Align histidinol-phosphate aminotransferase (EC 2.6.1.9) (characterized)
to candidate GFF872 PS417_04425 histidinol-phosphate aminotransferase
Query= reanno::pseudo6_N2E2:Pf6N2E2_3251 (350 letters) >FitnessBrowser__WCS417:GFF872 Length = 350 Score = 624 bits (1609), Expect = 0.0 Identities = 309/350 (88%), Positives = 329/350 (94%) Query: 1 MSKFWSPFVKNLVPYVPGEQPKLTRLVKLNTNENPYGPSPKALAAMQTELNDNLRLYPDP 60 MSKFWSPFVK+LVPYVPGEQPKLT+LVKLNTNENPYGPSPKALAAMQ ELNDNLRLYPDP Sbjct: 1 MSKFWSPFVKDLVPYVPGEQPKLTKLVKLNTNENPYGPSPKALAAMQAELNDNLRLYPDP 60 Query: 61 NSDLLKGAVARYYGVQSNQVFLGNGSDEVLAHIFHGLLQHDQPLLFPDISYSFYPVYCGL 120 NSDLLK AVA+YYG+ + +VFLGNGSDEVLAHIFHGL QHD PLLFPDISYSFYPVYCGL Sbjct: 61 NSDLLKQAVAKYYGIDAGKVFLGNGSDEVLAHIFHGLFQHDLPLLFPDISYSFYPVYCGL 120 Query: 121 YGIQFDAVPLDAQFQIDPADYAKPNGGIIFPNPNAPTGCLLALEAVEQILKANPDSVVVV 180 YGIQ D VPLD QFQI ADYAKPNGGIIFPNPNAPTG +LAL+AVEQILKA+PDSVVVV Sbjct: 121 YGIQSDPVPLDEQFQIRVADYAKPNGGIIFPNPNAPTGTVLALDAVEQILKASPDSVVVV 180 Query: 181 DEAYIDFGGETAISLVDRYPNLLVTQTLSKSRSLAGLRVGLAVGHPELIEALERIKNSFN 240 DEAYIDFGGETAISLVDRYPNLLVTQTLSKSRSLAGLRVGLAVGHP+LIEALER+KNSFN Sbjct: 181 DEAYIDFGGETAISLVDRYPNLLVTQTLSKSRSLAGLRVGLAVGHPDLIEALERVKNSFN 240 Query: 241 SYPLDRLANVGGAAAFDDREYFDRTCRLVIEHREWVVAQLQAKGFEVLPSAANFIFARHP 300 SYPLDRLA VG AAAF+DREYF++TC+ VI+ R +VAQL+ KGFEVLPSAANFIFARHP Sbjct: 241 SYPLDRLAIVGAAAAFEDREYFEKTCQWVIDSRNTLVAQLEEKGFEVLPSAANFIFARHP 300 Query: 301 RHDAAGLAAKLREQGVIVRHFKQERIAQFLRISIGTPEQNQALIEALGEL 350 +DAAGLAAKLREQGVIVRHFKQERIAQFLRISIGTPEQNQALI+ LGEL Sbjct: 301 EYDAAGLAAKLREQGVIVRHFKQERIAQFLRISIGTPEQNQALIDGLGEL 350 Lambda K H 0.320 0.139 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 565 Number of extensions: 14 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 350 Length of database: 350 Length adjustment: 29 Effective length of query: 321 Effective length of database: 321 Effective search space: 103041 Effective search space used: 103041 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate GFF872 PS417_04425 (histidinol-phosphate aminotransferase)
to HMM TIGR01141 (hisC: histidinol-phosphate transaminase (EC 2.6.1.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01141.hmm # target sequence database: /tmp/gapView.23521.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01141 [M=349] Accession: TIGR01141 Description: hisC: histidinol-phosphate transaminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.9e-95 304.3 0.0 5.5e-95 304.1 0.0 1.0 1 lcl|FitnessBrowser__WCS417:GFF872 PS417_04425 histidinol-phosphate Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF872 PS417_04425 histidinol-phosphate aminotransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 304.1 0.0 5.5e-95 5.5e-95 3 348 .. 8 349 .. 6 350 .] 0.94 Alignments for each domain: == domain 1 score: 304.1 bits; conditional E-value: 5.5e-95 TIGR01141 3 kikklepYqpgarelgekevvkLnsnEnPfgpsekvkealkeelk.klhrYpdpqalelkealakylgveeenill 77 +k+l pY+pg++ + +k vkLn+nEnP+gps+k+++a+++el+ +l++Ypdp++ lk+a+aky+g+++ +++l lcl|FitnessBrowser__WCS417:GFF872 8 FVKDLVPYVPGEQPKLTK-LVKLNTNENPYGPSPKALAAMQAELNdNLRLYPDPNSDLLKQAVAKYYGIDAGKVFL 82 699*******98877775.*************************99****************************** PP TIGR01141 78 gnGsdelielliraflepgdavlvleptysmYevsakiagaevkevplkedgqedleavleaakekvklvflasPn 153 gnGsde++ +++ + + l+++ +ys Y+v+ ++g++ vpl+e++q +++ + k + ++f +Pn lcl|FitnessBrowser__WCS417:GFF872 83 GNGSDEVLAHIFHGLFQHDLPLLFPDISYSFYPVYCGLYGIQSDPVPLDEQFQIRVADYA---KPNGGIIF-PNPN 154 ***************************************************977777776...55555555.89** PP TIGR01141 154 nPtGnllkreeiekvleevedalVVvDeAYieFseeasvlellaeypnlvvlrTlSKafgLAglRvGyaianaeii 229 +PtG++l + +e++l+++ d +VVvDeAYi+F +e ++++l+ +ypnl+v++TlSK+ +LAglRvG+a++++++i lcl|FitnessBrowser__WCS417:GFF872 155 APTGTVLALDAVEQILKASPDSVVVVDEAYIDFGGE-TAISLVDRYPNLLVTQTLSKSRSLAGLRVGLAVGHPDLI 229 ******************98***************7.*************************************** PP TIGR01141 230 ealekvrapyn...vsslaleaavaalrdsdkiektveevkkererlleelkkleglevyeSkaNFvlikvke.da 301 eale+v++++n +++la a aa +d++++ekt + v+ +r+ l ++l++ g+ev +S aNF++++ +e da lcl|FitnessBrowser__WCS417:GFF872 230 EALERVKNSFNsypLDRLAIVGAAAAFEDREYFEKTCQWVIDSRNTLVAQLEEK-GFEVLPSAANFIFARHPEyDA 304 *******87543339*************************************99.8****************99** PP TIGR01141 302 eelleallekgiivRdlksaeglleeclRitvGtreenerllealke 348 + l+ +l e+g+ivR++k+ + ++lRi++Gt+e+n++l++ l e lcl|FitnessBrowser__WCS417:GFF872 305 AGLAAKLREQGVIVRHFKQE--RIAQFLRISIGTPEQNQALIDGLGE 349 *****************977..48******************98876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (349 nodes) Target sequences: 1 (350 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 8.18 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory