Alignments for a candidate for gatB in Azospirillum brasilense Sp245

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatB in Azospirillum brasilense Sp245

Align Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.- (characterized)
to candidate AZOBR_RS20650 AZOBR_RS20650 glutamyl-tRNA amidotransferase subunit B

Query= SwissProt::Q9RF06
         (475 letters)



>FitnessBrowser__azobra:AZOBR_RS20650
          Length = 483

 Score =  440 bits (1131), Expect = e-128
 Identities = 221/473 (46%), Positives = 324/473 (68%), Gaps = 5/473 (1%)

Query: 3   FETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAMRA 62
           +E VIGLEVH ++ +++K+FS +   FGAEPNS  + +D A+PG+LPV+N+R ++ A+R 
Sbjct: 11  WEIVIGLEVHAQVISNAKLFSGAATAFGAEPNSQVSFVDAAFPGMLPVINERCIEQAVRT 70

Query: 63  AMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEV-DGETKRIGITRL 121
            + L  +I   S FDRKNYFY D P+ YQISQ+ QPI   G I +++ DG ++ +G+TRL
Sbjct: 71  GLGLKAQINLHSVFDRKNYFYADLPQGYQISQYLQPIVGKGEIVLDLPDGSSRTVGVTRL 130

Query: 122 HMEEDAGKSTHKGE--YSLVDLNRQGTPLIEIVSEPDIRSPKEAYAYLEKLRSIIQYTGV 179
           H+E+DAGKS H      + +DLNR G  L+EIVSEPD+R+ +EA AY+ KLRSI++Y G 
Sbjct: 131 HLEQDAGKSLHDQHPAKTYIDLNRSGVALMEIVSEPDMRTAEEAGAYVRKLRSILRYLGT 190

Query: 180 SDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFNYVRKGLEYEEKRQEEELLSGG 239
            D  MEEGS+RCD N+S+R  G   FGT+ E+KN+NS  +V + +EYE +RQ E +  GG
Sbjct: 191 CDGNMEEGSMRCDVNVSVRKPGAP-FGTRCEIKNVNSIRFVMQAIEYEARRQIEIIEEGG 249

Query: 240 EIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKERVRQTIPELPDER 299
           +I QETR +D +   T  MR KE + DYRYFP+PD++PL +D  W E +++T+PELPD++
Sbjct: 250 KIDQETRLWDTTKFVTRSMRSKEEAHDYRYFPDPDLLPLELDADWVEDIKKTLPELPDDK 309

Query: 300 KAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVNEYLNKNQVEL 359
           KA++++E  L  YDA+VL   K  +DF+E+ +  G D KL +NW+ G +  YLNK   E+
Sbjct: 310 KARFISEYKLSPYDANVLVSEKARADFYEA-VAKGRDPKLAANWVTGELFGYLNKAGKEI 368

Query: 360 LDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNGLVQISDEATLL 419
            ++ ++ ENL G+I LI D T+S +IAK+VF  +   G     I+E  GL Q++D   + 
Sbjct: 369 EESPVSAENLGGLIDLIADNTISGRIAKEVFEAMFETGEKPADIVEKKGLRQVTDTGAIE 428

Query: 420 KFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQEL 472
             ++  L  N   V ++++GK K  GF VGQ+MKA++G+ANP LVN++L  +L
Sbjct: 429 SSIDAVLAANADKVAEFRSGKDKLFGFFVGQVMKATQGKANPALVNEILMAKL 481


Lambda     K      H
   0.313    0.133    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 616
Number of extensions: 27
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 475
Length of database: 483
Length adjustment: 34
Effective length of query: 441
Effective length of database: 449
Effective search space:   198009
Effective search space used:   198009
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)

Align candidate AZOBR_RS20650 AZOBR_RS20650 (glutamyl-tRNA amidotransferase subunit B)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00133.hmm
# target sequence database:        /tmp/gapView.18573.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00133  [M=481]
Accession:   TIGR00133
Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   3.4e-168  546.0   0.0   3.9e-168  545.8   0.0    1.0  1  lcl|FitnessBrowser__azobra:AZOBR_RS20650  AZOBR_RS20650 glutamyl-tRNA amid


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__azobra:AZOBR_RS20650  AZOBR_RS20650 glutamyl-tRNA amidotransferase subunit B
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  545.8   0.0  3.9e-168  3.9e-168       3     480 ..      10     481 ..       8     482 .. 0.97

  Alignments for each domain:
  == domain 1  score: 545.8 bits;  conditional E-value: 3.9e-168
                                 TIGR00133   3 eyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalnsk 71 
                                               ++e+viGlEvH q+  ++KlF+ + + +   +pN++v+ v+ ++PG lPv+N++ +++A+  +l l+++
  lcl|FitnessBrowser__azobra:AZOBR_RS20650  10 DWEIVIGLEVHAQVISNAKLFSGAATAFGA-EPNSQVSFVDAAFPGMLPVINERCIEQAVRTGLGLKAQ 77 
                                               5799************************99.************************************** PP

                                 TIGR00133  72 ivsevsvFdRKhYfYpDlPkgyqitqqdlPiaedGkleieleeke.keigierlhlEeDtgksqykesd 139
                                               i+   svFdRK+YfY DlP+gyqi+q+ +Pi+ +G++ ++l +++ +++g++rlhlE+D+gks + ++ 
  lcl|FitnessBrowser__azobra:AZOBR_RS20650  78 IN-LHSVFDRKNYFYADLPQGYQISQYLQPIVGKGEIVLDLPDGSsRTVGVTRLHLEQDAGKSLHDQH- 144
                                               77.59***********************************9887659******************966. PP

                                 TIGR00133 140 kdkqslvDfNRsgvPLlEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlkG 208
                                                +  +++D+NRsgv L+EiV++Pd+++a+ea a+++klr+ilryl+  dg++eeGsmR+DvNvs+r k 
  lcl|FitnessBrowser__azobra:AZOBR_RS20650 145 -PAKTYIDLNRSGVALMEIVSEPDMRTAEEAGAYVRKLRSILRYLGTCDGNMEEGSMRCDVNVSVR-KP 211
                                               .78***************************************************************.66 PP

                                 TIGR00133 209 qekegtrvEiKNlnslksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRYf 277
                                                  +gtr EiKN+ns++ + +aieyE  Rq++++++g ++ qetr +d++k +t s+R+Kee++DYRYf
  lcl|FitnessBrowser__azobra:AZOBR_RS20650 212 GAPFGTRCEIKNVNSIRFVMQAIEYEARRQIEIIEEGGKIDQETRLWDTTKFVTRSMRSKEEAHDYRYF 280
                                               6789***************************************************************** PP

                                 TIGR00133 278 pePdlppieideevvkekveeklpelPeakrirlkkeyglseedakvlvsdlelldafeevvklikepk 346
                                               p+Pdl p+e+d ++v++ ++++lpelP+ k++r+ +ey+ls +da+vlvs++  +d +e v+k   +pk
  lcl|FitnessBrowser__azobra:AZOBR_RS20650 281 PDPDLLPLELDADWVED-IKKTLPELPDDKKARFISEYKLSPYDANVLVSEKARADFYEAVAKGR-DPK 347
                                               ****************9.9*************************************999888765.56* PP

                                 TIGR00133 347 lavnWileellgeLnkkkislaeallkpeelaeliklikegkisqksakelleellenkkdpkkliekl 415
                                               la+nW++ el g+Lnk   +++e+ +++e+l  li+li +++is+++ake++e ++e++++p  ++ek+
  lcl|FitnessBrowser__azobra:AZOBR_RS20650 348 LAANWVTGELFGYLNKAGKEIEESPVSAENLGGLIDLIADNTISGRIAKEVFEAMFETGEKPADIVEKK 416
                                               ********************************************************************* PP

                                 TIGR00133 416 gliqisdekelvkiveevikenpkevekyksgkekalkflvGqvmkktkgradpkevekllkell 480
                                               gl q++d+ ++ + +++v++ n+++v +++sgk+k+++f+vGqvmk t+g+a+p+ v+++l  +l
  lcl|FitnessBrowser__azobra:AZOBR_RS20650 417 GLRQVTDTGAIESSIDAVLAANADKVAEFRSGKDKLFGFFVGQVMKATQGKANPALVNEILMAKL 481
                                               ************************************************************98877 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (481 nodes)
Target sequences:                          1  (483 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.03
# Mc/sec: 7.19
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis