Align 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (characterized)
to candidate GFF1599 PS417_08135 3-phosphoshikimate 1-carboxyvinyltransferase
Query= BRENDA::Q83E11 (438 letters) >FitnessBrowser__WCS417:GFF1599 Length = 736 Score = 446 bits (1148), Expect = e-130 Identities = 237/424 (55%), Positives = 299/424 (70%), Gaps = 2/424 (0%) Query: 7 PSQGLSGEICVPGDKSISHRAVLLAAIAEGQTQVDGFLMGADNLAMVSALQQMGASIQVI 66 P LSG I VPGDKSISHR+++L ++AEG T+V+GFL G D LA + A + MG I+ Sbjct: 308 PGGRLSGRIRVPGDKSISHRSIMLGSLAEGVTEVEGFLEGEDALATLQAFRDMGVVIEGP 367 Query: 67 EDENILVVEGVGMTGLQAPPEALDCGNSGTAIRLLSGLLAGQPFNTVLTGDSSLQRRPMK 126 + + GVG+ GL+ P + GNSGT++RLLSGLLA Q F++VLTGD+SL +RPM Sbjct: 368 HHGRV-TIHGVGLHGLKPAPGPIYLGNSGTSMRLLSGLLAAQSFDSVLTGDASLSKRPMS 426 Query: 127 RIIDPLTLMGAKIDS-TGNVPPLKIYGNPRLTGIHYQLPMASAQVKSCLLLAGLYARGKT 185 R+ PL MGA I++ PPL I G L G+ Y +PMASAQVKSCLLLAGLYA GKT Sbjct: 427 RVAKPLREMGAVIETGPEGRPPLTIRGGQSLKGLAYAMPMASAQVKSCLLLAGLYAEGKT 486 Query: 186 CITEPAPSRDHTERLLKHFHYTLQKDKQSICVSGGGKLKANDISIPGDISSAAFFIVAAT 245 + EPAP+RDHTER+L+ F Y + + + V G L A I +PGDISS+AFF+VAA+ Sbjct: 487 VVAEPAPTRDHTERMLRGFGYPVAVEGATASVESGHALAATHIEVPGDISSSAFFLVAAS 546 Query: 246 ITPGSAIRLCRVGVNPTRLGVINLLKMMGADIEVTHYTEKNEEPTADITVRHARLKGIDI 305 I GS + L VGVNPTR GVI++L++MGADI + + E EP AD+ VR A LKGI+I Sbjct: 547 IAEGSELLLEHVGVNPTRTGVIDILRLMGADITLENQREVGGEPVADLRVRAAALKGIEI 606 Query: 306 PPDQVPLTIDEFPVLLIAAAVAQGKTVLRDAAELRVKETDRIAAMVDGLQKLGIAAESLP 365 P VPL IDEFPVL +AAA A+G+TVLR A ELRVKE+DRI M DGL LG+ E Sbjct: 607 PEALVPLAIDEFPVLFVAAACAEGRTVLRGAQELRVKESDRIQVMADGLLALGVKCEPTA 666 Query: 366 DGVIIQGGTLEGGEVNSYDDHRIAMAFAVAGTLAKGPVRIRNCDNVKTSFPNFVELANEV 425 DG+II GG + GGEV+++ DHRIAMAF+VA A P+RIR+C NV TSFPNF+ L +V Sbjct: 667 DGIIIDGGLIGGGEVHAHGDHRIAMAFSVASLRAATPIRIRDCANVATSFPNFLTLCAQV 726 Query: 426 GMNV 429 G+ V Sbjct: 727 GIRV 730 Lambda K H 0.318 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 903 Number of extensions: 43 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 438 Length of database: 736 Length adjustment: 36 Effective length of query: 402 Effective length of database: 700 Effective search space: 281400 Effective search space used: 281400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
Align candidate GFF1599 PS417_08135 (3-phosphoshikimate 1-carboxyvinyltransferase)
to HMM TIGR01356 (aroA: 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01356.hmm # target sequence database: /tmp/gapView.2911.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01356 [M=415] Accession: TIGR01356 Description: aroA: 3-phosphoshikimate 1-carboxyvinyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2e-128 414.7 0.0 2.7e-128 414.2 0.0 1.2 1 lcl|FitnessBrowser__WCS417:GFF1599 PS417_08135 3-phosphoshikimate 1 Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF1599 PS417_08135 3-phosphoshikimate 1-carboxyvinyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 414.2 0.0 2.7e-128 2.7e-128 1 413 [. 314 727 .. 314 729 .. 0.96 Alignments for each domain: == domain 1 score: 414.2 bits; conditional E-value: 2.7e-128 TIGR01356 1 geikipgsKSishRalllaaLaegetvvtnlLkseDtlatlealrklGakve.eekeelviegvgg..lkepeae 72 g+i++pg+KSishR+++l++Laeg t+v+++L++eD latl+a+r++G+ +e ++++++i+gvg lk + lcl|FitnessBrowser__WCS417:GFF1599 314 GRIRVPGDKSISHRSIMLGSLAEGVTEVEGFLEGEDALATLQAFRDMGVVIEgPHHGRVTIHGVGLhgLKPAPGP 388 79**************************************************666*********9876666669* PP TIGR01356 73 ldlgnsGttaRlltgvlalasgevvltgdeslkkRPierlveaLrelgaeieskeeegslPlaisgplkg.give 146 ++lgnsGt++Rll+g+la++s+++vltgd sl+kRP+ r+ ++Lre+ga ie eg++Pl+i+g++++ g + lcl|FitnessBrowser__WCS417:GFF1599 389 IYLGNSGTSMRLLSGLLAAQSFDSVLTGDASLSKRPMSRVAKPLREMGAVIETGP-EGRPPLTIRGGQSLkGLAY 462 ****************************************************876.69*********8888**** PP TIGR01356 147 lsgsaSsQyksalllaaplalqavtleivgeklisrpyieitLkllksfgveveeederkivvkggqkykqkeve 221 ++aS+Q+ks+llla+ l a++ ++v e+ +r+++e++L ++ v +e + +v+ g++ + +++e lcl|FitnessBrowser__WCS417:GFF1599 463 AMPMASAQVKSCLLLAG---LYAEGKTVVAEPAPTRDHTERMLRGFGYP---VAVEGA-TASVESGHALAATHIE 530 *****************...778899****************9998876...777665.889**999999999** PP TIGR01356 222 vegDaSsAafflaaaaitge.evtvenlgenstqgdkaiiivLeemGadveveeqr........dvevegasklk 287 v+gD+Ss affl+aa i+++ e+ +e++g n+t+++ +i++L+ mGad+++e+qr d++v+ a lk lcl|FitnessBrowser__WCS417:GFF1599 531 VPGDISSSAFFLVAASIAEGsELLLEHVGVNPTRTG--VIDILRLMGADITLENQRevggepvaDLRVR-AAALK 602 *******************99***************..788****************************.789** PP TIGR01356 288 gvkv.didvdsliDelptlavlaafAegetriknieelRvkEsdRiaaiaeeLeklGveveeledgllieGkkke 361 g+++ ++ v+ +iDe+p+l v+aa+Aeg t++++++elRvkEsdRi+++a+ L +lGv++e+++dg++i G+ lcl|FitnessBrowser__WCS417:GFF1599 603 GIEIpEALVPLAIDEFPVLFVAAACAEGRTVLRGAQELRVKESDRIQVMADGLLALGVKCEPTADGIIIDGG--L 675 ****99******************************************************************..6 PP TIGR01356 362 lkgavvdtydDHRiamalavlglaaegeveiedaecvaksfPeFfevleqlg 413 + g++v+ ++DHRiama++v++l+a+ +++i d + va+sfP+F+ + +q+g lcl|FitnessBrowser__WCS417:GFF1599 676 IGGGEVHAHGDHRIAMAFSVASLRAATPIRIRDCANVATSFPNFLTLCAQVG 727 99********************************************999987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (415 nodes) Target sequences: 1 (736 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 11.64 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory