GapMind for Amino acid biosynthesis

 

Aligments for a candidate for hisE in Synechococcus elongatus PCC 7942

Align putative bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase (EC 3.5.4.19; EC 3.6.1.31) (characterized)
to candidate Synpcc7942_1995 Synpcc7942_1995 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Query= ecocyc::HISTCYCLOPRATPPHOS
         (203 letters)



>lcl|FitnessBrowser__SynE:Synpcc7942_1995 Synpcc7942_1995
           bifunctional phosphoribosyl-AMP
           cyclohydrolase/phosphoribosyl-ATP pyrophosphatase
           protein
          Length = 211

 Score =  147 bits (372), Expect = 1e-40
 Identities = 80/193 (41%), Positives = 115/193 (59%), Gaps = 6/193 (3%)

Query: 17  GLMPVIVQHAVSGEVLMLGYMNPEALDKTLESGKVTFFSRTKQRLWTKGETSGNFLNVVS 76
           GL+P I Q  + G VLML +MN EAL+KTLE+G+V ++SR++Q LW KG TSG+F  + +
Sbjct: 19  GLLPAIAQDYLDGTVLMLAWMNREALEKTLETGEVWYWSRSRQELWHKGATSGHFQKLQA 78

Query: 77  IAPDCDNDTLLVLANPIGP-TCHKGTSSCFGD-----TAHQWLFLYQLEQLLAERKSADP 130
           I  DCD+D LL+    IG   CH G  SCF       T      L ++ +++ +R+    
Sbjct: 79  IRYDCDSDALLLTIEQIGDIACHTGERSCFHRDVDVVTPPPADILTEVYRVVCDRRDRPS 138

Query: 131 ETSYTAKLYASGTKRIAQKVGEEGVETALAATVHDRFELTNEASDLMYHLLVLLQDQGLD 190
           E SYT+KL+A G  +I +K+GEE  E  +A    D   L  EA+DL +H LV L    +D
Sbjct: 139 EASYTSKLFAGGDNKILKKIGEEAAEVVMACKDDDADGLAGEAADLFFHTLVALAQHNVD 198

Query: 191 LTTVIENLRKRHQ 203
           L +V   L++R +
Sbjct: 199 LRSVYRKLQERRR 211


Lambda     K      H
   0.317    0.132    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 118
Number of extensions: 4
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 203
Length of database: 211
Length adjustment: 21
Effective length of query: 182
Effective length of database: 190
Effective search space:    34580
Effective search space used:    34580
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 45 (21.9 bits)

Align candidate Synpcc7942_1995 Synpcc7942_1995 (bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein)
to HMM PF01502 (PRA-CH)

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/PF01502.18.hmm
# target sequence database:        /tmp/gapView.2590.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       PRA-CH  [M=74]
Accession:   PF01502.18
Description: Phosphoribosyl-AMP cyclohydrolase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    2.5e-40  122.5   0.3    4.5e-40  121.7   0.3    1.4  1  lcl|FitnessBrowser__SynE:Synpcc7942_1995  Synpcc7942_1995 bifunctional pho


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__SynE:Synpcc7942_1995  Synpcc7942_1995 bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribos
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  121.7   0.3   4.5e-40   4.5e-40       1      74 []      35     109 ..      35     109 .. 0.98

  Alignments for each domain:
  == domain 1  score: 121.7 bits;  conditional E-value: 4.5e-40
                                               EEEEE-HHHHHHHHHHSB-EEEETTTTEEEETTTTTS--EEEEEEEE-TTSSEEEEEEEESS.-SSTTS CS
                                    PRA-CH   1 mlaymnkealektletgkavyySrsrkklWkKGetSgnvqkvkeirlDcDqDalllkveqkg.aaCHtg 68 
                                               mla+mn+ealektletg+++y+Srsr++lW+KG+tSg++qk+++ir+DcD+Dalll++eq g  aCHtg
  lcl|FitnessBrowser__SynE:Synpcc7942_1995  35 MLAWMNREALEKTLETGEVWYWSRSRQELWHKGATSGHFQKLQAIRYDCDSDALLLTIEQIGdIACHTG 103
                                               9************************************************************956***** PP

                                               BSSS-- CS
                                    PRA-CH  69 kesCFy 74 
                                               ++sCF+
  lcl|FitnessBrowser__SynE:Synpcc7942_1995 104 ERSCFH 109
                                               *****6 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (74 nodes)
Target sequences:                          1  (211 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 6.90
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory