GapMind for Amino acid biosynthesis

 

chorismate biosynthesis in Synechococcus elongatus PCC 7942

Best path

aroG, aroB, aroD, aroE, aroL, aroA, aroC

Also see fitness data for the top candidates

Rules

Overview: Chorismate is the starting point for the biosynthesis of the aromatic amino acids phenylalanine, tryptophan, and tyrosine. Chorismate biosynthesis in GapMind is based on MetaCyc pathways chorismate biosynthesis I (link), from D-erythrose-4-phosphate and phosphoenolpyruvate, or II (link), from D-glyceraldeyde-3-phosphate and L-asparatate. Both pathways are identical after they reach 3-dehydroquinate.

13 steps (11 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
aroG 3-deoxy-7-phosphoheptulonate synthase Synpcc7942_2501
aroB 3-dehydroquinate synthase Synpcc7942_0525
aroD 3-dehydroquinate dehydratase Synpcc7942_0915
aroE shikimate dehydrogenase Synpcc7942_2467
aroL shikimate kinase Synpcc7942_0894
aroA 3-phosphoshikimate 1-carboxyvinyltransferase Synpcc7942_0232
aroC chorismate synthase Synpcc7942_0212
Alternative steps:
aroA' 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase
aroB' dehydroquinate synthase II
asd aspartate semi-aldehyde dehydrogenase Synpcc7942_1848
asp-kinase aspartate kinase Synpcc7942_1001
fbp 6-deoxy-5-ketofructose 1-phosphate synthase Synpcc7942_1443
tpiA D-glyceraldehyde-3-phosphate phospholyase Synpcc7942_1261 Synpcc7942_1116

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory