Align Putative (R)-citramalate synthase CimA; EC 2.3.3.21 (uncharacterized)
to candidate Synpcc7942_1410 Synpcc7942_1410 2-isopropylmalate synthase
Query= curated2:O26819 (496 letters) >FitnessBrowser__SynE:Synpcc7942_1410 Length = 540 Score = 356 bits (913), Expect = e-102 Identities = 227/524 (43%), Positives = 302/524 (57%), Gaps = 32/524 (6%) Query: 2 QVRVLDTTLRDGEQTPGVSLTPEEKLRIALKIDALGADIIEAGSAITSEGEREGIRKITS 61 ++ + DTTLRDGEQ+PG SL EEKL IA ++ L DIIEAG A S G+ E +++I + Sbjct: 9 RILIFDTTLRDGEQSPGASLNLEEKLAIARQLARLNVDIIEAGFAFASPGDFEAVQRIAA 68 Query: 62 EGLRAE---ICSFARAVREDIDAAISCDVDS----VHLVVPTSDLHLEHKLRKTREEVLE 114 E + ICS ARA R+DI AA + +H + TSD+HLE+KL+KTR EVL Sbjct: 69 EVGTPDGPTICSLARATRQDIKAAAEALAPAAKGRIHTFIATSDIHLEYKLKKTRAEVLA 128 Query: 115 QAVDCTEYAVDHGILVELSAEDSTRSDMDFLRTIFREGIEAGAERICACDTVGILTPERS 174 + YA VE S ED+ RSD +FL I AGA+ I DTVG TP Sbjct: 129 VIPEMVGYAASLVDDVEFSPEDAGRSDPEFLYECLEAAIAAGAKTINIPDTVGYTTPSEF 188 Query: 175 YEFYRGLSEL-----GAPLSVHCHNDFGLAVANSLAGLRAGASEVHATINGIGERAGNAA 229 G+ + A +SVH HND GLAVAN L ++ GA ++ TINGIGERAGNAA Sbjct: 189 GALIGGIKQNVCNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGERAGNAA 248 Query: 230 LEEVVVALK-----------SLYDVD---TSINIEMLYETSRMVARMTGVYLQPNKAIVG 275 LEE+V+AL D + T +N +Y+TSR+V+ +TG+ +QPNKAIVG Sbjct: 249 LEELVMALHVRRQYFNPFLGRAADSEAPLTQVNTREIYKTSRLVSNLTGMLVQPNKAIVG 308 Query: 276 ENAFAHESGIHADGVLKKAETYEPITPEMVGHGRG-FVMGKHIGTHALRKRLDELGMKVA 334 NAFAHESGIH DGVLK TYE + E +G +GK G +A R RL ELG + Sbjct: 309 ANAFAHESGIHQDGVLKNKLTYEIVDAETIGLSTNRITLGKLSGRNAFRTRLQELGYDLG 368 Query: 335 DDKLMEIFRRVKTLGDMGKCVTDVDLQAIAEDVLGVMEDKVVDLQEVTIVSGNRVTPTAS 394 +D L F R K L D + VTD DL+AI D ++ L+ V + +G+ PTA+ Sbjct: 369 EDDLNRAFLRFKELADKKREVTDRDLEAIVNDET-QQAPELFKLELVQVSAGDHARPTAT 427 Query: 395 VKLRV-DDREVLEAGTGVGPVDAAIVAIKKSLEDFADITLEEYHVDAITGGTDALIDVVI 453 V LR + E+ +A G GPVDA AI + + L E+ V ++T G DA+ +V I Sbjct: 428 VTLRTPEGEELTDAAIGTGPVDAIYRAINRVVN--IPNELIEFSVKSVTAGIDAIGEVTI 485 Query: 454 KLRHGDRIISARSTQPDIIMASVEAFLSGVNRLL-ANEKSEGTH 496 +LRH DRI S S DI++AS +A++ +NRL A +K + H Sbjct: 486 RLRHEDRIFSGHSANTDILVASAQAYIHALNRLAEALQKDKPLH 529 Lambda K H 0.317 0.135 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 702 Number of extensions: 42 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 496 Length of database: 540 Length adjustment: 35 Effective length of query: 461 Effective length of database: 505 Effective search space: 232805 Effective search space used: 232805 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory