Align Carbamoyl-phosphate synthase small chain; Carbamoyl-phosphate synthetase glutamine chain; EC 6.3.5.5 (characterized)
to candidate GFF4700 PS417_24045 carbamoyl phosphate synthase small subunit
Query= SwissProt::P0A6F1 (382 letters) >FitnessBrowser__WCS417:GFF4700 Length = 378 Score = 531 bits (1368), Expect = e-155 Identities = 260/378 (68%), Positives = 299/378 (79%) Query: 1 MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHI 60 M K A+L L DG+ F G AIGA G VGEVVFNT+MTGYQEILTDPSY++QIVTLTYPHI Sbjct: 1 MTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHI 60 Query: 61 GNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYLKRHNIVAIADIDTRKLTR 120 GN GT D ES +V + GLVIRDLPL+ASN+RNT LS YLK +N+VAIA IDTR+LTR Sbjct: 61 GNTGTTPEDVESDRVWSAGLVIRDLPLVASNWRNTMSLSDYLKANNVVAIAGIDTRRLTR 120 Query: 121 LLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAKEVTTAEAYSWTQGSWTLTGG 180 +LREKG+QNGCI+ GDN A+ A+ FPGL GMDLAK V+ E Y W W L Sbjct: 121 ILREKGSQNGCIMVGDNISEEAAIAAAQGFPGLKGMDLAKVVSVKEKYEWRSTVWDLKTD 180 Query: 181 LPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTIVPAQTSAEDVLKMNPDGIFLSN 240 +LP+HVVAYD+G K NILRMLV+RGCR+T+VPAQT A DVL + PDG+FLSN Sbjct: 181 SHATIDAADLPYHVVAYDYGVKYNILRMLVERGCRVTVVPAQTPASDVLALQPDGVFLSN 240 Query: 241 GPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDV 300 GPGDP PCDYAI AI+ LET+IPVFGICLGHQLLALASGAKT+KM GHHG NHPV+D+ Sbjct: 241 GPGDPEPCDYAIQAIKDVLETEIPVFGICLGHQLLALASGAKTLKMGHGHHGANHPVQDL 300 Query: 301 EKNVVMITAQNHGFAVDEATLPANLRVTHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGP 360 + VVMIT+QNHGFAVDEATLP+N+R HKSLFDG+LQGI RTDK AFSFQGHPEASPGP Sbjct: 301 DTGVVMITSQNHGFAVDEATLPSNVRAIHKSLFDGSLQGIERTDKSAFSFQGHPEASPGP 360 Query: 361 HDAAPLFDHFIELIEQYR 378 +D APLFD FI + + R Sbjct: 361 NDVAPLFDRFINEMAKRR 378 Lambda K H 0.318 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 514 Number of extensions: 12 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 382 Length of database: 378 Length adjustment: 30 Effective length of query: 352 Effective length of database: 348 Effective search space: 122496 Effective search space used: 122496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate GFF4700 PS417_24045 (carbamoyl phosphate synthase small subunit)
to HMM TIGR01368 (carA: carbamoyl-phosphate synthase, small subunit (EC 6.3.5.5))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01368.hmm # target sequence database: /tmp/gapView.10912.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01368 [M=361] Accession: TIGR01368 Description: CPSaseIIsmall: carbamoyl-phosphate synthase, small subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.8e-147 477.0 0.0 2e-147 476.8 0.0 1.0 1 lcl|FitnessBrowser__WCS417:GFF4700 PS417_24045 carbamoyl phosphate Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF4700 PS417_24045 carbamoyl phosphate synthase small subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 476.8 0.0 2e-147 2e-147 1 360 [. 5 375 .. 5 376 .. 0.95 Alignments for each domain: == domain 1 score: 476.8 bits; conditional E-value: 2e-147 TIGR01368 1 atlvledGtvfegksfgaekevvGevvFnTsmtGYqEiltDpsYkgqivvltyplignygvneedaeskkikvkg 75 a+l+l+dG++f+g+++ga++++vGevvFnT+mtGYqEiltDpsY++qiv+ltyp+ign+g+++ed+es++++ +g lcl|FitnessBrowser__WCS417:GFF4700 5 AILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHIGNTGTTPEDVESDRVWSAG 79 689************************************************************************ PP TIGR01368 76 lvvkelskevsnyrakesLeeflkeegivaiegvDTRalvkklRekgsmkavistekse.keelvekakespkvk 149 lv+++l +sn+r++ sL+++lk +++vai+g+DTR l++ lRekgs++++i+ ++ +e ++++a+ p +k lcl|FitnessBrowser__WCS417:GFF4700 80 LVIRDLPLVASNWRNTMSLSDYLKANNVVAIAGIDTRRLTRILREKGSQNGCIMVGDNIsEEAAIAAAQGFPGLK 154 *****************************************************99875426778888999***** PP TIGR01368 150 evnlvkevstkeayeleq.........k..akkegkklrvvvidlGvKenilreLvkrgvevtvvpadtsaeeik 213 +++l+k vs+ke+ye+++ + + + + ++vv++d+GvK nilr+Lv+rg++vtvvpa+t+a+++ lcl|FitnessBrowser__WCS417:GFF4700 155 GMDLAKVVSVKEKYEWRStvwdlktdsHatIDAADLPYHVVAYDYGVKYNILRMLVERGCRVTVVPAQTPASDVL 229 *****************977777655414344455559************************************* PP TIGR01368 214 klnpdgillsnGPGdPaaveeaietvkklleakiPifGIclGhqllalalgaktyklkfGhrGaNhpvkdlktgr 288 +l+pdg++lsnGPGdP+ +++ai+ +k +le++iP+fGIclGhqllala+gakt+k+ Gh+GaNhpv+dl+tg lcl|FitnessBrowser__WCS417:GFF4700 230 ALQPDGVFLSNGPGDPEPCDYAIQAIKDVLETEIPVFGICLGHQLLALASGAKTLKMGHGHHGANHPVQDLDTGV 304 *************************************************************************** PP TIGR01368 289 veitsqNHgyavdeeslkeeelevthvnlnDgtveglehkelpvfsvQyHPeaspGphdteylFdefvelik 360 v+itsqNHg+avde++l+++ +++ h++l+Dg+++g+e++++ +fs Q HPeaspGp+d + lFd+f+++++ lcl|FitnessBrowser__WCS417:GFF4700 305 VMITSQNHGFAVDEATLPSN-VRAIHKSLFDGSLQGIERTDKSAFSFQGHPEASPGPNDVAPLFDRFINEMA 375 ****************8866.************************************************996 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (361 nodes) Target sequences: 1 (378 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 6.87 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory