GapMind for Amino acid biosynthesis

 

Aligments for a candidate for dapC in Pseudomonas simiae WCS417

Align succinyldiaminopimelate transaminase (EC 2.6.1.17) (characterized)
to candidate GFF4517 PS417_23120 aminotransferase

Query= BRENDA::P9WPZ5
         (397 letters)



>lcl|FitnessBrowser__WCS417:GFF4517 PS417_23120 aminotransferase
          Length = 382

 Score =  256 bits (654), Expect = 8e-73
 Identities = 153/393 (38%), Positives = 214/393 (54%), Gaps = 13/393 (3%)

Query: 1   MTVSRLRPYATTVFAEMSALATRIGAVNLGQGFPDEDGPPKMLQAAQDAIAGGVNQYPPG 60
           M  S+L    TT+F  MS LA   GA+NL QGFPD  GP  +L A    IA G NQY P 
Sbjct: 1   MITSKLPNVGTTIFTTMSQLAAETGALNLSQGFPDFSGPQGLLDAVGKHIALGHNQYAPM 60

Query: 61  PGSAPLRRAIAAQRRRHFGVDYDPETEVLVTVGATEAIAAAVLGLVEPGSEVLLIEPFYD 120
            G   LR+ +AA+  R +G   D ++EV +T GATEAI  A+  ++  G EV++ +P YD
Sbjct: 61  TGLPVLRQQVAAKIARSYGATVDADSEVTITPGATEAIFCAIQAVIRNGDEVIVFDPSYD 120

Query: 121 SYSPVVAMAGAHRVTVPLVPDGRGFALDADALRRAVTPRTRALIINSPHNPTGAVLSATE 180
           SY P V +AG   V V L   G  FALD + ++ A++PRTR +I+N+PHNPTGA++S  E
Sbjct: 121 SYEPSVELAGGRCVHVQLSLSG--FALDFEKIKAALSPRTRMIILNTPHNPTGALISRAE 178

Query: 181 LAAIAEIAVAANLVVITDEVYEHLVFDHARHLPLAGFDGMAERTITISSAAKMFNCTGWK 240
           L  +AE+    ++ +++DEVYEHLVFD   H+ +   + +  R   +SS  K ++ TGWK
Sbjct: 179 LDQLAELIRDRDIYLVSDEVYEHLVFDGVAHVSVLAHEELYPRAFVVSSFGKTYHVTGWK 238

Query: 241 IGWACGPAELIAGVRAAKQYLSYVGGAPFQPAVALALDTEDAWVAALRNSLRARRDRLAA 300
            G+   P  L A +R   QY+++ G  P Q A+A  +      V  L    +A+RD    
Sbjct: 239 TGYVVAPPALTAELRKVHQYVNFCGVTPLQYALADFMAEHPEHVEELPAFYQAKRDLFCD 298

Query: 301 GLTEIGFAVHDSYGTYFLCADPRPLGYD-DSTEFCAALPEKVGVAAIPMSAFCD-PAAGQ 358
            L    F+     GTYF   D   +  D D       +  + GVA IP+S F   P  GQ
Sbjct: 299 LLAASRFSFTRVTGTYFQLVDYSQIRPDLDDVAMSLWMTREHGVATIPVSVFYQTPPQGQ 358

Query: 359 ASQQADVWNHLVRFTFCKRDDTLDEAIRRLSVL 391
                     LVR  F KR++TL +A  +L V+
Sbjct: 359 ---------RLVRLCFAKREETLRQAAEKLCVI 382


Lambda     K      H
   0.321    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 362
Number of extensions: 16
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 397
Length of database: 382
Length adjustment: 30
Effective length of query: 367
Effective length of database: 352
Effective search space:   129184
Effective search space used:   129184
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory