Alignments for a candidate for metH in Acidovorax sp. GW101-3H11

GapMind for Amino acid biosynthesis

Alignments for a candidate for metH in Acidovorax sp. GW101-3H11

Align methionine synthase; EC 2.1.1.13 (characterized)
to candidate Ac3H11_3274 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)

Query= CharProtDB::CH_090726
         (1227 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_3274
          Length = 903

 Score = 1052 bits (2721), Expect = 0.0
 Identities = 566/908 (62%), Positives = 666/908 (73%), Gaps = 32/908 (3%)

Query: 341  RLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVENGAQIIDI 400
            +LSGLEP++IGE +LFVN+GERTNVTGS  F R+I   +Y EAL VARQQVENGAQ+IDI
Sbjct: 2    KLSGLEPVSIGEGTLFVNIGERTNVTGSKAFARMILNGQYEEALAVARQQVENGAQVIDI 61

Query: 401  NMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQGKGIVNSISMK 460
            NMDE MLD++AAMVRFL LIA EPDIARVPIM+DSSKWDVIE GL+CIQGKGIVNSISMK
Sbjct: 62   NMDEAMLDSKAAMVRFLQLIASEPDIARVPIMVDSSKWDVIEAGLRCIQGKGIVNSISMK 121

Query: 461  EGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKILTEEVGFPPEDII 520
            EGV+ F H A+L+RRYGAA VVMAFDE GQADT ARKIEIC RAY++L +EVGFPPEDII
Sbjct: 122  EGVEKFKHEARLVRRYGAAAVVMAFDEVGQADTYARKIEICERAYRVLVDEVGFPPEDII 181

Query: 521  FDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVSFSFRGNDPVREAIH 580
            FDPNIFAVATGIEEHNNYA DFI A   IK+ LP A +SGGVSNVSFSFRGNDPVREAIH
Sbjct: 182  FDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPGAKVSGGVSNVSFSFRGNDPVREAIH 241

Query: 581  AVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRRDDGTERLLELAEKYRG 640
             VFLY+AI+ GMDMGIVNAG + +YDDL   LR+ VEDV+LNRR D  ERL+E+AE  + 
Sbjct: 242  TVFLYHAIQAGMDMGIVNAGMVGVYDDLEPVLRERVEDVVLNRRADAGERLVEIAETAKS 301

Query: 641  SKTDDTANAQQAEWRSW-----EVNKRLEYSLVKGITEFIEQDTEEARQ----QATRPIE 691
               D+   +++ EWR        V +RL ++LV GIT+FI +DTEEA Q    +  RP+ 
Sbjct: 302  GAKDE---SKKLEWRGTPEHPKTVGERLSHALVHGITDFIVEDTEEAYQAIVAKGGRPLH 358

Query: 692  VIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASKEQG-------K 744
            VIEGPLMDGMNVVGDLFG GKMFLPQVVKSARVMK AVA+L P+IE  K Q        +
Sbjct: 359  VIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKSAVAHLIPYIEEEKRQDELAGRDVR 418

Query: 745  TNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNADLIGLS 804
            + GK++IATVKGDVHDIGKNIV VVLQCNN+E+V++GVMVP  +IL  AK   AD++GLS
Sbjct: 419  SKGKIIIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCHEILARAKVEGADIVGLS 478

Query: 805  GLITPSLDEMVNVAKEMERQGF----TIPLLIGGATTSKAHTAVKIEQNYSGPTVYVQNA 860
            GLITPSL+EM  VA EM++        IPLLIGGATTS+ HTAVKI  +Y GP VYV +A
Sbjct: 479  GLITPSLEEMQYVAGEMQKDDHFRIKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDA 538

Query: 861  SRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDFAFDWQA 920
            SR+V V  +LL D  + ++V     +YE VR QH  KK +TP  +LE AR N    ++  
Sbjct: 539  SRSVSVAQSLLGDGVQ-NYVQELNADYEKVRTQHANKK-QTPMWSLEKARANRTPVNFST 596

Query: 921  YTPPVAHRLGVQEVE-ASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVEAQRLFKD 979
            Y PP    LG +  +   +  L  YIDW PFF TW LAG YP IL DEVVGVEA R+F D
Sbjct: 597  YVPPTPRLLGRRVFKNFDLAELAKYIDWGPFFQTWDLAGPYPAILTDEVVGVEATRVFAD 656

Query: 980  ANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLRQQTEKTGF- 1038
               ML KL   + L+  GV+ L+PAN VGDDIE Y DETRT V    + +RQQTEK    
Sbjct: 657  GQAMLKKLIEGRWLSASGVMALYPANSVGDDIEFYTDETRTQVAMTWYGMRQQTEKQMID 716

Query: 1039 ----ANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMVKALADR 1094
                 + CLADFVAPK SG ADY G FAVT GL  +    AF    DDY+ IM+K+LADR
Sbjct: 717  GVMRPSRCLADFVAPKDSGIADYAGLFAVTAGLGVEKKEQAFVDALDDYSAIMLKSLADR 776

Query: 1095 LAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHTEKATIWEL 1154
            LAEAFAE LH+RVR   WGYA  E LSN+E+I E Y GIRPAPGYPACP+H+ K  ++ +
Sbjct: 777  LAEAFAECLHQRVRTDLWGYAAGEQLSNDEMIAEKYAGIRPAPGYPACPDHSAKQELFRV 836

Query: 1155 LEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYARRKGMSVTEV 1214
            L+ E+  GM LTES AM P ASVSG+Y  HPDS Y+ V +I  DQ+ D A R+GM    +
Sbjct: 837  LQCEE-IGMGLTESLAMTPAASVSGFYIGHPDSVYFNVGKIGEDQLHDMAERRGMDEKVL 895

Query: 1215 ERWLAPNL 1222
            ER LAPNL
Sbjct: 896  ERLLAPNL 903


Lambda     K      H
   0.318    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2674
Number of extensions: 118
Number of successful extensions: 11
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 1227
Length of database: 903
Length adjustment: 45
Effective length of query: 1182
Effective length of database: 858
Effective search space:  1014156
Effective search space used:  1014156
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)

Align methionine synthase; EC 2.1.1.13 (characterized)
to candidate Ac3H11_3297 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)

Query= CharProtDB::CH_090726
         (1227 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_3297
          Length = 354

 Score =  371 bits (953), Expect = e-107
 Identities = 186/344 (54%), Positives = 253/344 (73%), Gaps = 15/344 (4%)

Query: 3   SKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGE----------RFADWPCDLKGN 52
           ++  QL   L +RI++LDG MGTMIQ ++L EA +RGE          RF D+  D+KGN
Sbjct: 7   TRAAQLPDILAQRIVILDGAMGTMIQRFKLGEAQYRGEGYTGPGKAGDRFKDFAYDVKGN 66

Query: 53  NDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAEINFAAAKLAR 112
           N+LL L++P+VI+ IH  Y  AGAD+IETNTF +TTIA  DY M +L+ E+N  +A+LAR
Sbjct: 67  NELLSLTRPDVISDIHERYLAAGADLIETNTFGATTIAQEDYHMANLAREMNLRSAELAR 126

Query: 113 ACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLVAAYRESTKALVE 172
           A  D+++  TP+KPR+VAG LGPT +TASISPDVNDP  RN+ F+ L AAY E T+ALVE
Sbjct: 127 AACDKYS--TPDKPRFVAGALGPTPKTASISPDVNDPGARNVDFEALRAAYYEQTEALVE 184

Query: 173 GGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDASGRTLSGQTTEAFY 232
           GG+D++L+ET+FDTLNAKAA+FA+   FE  G  LP++ISGT+TDASGR LSGQT  AF+
Sbjct: 185 GGSDVLLVETIFDTLNAKAALFAIDEYFENSGQRLPLIISGTVTDASGRILSGQTVTAFW 244

Query: 233 NSLRHAEALTFGLNCALGPDELRQYVQELSRIAE-CYVTAHPNAGLPNAFGE--YDLDAD 289
           +S+RH+  L  GLNCALG   +R Y+QEL+R+AE  +++ +PNAGLPN   +  +D   +
Sbjct: 245 HSVRHSRPLAIGLNCALGATLMRPYIQELNRVAEDTFISCYPNAGLPNPMSDTGFDETPE 304

Query: 290 TMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKL 333
             ++ + E+A  G +NI+GGCCGTTP HI A+++AVE +  RK+
Sbjct: 305 ITSRLVHEFAAEGLVNILGGCCGTTPDHIGAIAKAVESVPTRKM 348


Lambda     K      H
   0.318    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1007
Number of extensions: 32
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 1227
Length of database: 354
Length adjustment: 38
Effective length of query: 1189
Effective length of database: 316
Effective search space:   375724
Effective search space used:   375724
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

Align candidate Ac3H11_3274 (5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13))
to HMM TIGR02082 (metH: methionine synthase (EC 2.1.1.13))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR02082.hmm
# target sequence database:        /tmp/gapView.26825.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02082  [M=1182]
Accession:   TIGR02082
Description: metH: methionine synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                        Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                        -----------
          0 1191.4   0.0          0 1191.2   0.0    1.0  1  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  5-methyltetrahydrofolate--homocy


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 ! 1191.2   0.0         0         0     328    1182 .]       2     873 ..       1     873 [. 0.96

  Alignments for each domain:
  == domain 1  score: 1191.2 bits;  conditional E-value: 0
                                        TIGR02082  328 vlsglealkiaqessfvniGeRtnvaGskkfrklikaedyeealkiakqqveeGaqilDi 387 
                                                        lsgle+++i++ + fvniGeRtnv+Gsk f+++i +++yeeal +a+qqve+Gaq++Di
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274    2 KLSGLEPVSIGEGTLFVNIGERTNVTGSKAFARMILNGQYEEALAVARQQVENGAQVIDI 61  
                                                       68********************************************************** PP

                                        TIGR02082  388 nvDevllDgeadmkkllsllasepdiakvPlmlDssefevleaGLkviqGkaivnsislk 447 
                                                       n+De++lD++a+m+++l+l+asepdia+vP+m+Dss+++v+eaGL++iqGk+ivnsis+k
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274   62 NMDEAMLDSKAAMVRFLQLIASEPDIARVPIMVDSSKWDVIEAGLRCIQGKGIVNSISMK 121 
                                                       ************************************************************ PP

                                        TIGR02082  448 dGeerFlekaklikeyGaavvvmafDeeGqartadkkieiakRayklltekvgfppedii 507 
                                                       +G+e+F ++a+l+++yGaa+vvmafDe Gqa+t+++kiei++Ray++l+++vgfppedii
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  122 EGVEKFKHEARLVRRYGAAAVVMAFDEVGQADTYARKIEICERAYRVLVDEVGFPPEDII 181 
                                                       ************************************************************ PP

                                        TIGR02082  508 fDpniltiatGieehdryaidfieaireikeelPdakisgGvsnvsFslrgndavRealh 567 
                                                       fDpni+++atGieeh++ya+dfiea+r+ik++lP ak+sgGvsnvsFs+rgnd+vRea+h
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  182 FDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPGAKVSGGVSNVSFSFRGNDPVREAIH 241 
                                                       ************************************************************ PP

                                        TIGR02082  568 svFLyeaikaGlDmgivnagklavyddidkelrevvedlildrrreatekLlelaelykg 627 
                                                       +vFLy+ai+aG+Dmgivnag++ vydd+++ lre ved++l+rr +a e+L+e+ae+ k 
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  242 TVFLYHAIQAGMDMGIVNAGMVGVYDDLEPVLRERVEDVVLNRRADAGERLVEIAETAKS 301 
                                                       *********************************************************776 PP

                                        TIGR02082  628 tkeksskeaqeaewrnlp.....veeRLeralvkGeregieedleear....kklkaple 678 
                                                         +++   +++ ewr++p     v eRL++alv+G++++i ed+eea+     k  +pl+
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  302 GAKDE---SKKLEWRGTPehpktVGERLSHALVHGITDFIVEDTEEAYqaivAKGGRPLH 358 
                                                       66664...44666666543333389***********************444446689*** PP

                                        TIGR02082  679 iiegpLldGmkvvGdLFGsGkmfLPqvvksarvmkkavayLePylekekeed.......k 731 
                                                       +iegpL+dGm+vvGdLFG+GkmfLPqvvksarvmk ava+L+Py+e+ek +d       +
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  359 VIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKSAVAHLIPYIEEEKRQDelagrdvR 418 
                                                       *************************************************9999******* PP

                                        TIGR02082  732 skGkivlatvkGDvhDiGknivdvvLscngyevvdlGvkvPvekileaakkkkaDvigls 791 
                                                       skGki++atvkGDvhDiGkniv vvL+cn++evv++Gv+vP+++il  ak + aD++gls
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  419 SKGKIIIATVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVPCHEILARAKVEGADIVGLS 478 
                                                       ************************************************************ PP

                                        TIGR02082  792 GLivksldemvevaeemerrg.vk...iPlllGGaalskahvavkiaekYkgevvyvkda 847 
                                                       GLi++sl+em++va em++   ++   iPll+GGa++s+ h+avkia++Y+g+vvyv da
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  479 GLITPSLEEMQYVAGEMQKDDhFRikkIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDA 538 
                                                       *****************9975333446********************************* PP

                                        TIGR02082  848 seavkvvdkllsekkkaeelekikeeyeeirekfgekkeklialsekaarkevfaldrse 907 
                                                       s++v+v+++ll +   ++++++++++ye++r+++ + k+++   s+++ar ++  ++ s 
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  539 SRSVSVAQSLLGDGV-QNYVQELNADYEKVRTQHAN-KKQTPMWSLEKARANRTPVNFS- 595 
                                                       *************96.89****************98.7789999**********99999. PP

                                        TIGR02082  908 dlevpapkflGtkvleas.ieellkyiDwkalFvqWelrgkypkilkdeleglearklfk 966 
                                                        + +p+p+ lG++v+++  ++el kyiDw ++F +W+l+g yp il+de++g+ea+++f+
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  596 TYVPPTPRLLGRRVFKNFdLAELAKYIDWGPFFQTWDLAGPYPAILTDEVVGVEATRVFA 655 
                                                       9*********************************************************** PP

                                        TIGR02082  967 dakelldklsaekllrargvvGlfPaqsvgddieiytdetvsqetkpiatvrekleqlrq 1026
                                                       d +++l+kl++ + l a+gv+ l+Pa+svgddie ytdet++   ++  t +  ++q+++
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  656 DGQAMLKKLIEGRWLSASGVMALYPANSVGDDIEFYTDETRT---QVAMTWYGMRQQTEK 712 
                                                       ****************************************94...445555555555555 PP

                                        TIGR02082 1027 qsdr.....ylclaDfiaskesGikDylgallvtaglgaeelakkleakeddydsilvka 1081
                                                       q          claDf+a+k+sGi+Dy g+++vtaglg+e+  +++ +  ddy++i++k+
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  713 QMIDgvmrpSRCLADFVAPKDSGIADYAGLFAVTAGLGVEKKEQAFVDALDDYSAIMLKS 772 
                                                       54444667899************************************************* PP

                                        TIGR02082 1082 ladrlaealaellhervRkelwgyaeeenldkedllkerYrGirpafGYpacPdhtekat 1141
                                                       ladrlaea+ae lh+rvR +lwgya+ e+l++++++ e+Y Girpa+GYpacPdh+ k++
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  773 LADRLAEAFAECLHQRVRTDLWGYAAGEQLSNDEMIAEKYAGIRPAPGYPACPDHSAKQE 832 
                                                       ************************************************************ PP

                                        TIGR02082 1142 lleLleaeriGlklteslalaPeasvsglyfahpeakYfav 1182
                                                       l+++l+ e+iG+ ltesla++P+asvsg+y+ hp++ Yf+v
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_3274  833 LFRVLQCEEIGMGLTESLAMTPAASVSGFYIGHPDSVYFNV 873 
                                                       ***************************************98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (1182 nodes)
Target sequences:                          1  (903 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.08u 0.03s 00:00:00.11 Elapsed: 00:00:00.09
# Mc/sec: 11.27
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis