlp ++g ++sn++a+iGpke a+g+++sGh+DvvP++e+ lcl|FitnessBrowser__Smeli:SMa1836 5 EILGKLVGFRSVVGLPNNDVVSWIRGYLESHGIAVDVLPGPEG-DRSNIFATIGPKE-ARGYIISGHMDVVPAAET 78 79*****************************************.*************.****************** PP TIGR01892 77 aWtsDpfrLtekdgrLYgrGtaDmkGFlalvLaavpdlaaakLkkPlhlvlsaDeevglaGakklieala...rrp 149 +WtsDpfrL+++++rLYgrGt+DmkGFla+vLaavp+laa++L++Plhl+ls+Dee+g++G++++i++l+ r+p lcl|FitnessBrowser__Smeli:SMa1836 79 GWTSDPFRLRVEADRLYGRGTTDMKGFLAAVLAAVPKLAAMPLRRPLHLALSYDEEAGCRGVPHMIARLPelcRQP 154 *********************************************************************9999*** PP TIGR01892 150 alaivGePtslvavRahkGkasakvtvrGreghssrpdrGvsaielaakllarlvaladklkredleeaFtppyat 225 ai+GePt+++a+RahkGka+a++tvrGr+ghssrpd+G++ai+ +a++l+++va+ad+l+++++e++F+ppy++ lcl|FitnessBrowser__Smeli:SMa1836 155 LGAIIGEPTGMRAIRAHKGKAAARLTVRGRSGHSSRPDQGLNAIHGVAGVLTQAVAEADRLVGGPFEHVFEPPYSS 230 **************************************************************************** PP TIGR01892 226 lniGtvkGGkavniiaaaCelvlelRpipGmdpeellallekiaeevkekapgfevkveelsatpaleleedaelv 301 l+iGtvkGG+avnii+++Ce+++e+R+i+G+dp+ella+++k+ae++ +++gfev+++elsa+pal+le+da+l+ lcl|FitnessBrowser__Smeli:SMa1836 231 LQIGTVKGGQAVNIIPDSCEVEFEARAISGVDPAELLAPVRKTAEAL--TTLGFEVEWQELSAYPALSLEPDAPLA 304 *********************************************76..9************************** PP TIGR01892 302 alleklaGa.aaevvsygteagllqelGieavvlGPGdidqahqpdeYveieelkrcrallerl 364 alle+l+G+ a+++vsygteagl+q++Gi+a+++GPGdi++ah+pdeY+ i+el++cra++e+l lcl|FitnessBrowser__Smeli:SMa1836 305 ALLEELTGReALPAVSYGTEAGLFQRAGIDAIICGPGDIGRAHKPDEYILIDELMACRAMVEAL 368 *********99**************************************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (365 nodes) Target sequences: 1 (374 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 9.15 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory