Alignments for a candidate for gatA in Pseudomonas simiae WCS417

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Pseudomonas simiae WCS417

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (uncharacterized)
to candidate GFF3456 PS417_17690 amidase

Query= curated2:Q6A8P5
         (501 letters)



>FitnessBrowser__WCS417:GFF3456
          Length = 467

 Score =  196 bits (498), Expect = 1e-54
 Identities = 159/482 (32%), Positives = 224/482 (46%), Gaps = 43/482 (8%)

Query: 1   MNELLTLTAAELGERIAARQISSEEVTQAHLDRISEVDGDIHAFLLVDHAGALDAARRID 60
           M+E+  LTA +L +    + +S  +VT+  L RI   +  ++A+  VD  GAL+AAR  +
Sbjct: 1   MSEIGQLTAVQLLQHFRDKTLSPVDVTEDALLRIERYNPVVNAYCHVDPEGALNAARASE 60

Query: 61  ARIADGEHLGPLAGVPLAVKDLFCTKGIATTASSQMLEGWIP-PYDSTIVTRCKDAGMVI 119
            R  +G+  G L GVP ++KDL  T G+ T   S+      P   D+      + AG V+
Sbjct: 61  QRWLNGQPCGALDGVPASIKDLTLTVGMPTRKGSRTTSAEGPWDVDAPFTAFMRKAGAVL 120

Query: 120 LGKTNLDEFAMGSSTETSAFGPTHNPWDLERVPGGSGGGSAASLASFQAPLALGTDTGGS 179
           LGKT   EF     T+   +G T NPWD     GGS GG+ A+ A     L  G+D GGS
Sbjct: 121 LGKTTTPEFGWKGVTDNPLYGITRNPWDTRTTAGGSSGGAGAAAALNLGVLHQGSDAGGS 180

Query: 180 IRQPGAVTGTVGIKPTYGSTSRYGVIAMASSLDTPGPCARTVLDAALLHQAIAGHDAMDQ 239
           IR P A TGT GIKPT+G   ++   AM + L   GP  R V D+ L+ Q IA  DA D 
Sbjct: 181 IRIPCAFTGTFGIKPTFGYVPQWPASAM-TLLSHLGPMTRKVEDSVLMLQTIAQPDARD- 238

Query: 240 TTINQP--TPAVVEAARQTDVSGVRIGVVTELSGQVYDPQVEARFHEAVEMLIEAGAEVV 297
             I  P  TP +      TD++G+R+           DPQV     +AV  L++ GA V 
Sbjct: 239 GLIGAPRTTPWLTPG---TDLNGLRVAYSPNFGYVDVDPQVAKVVAQAVAGLVQLGAHVE 295

Query: 298 EVSCPNFDLALPAYYLIQPAEVSSNLARYDAMRYGLRVNDDGEHSAEQ--VMRATRGAGL 355
           ++  P F           P EV S L    A R    +ND  +   +   +  A RG  L
Sbjct: 296 QID-PGFS---------DPLEVFSTLWAAGAARLTRPMNDAQKQLLDPGLLRIAQRGERL 345

Query: 356 GAEAKRRIILGTYALSAGYYDAYYGSAQKVRTLIQRDFEKAWQMCDVLVSPATPTTAFRL 415
             +                    + +A + R  +        +  DVLVSP  P TAF  
Sbjct: 346 SLDD-------------------FNAALEARAALVARMAAFHEHYDVLVSPMMPITAFDA 386

Query: 416 GERTADPMAMY---RSDLCTVPANMAGSPAGSFPIGLSETDGMPVGMQVMAPIMADDRIY 472
           G        +    +    T P N+   PA S P GL+ T+G+PVG+ V+    AD+++ 
Sbjct: 387 GHDVPPGSGLQEWTQWTPFTYPFNLTQQPAASVPCGLA-TNGLPVGLHVVGARFADEQVL 445

Query: 473 RV 474
           RV
Sbjct: 446 RV 447


Lambda     K      H
   0.317    0.133    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 564
Number of extensions: 35
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 501
Length of database: 467
Length adjustment: 34
Effective length of query: 467
Effective length of database: 433
Effective search space:   202211
Effective search space used:   202211
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis