Alignments for a candidate for serB in Pseudomonas simiae WCS417

GapMind for Amino acid biosynthesis

Alignments for a candidate for serB in Pseudomonas simiae WCS417

Align fused D-3-phosphoglycerate dehydrogenase / phosphoserine phosphatase (EC 1.1.1.95; EC 3.1.3.3) (characterized)
to candidate GFF5281 PS417_27040 3-phosphoglycerate dehydrogenase

Query= reanno::Cola:Echvi_2777
         (630 letters)



>FitnessBrowser__WCS417:GFF5281
          Length = 409

 Score =  443 bits (1139), Expect = e-129
 Identities = 218/407 (53%), Positives = 300/407 (73%), Gaps = 1/407 (0%)

Query: 225 KFSYPKSRINVLLLENVHPIGVEIMKQEGY-NVEVVSSAMSEEELCEKIKNVSIIGIRSK 283
           K S  KS+I  LLLE VHP  V+++K  GY ++E ++S++ E +L EKI +   IGIRS+
Sbjct: 3   KTSLDKSKIKFLLLEGVHPSAVDVLKAAGYTSIEYITSSLPEAQLKEKIADAHFIGIRSR 62

Query: 284 TQITKKVLENANRLMAVGAFCIGTNQIDLETCQEKGIAVFNAPFSNTRSVVELAISEIIF 343
           TQ+T+++ ++A +L+AVG FCIGTNQ+DL   +E+GIAVFNAP+SNTRSV EL ++E I 
Sbjct: 63  TQLTEEIFDHAKKLVAVGCFCIGTNQVDLNAARERGIAVFNAPYSNTRSVAELVLAEAIL 122

Query: 344 LMRNLHDKTLKMHQGIWNKSASGSFEVRGKKLGIIGYGNIGAQLSVLAENMGMNVFYYDI 403
           L+R + +K    H+G W KSA+ SFE+RGKKLGI+GYG+IG QLSVLAE +GM VF+YD 
Sbjct: 123 LLRGIPEKNASCHRGGWIKSAANSFEIRGKKLGIVGYGSIGTQLSVLAEGLGMQVFFYDT 182

Query: 404 VERLALGNATKIDSLDELLETCDIISLHVDGRTENKNILNKEKIFKMKKGAILVNLSRGH 463
           + +L LGNAT++ SL ELL   DI++LHV    E + ++ +++I  +KKG IL+N +RG 
Sbjct: 183 LTKLPLGNATQVASLTELLGMSDIVTLHVPETAETQWMIGEKEIRAIKKGGILINAARGT 242

Query: 464 VVDVPALRDALESGHLAGAAVDVFPTEPKNNDEPFESELIGCPNTILTPHIGGSTLEAQE 523
           VV++ AL DA++  HL GAA+DVFP EP++ND+ FES L G  N ILTPHIGGST EAQ 
Sbjct: 243 VVELDALADAIKDKHLIGAAIDVFPEEPRSNDDIFESPLRGLDNVILTPHIGGSTAEAQA 302

Query: 524 NIAQFVPGKIIEYINSGNTFNSVNFPNIQLPFLKDAHRLIHIHQNAPGVLAKINQVLASY 583
           NI   V  K+++Y ++G + +SVNFP + LP     HRL+HIHQN PGV+ +IN+V A  
Sbjct: 303 NIGLEVSEKLVKYSDNGTSVSSVNFPEVALPAHPGKHRLLHIHQNIPGVMMEINKVFAEN 362

Query: 584 KINIVGQYLKTNEKIGYVITDIDKRYSNDVIDALKEIEGTIRFRILY 630
            INI GQ+L+TNEK+GYV+ D+D  YS+   + L+ I GTIR R+L+
Sbjct: 363 GINISGQFLQTNEKVGYVVIDVDAEYSDLAQEKLQHINGTIRSRVLF 409


Lambda     K      H
   0.317    0.136    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 632
Number of extensions: 27
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 630
Length of database: 409
Length adjustment: 34
Effective length of query: 596
Effective length of database: 375
Effective search space:   223500
Effective search space used:   223500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis