Align Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 (characterized)
to candidate Ac3H11_1527 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)
Query= SwissProt::Q58667 (170 letters) >FitnessBrowser__acidovorax_3H11:Ac3H11_1527 Length = 221 Score = 74.7 bits (182), Expect = 1e-18 Identities = 52/157 (33%), Positives = 80/157 (50%), Gaps = 29/157 (18%) Query: 12 DDVDTDAIIPGPYLRTT----------DPYELASHCMAGIDENFPK----------KVKE 51 ++VDTDAIIP +L++ D + GI E+ K + K Sbjct: 17 ENVDTDAIIPKQFLKSIKKTGFGPNLFDEWRYLDKGEPGIPESARKPNPDFVLNLPRYKG 76 Query: 52 GDVIVAGENFGCGSSREQAVIAIKYCGIKAVIAKSFARIFYRNAINVGLIPII---ANTD 108 +++A +NFGCGSSRE A A+ G +AVIA SFA IF+ N GL+PI+ A Sbjct: 77 ASILIARKNFGCGSSREHAPWALDQFGFRAVIAPSFADIFFNNCFKNGLLPIVLPEATVA 136 Query: 109 EIKD------GDIVEIDLDKEEIVITNKNKTIKCETP 139 ++ D G + IDL+++ +V N+++ + E P Sbjct: 137 QLFDEVAAFPGYQLTIDLERQVVVRQNRDEVVGEEIP 173 Lambda K H 0.318 0.139 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 125 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 170 Length of database: 221 Length adjustment: 20 Effective length of query: 150 Effective length of database: 201 Effective search space: 30150 Effective search space used: 30150 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 44 (21.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory