GapMind for Amino acid biosynthesis


L-histidine biosynthesis in Synechococcus elongatus PCC 7942

Best path

prs, hisG, hisI, hisE, hisA, hisF, hisH, hisB, hisC?, hisN?, hisD

Also see fitness data for the top candidates


Overview: Histidine biosynthesis in GapMind is based on the MetaCyc pathway (link).

11 steps (11 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate Class of gap
prs ribose-phosphate diphosphokinase Synpcc7942_2113  
hisG ATP phosphoribosyltransferase Synpcc7942_0783  
hisI phosphoribosyl-ATP pyrophosphatase Synpcc7942_1995 Synpcc7942_1519  
hisE phosphoribosyl-AMP cyclohydrolase Synpcc7942_1995 Synpcc7942_1519  
hisA isomerase HisA Synpcc7942_1829 Synpcc7942_2087  
hisF IGP synthase, cyclase subunit Synpcc7942_2087 Synpcc7942_1829  
hisH IGP synthase, amidotransferase subunit Synpcc7942_1481  
hisB IGP dehydratase Synpcc7942_0125  
hisC? histidinol-phosphate aminotransferase Synpcc7942_1030 Synpcc7942_1109 diverged
hisN? histidinol-phosphate phosphatase Synpcc7942_1763 Synpcc7942_0125 novel
hisD histidinol dehydrogenase Synpcc7942_1519  

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.



Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory